##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632988.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	414476
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.596582190524902	33.0	33.0	33.0	27.0	33.0
2	31.146727434157828	33.0	33.0	33.0	27.0	33.0
3	31.583063916849227	33.0	33.0	33.0	27.0	33.0
4	31.72833891467781	33.0	33.0	33.0	27.0	33.0
5	31.838885725590867	33.0	33.0	33.0	33.0	33.0
6	34.70104662272363	37.0	37.0	37.0	27.0	37.0
7	34.86633242938071	37.0	37.0	37.0	27.0	37.0
8	35.02061398006157	37.0	37.0	37.0	33.0	37.0
9	35.110549223597985	37.0	37.0	37.0	33.0	37.0
10	35.141629430895875	37.0	37.0	37.0	33.0	37.0
11	35.17693907488009	37.0	37.0	37.0	33.0	37.0
12	35.11763286655922	37.0	37.0	37.0	33.0	37.0
13	35.15702477344888	37.0	37.0	37.0	33.0	37.0
14	35.129409181713775	37.0	37.0	37.0	33.0	37.0
15	35.15550478194154	37.0	37.0	37.0	33.0	37.0
16	35.149702274679356	37.0	37.0	37.0	33.0	37.0
17	35.09755450255262	37.0	37.0	37.0	33.0	37.0
18	35.1114612185024	37.0	37.0	37.0	33.0	37.0
19	35.12862505911078	37.0	37.0	37.0	33.0	37.0
20	35.114911357955584	37.0	37.0	37.0	33.0	37.0
21	35.113381715708506	37.0	37.0	37.0	33.0	37.0
22	35.117311979463224	37.0	37.0	37.0	33.0	37.0
23	35.119987647053144	37.0	37.0	37.0	33.0	37.0
24	35.12346191335566	37.0	37.0	37.0	33.0	37.0
25	35.1027369497872	37.0	37.0	37.0	33.0	37.0
26	35.05666190563507	37.0	37.0	37.0	33.0	37.0
27	34.9851282100773	37.0	37.0	37.0	27.0	37.0
28	34.97052905355196	37.0	37.0	37.0	27.0	37.0
29	35.009592835290825	37.0	37.0	37.0	27.0	37.0
30	34.963315125604375	37.0	37.0	37.0	27.0	37.0
31	34.97278491396366	37.0	37.0	37.0	27.0	37.0
32	34.99126125517521	37.0	37.0	37.0	27.0	37.0
33	34.98527297117324	37.0	37.0	37.0	27.0	37.0
34	34.92515851340005	37.0	37.0	37.0	27.0	37.0
35	34.917548905123574	37.0	37.0	37.0	27.0	37.0
36	34.903041430625656	37.0	37.0	37.0	27.0	37.0
37	34.91247502871095	37.0	37.0	37.0	27.0	37.0
38	34.78767166253294	37.0	37.0	37.0	27.0	37.0
39	34.864749708065126	37.0	37.0	37.0	27.0	37.0
40	34.89217952306044	37.0	37.0	37.0	27.0	37.0
41	34.89831015547342	37.0	37.0	37.0	27.0	37.0
42	34.82816857912159	37.0	37.0	37.0	27.0	37.0
43	34.6031181540065	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	7.0
16	20.0
17	16.0
18	15.0
19	22.0
20	28.0
21	96.0
22	305.0
23	692.0
24	1375.0
25	2437.0
26	3847.0
27	5823.0
28	7811.0
29	10641.0
30	13638.0
31	17146.0
32	21717.0
33	28153.0
34	39171.0
35	70023.0
36	191492.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.802845038072164	20.36716239299742	13.405842557832056	25.42415001109835
2	16.074513361449156	22.6312259334678	37.627751667165285	23.666509037917756
3	17.78824346886189	26.53302000598346	29.88423937694824	25.794497148206407
4	12.958530771383629	17.724066049662706	38.608990629131725	30.708412549821944
5	14.084048292301604	36.86148293266679	35.370202376012124	13.684266399019485
6	30.371119196286394	39.46042714174041	17.150570841254982	13.017882820718206
7	27.178654493866954	31.68217218849825	23.520300331020373	17.618872986614424
8	24.551481871085418	36.38087609415261	20.29719452996072	18.770447504801243
9	25.85819203041913	15.646744322952355	19.992954959997682	38.502108686630834
10	14.978189328212007	28.113087368146765	34.002692556384446	22.90603074725678
11	34.49777550449242	23.40497399125643	23.88244433935861	18.21480616489254
12	23.201102114477074	25.383616904235712	30.101863557841707	21.313417423445507
13	28.03539891332671	22.014302396278676	26.010914986633725	23.939383703760893
14	22.001032629151023	20.576342176627836	27.380596222700472	30.04202897152067
15	24.052297358592536	28.51745336280026	23.926113936633243	23.50413534197396
16	23.43054845153881	28.17316322296104	24.798782076646177	23.597506248853975
17	22.7011937965045	27.626448817301846	26.19500284696822	23.47735453922543
18	23.263590654223645	27.460938630946064	27.117372296586534	22.158098418243757
19	24.767417172526272	26.462569605960297	26.601781526554007	22.16823169495942
20	24.22914716413013	26.44109671006283	26.828815178683445	22.500940947123595
21	23.315945917254556	27.016522066416393	26.877310145822676	22.790221870506375
22	23.02956021579054	26.881170441714357	26.94414151844739	23.145127824047712
23	23.035350659628058	26.734961734816977	27.293498296644437	22.936189308910528
24	23.32318397205146	27.280469798010014	25.862052326310813	23.534293903627713
25	23.45443403236858	26.733272855364365	26.994084096546	22.818209015721056
26	23.348034626854147	26.905779828023817	26.888167228018027	22.858018317104005
27	23.159845202134743	27.06598210752854	26.354481320993255	23.41969136934346
28	23.00157307057586	26.645933660815103	27.188063965102927	23.164429303506115
29	24.1299858134126	26.62614964437024	26.291027707273763	22.952836834943398
30	23.580376185834645	26.54773738407049	26.87320858143777	22.998677848657103
31	23.499068703616132	26.56896901147473	26.69105086904911	23.240911415860026
32	22.671759040330443	26.55835319777261	27.061156737663943	23.708731024233007
33	22.68092724307318	26.707215858095523	27.479998841911236	23.131858056920063
34	23.66916299134329	26.20417104971096	27.03896003628678	23.087705922658973
35	23.251285961068916	27.112064389735473	26.787075729354655	22.849573919840957
36	24.077389281888458	26.608778312857677	26.402976288132486	22.910856117121376
37	23.247666933670466	26.388741447031915	26.701666682751235	23.661924936546384
38	23.42065644331638	26.680917592333454	26.60226406354047	23.296161900809697
39	23.04403632538434	26.64062575396404	26.91953213213793	23.39580578851369
40	22.99674770071126	26.943417712967697	27.238247811694766	22.821586774626276
41	23.150918267885235	26.18052673737442	27.056813904785805	23.611741089954545
42	22.822310580105963	26.820853318406858	26.95837635954796	23.39845974193922
43	23.49544967621768	25.92815989345583	27.09927715959428	23.477113270732204
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	11.5
2	17.0
3	53.5
4	90.0
5	90.0
6	125.5
7	161.0
8	137.0
9	113.0
10	161.5
11	210.0
12	210.0
13	346.0
14	482.0
15	843.0
16	1204.0
17	1506.0
18	1808.0
19	1808.0
20	1788.5
21	1769.0
22	2200.0
23	2631.0
24	3656.5
25	4682.0
26	4682.0
27	6180.5
28	7679.0
29	9554.5
30	11430.0
31	13935.5
32	16441.0
33	16441.0
34	19083.5
35	21726.0
36	23865.0
37	26004.0
38	28066.5
39	30129.0
40	30129.0
41	31671.5
42	33214.0
43	33826.5
44	34439.0
45	33751.5
46	33064.0
47	33064.0
48	31996.5
49	30929.0
50	30491.0
51	30053.0
52	28517.5
53	26982.0
54	26982.0
55	26109.5
56	25237.0
57	22045.5
58	18854.0
59	17817.0
60	16780.0
61	16780.0
62	14772.0
63	12764.0
64	10820.0
65	8876.0
66	7532.0
67	6188.0
68	6188.0
69	5126.0
70	4064.0
71	3364.5
72	2665.0
73	2162.0
74	1659.0
75	1659.0
76	1263.5
77	868.0
78	692.5
79	517.0
80	430.5
81	344.0
82	344.0
83	261.0
84	178.0
85	146.0
86	114.0
87	90.0
88	66.0
89	66.0
90	49.5
91	33.0
92	19.0
93	5.0
94	3.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	414476.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.099314823533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.74521546750903	61.32233582671677
2	6.461447491095758	8.929631888059065
3	1.8197153902960368	3.772232599298822
4	0.8072283097114172	2.231156924288705
5	0.4980714938812496	1.720819948016393
6	0.31222198948915303	1.2944595327924477
7	0.20734404956987765	1.0029132230610645
8	0.16622762044808753	0.9188971742167323
9	0.11522263675733256	0.7165624726883258
>10	0.7619578399203512	10.126296408569583
>50	0.06988064852099661	3.3534021184028506
>100	0.03476474472545961	4.232517592647888
>500	7.023180752618103E-4	0.3787742912413778
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	963	0.23234155898049585	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	598	0.14427855895154362	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	479	0.11556760825717292	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	427	0.10302164660921259	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.4126849323000607E-4	0.0	0.0	0.0	0.0
3	2.4126849323000607E-4	0.0	2.4126849323000607E-4	0.0	0.0
4	2.4126849323000607E-4	0.0	2.4126849323000607E-4	0.0	0.0
5	2.4126849323000607E-4	2.4126849323000607E-4	2.4126849323000607E-4	0.0	0.0
6	2.4126849323000607E-4	2.4126849323000607E-4	4.8253698646001213E-4	0.0	0.0
7	2.4126849323000607E-4	2.4126849323000607E-4	9.650739729200243E-4	0.0	0.0
8	2.4126849323000607E-4	2.4126849323000607E-4	0.002171416439070055	0.0	0.0
9	2.4126849323000607E-4	2.4126849323000607E-4	0.0031364904119900792	2.4126849323000607E-4	0.0
10	4.8253698646001213E-4	2.4126849323000607E-4	0.0031364904119900792	2.4126849323000607E-4	0.0
11	4.8253698646001213E-4	2.4126849323000607E-4	0.005307906851060134	4.8253698646001213E-4	0.0
12	4.8253698646001213E-4	2.4126849323000607E-4	0.006031712330750152	4.8253698646001213E-4	0.0
13	4.8253698646001213E-4	2.4126849323000607E-4	0.0062729808239801585	4.8253698646001213E-4	0.0
14	4.8253698646001213E-4	2.4126849323000607E-4	0.006996786303670176	4.8253698646001213E-4	0.0
15	4.8253698646001213E-4	2.4126849323000607E-4	0.0074793232901301885	4.8253698646001213E-4	0.0
16	4.8253698646001213E-4	2.4126849323000607E-4	0.008203128769820206	4.8253698646001213E-4	0.0
17	4.8253698646001213E-4	2.4126849323000607E-4	0.008203128769820206	4.8253698646001213E-4	0.0
18	4.8253698646001213E-4	2.4126849323000607E-4	0.00868566575628022	4.8253698646001213E-4	2.4126849323000607E-4
19	4.8253698646001213E-4	2.4126849323000607E-4	0.00868566575628022	4.8253698646001213E-4	2.4126849323000607E-4
20	4.8253698646001213E-4	2.4126849323000607E-4	0.00916820274274023	4.8253698646001213E-4	2.4126849323000607E-4
21	4.8253698646001213E-4	2.4126849323000607E-4	0.009409471235970237	4.8253698646001213E-4	2.4126849323000607E-4
22	4.8253698646001213E-4	2.4126849323000607E-4	0.009650739729200244	4.8253698646001213E-4	2.4126849323000607E-4
23	4.8253698646001213E-4	2.4126849323000607E-4	0.010133276715660255	9.650739729200243E-4	2.4126849323000607E-4
24	4.8253698646001213E-4	2.4126849323000607E-4	0.010133276715660255	0.0012063424661500305	2.4126849323000607E-4
25	4.8253698646001213E-4	2.4126849323000607E-4	0.010133276715660255	0.0014476109593800366	2.4126849323000607E-4
26	4.8253698646001213E-4	2.4126849323000607E-4	0.010133276715660255	0.0019301479458400485	2.4126849323000607E-4
27	4.8253698646001213E-4	2.4126849323000607E-4	0.010133276715660255	0.0031364904119900792	2.4126849323000607E-4
28	4.8253698646001213E-4	2.4126849323000607E-4	0.010133276715660255	0.007720591783360194	2.4126849323000607E-4
29	4.8253698646001213E-4	2.4126849323000607E-4	0.010133276715660255	0.017612600005790444	2.4126849323000607E-4
30	4.8253698646001213E-4	2.4126849323000607E-4	0.010133276715660255	0.04077437535587103	2.4126849323000607E-4
31	4.8253698646001213E-4	2.4126849323000607E-4	0.010133276715660255	0.09626612879877243	2.4126849323000607E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTCG	30	3.595542E-4	30.833334	21
GCCTAAG	25	0.005492338	29.6	1
GGTATCA	255	0.0	26.84314	1
CGCCGTC	35	8.859791E-4	26.42857	26
GTCCGGC	50	2.6980636E-4	22.2	15
GTATCAA	685	0.0	21.605839	1
GTCTAGA	60	3.7188893E-5	21.583334	1
TAACCGT	100	1.2838427E-8	20.35	7
ATAAGGC	65	6.888531E-5	19.923075	3
CTAACCG	105	2.2489985E-8	19.380953	6
TGGCACG	50	0.007027478	18.5	19
GCCTAAA	70	1.21681216E-4	18.5	11
CGCGTGT	50	0.007027478	18.5	14
CTGTGCG	50	0.007027478	18.5	9
TGCCGCG	50	0.007027478	18.5	11
TGTCCGG	50	0.007027478	18.5	14
GTACCCT	115	6.375558E-8	17.695652	1
GTATATA	95	3.5951398E-6	17.526316	1
TAGACAG	75	2.063251E-4	17.266666	5
ATAGCAG	75	2.063251E-4	17.266666	5
>>END_MODULE