FastQCFastQC Report
Fri 10 Feb 2017
ERR1632986.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632986.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247929
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5510.22224104481524953No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4610.1859403296911616No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC4230.17061336108321334No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC3950.15931980526683043No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT3630.14641288433382138No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG3480.1403627651464734No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA3470.13995942386731686No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA3340.13471598723828193No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC2890.11656562967623796No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT2840.1145489232804553No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA2760.11132219304720303No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT2630.10607875641816811No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT2580.10406205002238544No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC2540.10244868490575931No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2520.10164200234744625No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC2490.10043197850997665No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA2490.10043197850997665No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAC200.001839153237.01
TAGCACC303.5914104E-430.8333344
CATACTC250.00548813429.63
GGTATCA1350.026.0370371
GTATTAC450.003817475620.5555553
GTCAGGG450.003817475620.5555551
GTATCAA3300.019.0606061
TATAGGT500.007019707518.54
GGTACTG650.001575213217.0769235
GGACTTA1452.9413059E-916.58620623
GAACTTA803.3691985E-416.18756
AGATTGT700.002584810115.8571436
TAGATCT700.002584810115.85714329
ACTAGAT750.004092343614.827
CTACAGA750.004092343614.84
CTTTATT1051.6473801E-414.0952382
ACACCTC1854.825779E-914.05
CAACGCA5300.013.9622644
AACGCAG5300.013.9622645
CTGGTAC1203.2798816E-513.8750013
Produced by FastQC (version 0.11.5)