FastQCFastQC Report
Fri 10 Feb 2017
ERR1632987.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632987.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339641
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12730.3748075173492011No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9620.2832402448467647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4930.14515326477074322No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCG250.005490958729.64
GGTATCA2050.027.9756091
GTATCAA6450.024.0930211
CCGCCGC551.8975463E-523.54545432
CGTTCTG400.001928402123.12510
TCGCCGT652.6736143E-622.7692325
CGCCGTC705.0844774E-621.14285726
GTCTTAT450.003820342720.5555551
CGAGTCG759.234285E-619.73333421
GGCGAGT759.234285E-619.73333419
GCCGTCC759.234285E-619.73333427
GCGAGTC851.2400287E-619.58823620
TCCGCCG701.2159906E-418.531
GAGTCGC801.611212E-518.522
CCGTCCG701.2159906E-418.528
GGCTTAG609.2185603E-418.51
AGCGCAA500.00702492818.510
GTATAGA609.2185603E-418.51
CGTCCGC701.2159906E-418.529
TAGACAG852.7139795E-517.4117645
Produced by FastQC (version 0.11.5)