##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632986.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	247929
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.56180196749876	33.0	33.0	33.0	14.0	33.0
2	30.43626602777408	33.0	33.0	33.0	27.0	33.0
3	30.877037377636338	33.0	33.0	33.0	27.0	33.0
4	31.0699595448697	33.0	33.0	33.0	27.0	33.0
5	31.185593456191086	33.0	33.0	33.0	27.0	33.0
6	33.45162123027157	37.0	33.0	37.0	27.0	37.0
7	33.68728950626994	37.0	33.0	37.0	27.0	37.0
8	33.79676439625861	37.0	33.0	37.0	27.0	37.0
9	33.87273372618774	37.0	33.0	37.0	27.0	37.0
10	33.99155000020167	37.0	33.0	37.0	27.0	37.0
11	33.946581480988506	37.0	33.0	37.0	27.0	37.0
12	33.931952292793504	37.0	33.0	37.0	27.0	37.0
13	34.03082334055314	37.0	33.0	37.0	27.0	37.0
14	33.98127689782155	37.0	33.0	37.0	27.0	37.0
15	34.03317885362342	37.0	33.0	37.0	27.0	37.0
16	33.98860964227662	37.0	33.0	37.0	27.0	37.0
17	34.00768768478073	37.0	33.0	37.0	27.0	37.0
18	34.018267326533	37.0	33.0	37.0	27.0	37.0
19	34.017839784777095	37.0	33.0	37.0	27.0	37.0
20	34.0296496174308	37.0	33.0	37.0	27.0	37.0
21	33.991457231707464	37.0	33.0	37.0	27.0	37.0
22	34.00725207619924	37.0	33.0	37.0	27.0	37.0
23	33.97954253032118	37.0	33.0	37.0	27.0	37.0
24	33.99500260155125	37.0	33.0	37.0	27.0	37.0
25	33.9775742248789	37.0	33.0	37.0	27.0	37.0
26	33.876682437310684	37.0	33.0	37.0	27.0	37.0
27	33.81633451512328	37.0	33.0	37.0	27.0	37.0
28	33.78736654445426	37.0	33.0	37.0	27.0	37.0
29	33.84946900120599	37.0	33.0	37.0	27.0	37.0
30	33.79119425319345	37.0	33.0	37.0	27.0	37.0
31	33.779033513626885	37.0	33.0	37.0	27.0	37.0
32	33.82504265334027	37.0	33.0	37.0	27.0	37.0
33	33.786580028959904	37.0	33.0	37.0	27.0	37.0
34	33.689572417909964	37.0	33.0	37.0	27.0	37.0
35	33.71355105695582	37.0	33.0	37.0	27.0	37.0
36	33.65379201303599	37.0	33.0	37.0	27.0	37.0
37	33.67451568795905	37.0	33.0	37.0	27.0	37.0
38	33.55851070266084	37.0	33.0	37.0	27.0	37.0
39	33.64052208495174	37.0	33.0	37.0	27.0	37.0
40	33.66588015117231	37.0	33.0	37.0	27.0	37.0
41	33.69092764460793	37.0	33.0	37.0	27.0	37.0
42	33.583049986084724	37.0	33.0	37.0	27.0	37.0
43	33.253798466496455	37.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	11.0
16	23.0
17	20.0
18	17.0
19	36.0
20	113.0
21	267.0
22	650.0
23	1275.0
24	2220.0
25	3420.0
26	4956.0
27	6564.0
28	8252.0
29	10268.0
30	12510.0
31	15105.0
32	18383.0
33	22874.0
34	29892.0
35	46598.0
36	64474.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.078409544668034	21.89175126749997	13.216687035401264	24.813152152430735
2	15.781937570836813	25.126951667614517	36.61612800438835	22.474982757160316
3	18.393975694654518	27.916863295540256	30.901588761298598	22.78757224850663
4	14.014496085572887	19.71169165365891	37.39255996676468	28.881252294003524
5	14.57473712232131	37.91932367734311	34.99308269706247	12.512856503273115
6	29.19384178534984	39.98564105046203	17.724429171254673	13.09608799293346
7	25.278607988577374	33.21354097342385	24.075440952853437	17.432410085145346
8	23.50955313819682	37.159428707412204	21.33110688947239	17.999911264918588
9	25.371779824062536	16.092107014508187	20.255395697961916	38.28071746346736
10	14.147195366415385	29.43302316388966	34.51189655102872	21.90788491866623
11	32.731548144831784	24.20814023369594	24.886560265237225	18.17375135623505
12	21.59166535580751	26.28413779751461	30.983063699688216	21.141133146989663
13	27.311851376805457	22.477402804835258	27.423980252410974	22.786765565948315
14	21.371037676108887	21.787689217477585	27.9043597158864	28.936913390527124
15	23.4587321370231	29.474163974363627	24.63245525936861	22.434648629244663
16	22.485872971697543	29.156331046388278	25.218510137983053	23.139285843931127
17	22.051474414045956	28.497271396246504	26.681428957483792	22.76982523222374
18	21.796159384339873	28.327464717721607	28.0306055362624	21.845770361676127
19	23.980252410972497	27.23077977969499	28.09070338685672	20.69826442247579
20	23.889500623162277	26.56526666908671	28.20242892118308	21.34280378656793
21	22.32211641235999	28.126600760701653	26.93311391567747	22.618168911260884
22	22.105118804173777	27.808364491447147	27.277970709356307	22.80854599502277
23	21.896591362849847	27.984221289159393	27.537722493133117	22.581464854857643
24	23.130008994510526	27.978977852530363	27.108567372110564	21.78244578084855
25	23.164293003238832	27.22634302562427	27.513925357662878	22.09543861347402
26	22.954555538077432	27.34169863146304	27.076703411057196	22.62704241940233
27	22.593565093232336	27.155758301771876	27.19367238201259	23.057004222983192
28	21.759455327936628	27.869269024599785	27.92210673216929	22.449168915294297
29	22.74320470779941	27.899519620536523	26.979498162780473	22.377777508883593
30	22.14303288441449	28.331901471792325	27.215856152366204	22.30920949142698
31	23.15178942358498	27.379209370424597	27.511101968708786	21.95789923728164
32	22.004283484384644	27.64944802745947	27.61153394721876	22.734734540937122
33	22.384230969350096	27.176732048288017	27.7652069745774	22.67383000778449
34	22.069221430328845	27.563536334999135	27.4227702285735	22.944472006098522
35	22.67100661883039	27.755123442598485	27.305397916338954	22.26847202223217
36	22.801285851997953	27.76641699841487	27.0460494738413	22.386247675745878
37	23.271178442215312	26.912946851719642	27.160598397121756	22.655276308943286
38	23.165099685797145	27.62565089198924	27.086383601756953	22.122865820456663
39	22.68149349208846	27.30418789250148	27.526428937316734	22.487889678093325
40	22.533063901358858	27.13801128548899	27.682925353629468	22.645999459522688
41	22.28984911002747	27.561116287324193	27.769240387368967	22.379794215279375
42	22.725457691516525	27.13801128548899	27.392116291357603	22.74441473163688
43	22.335830015851315	27.067426561636598	27.718822727474397	22.877920695037695
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	5.0
2	8.0
3	31.0
4	54.0
5	54.0
6	85.5
7	117.0
8	119.0
9	121.0
10	170.0
11	219.0
12	219.0
13	314.5
14	410.0
15	768.0
16	1126.0
17	1235.5
18	1345.0
19	1345.0
20	1472.0
21	1599.0
22	2017.0
23	2435.0
24	3160.5
25	3886.0
26	3886.0
27	4863.5
28	5841.0
29	7781.5
30	9722.0
31	10491.0
32	11260.0
33	11260.0
34	12421.0
35	13582.0
36	14652.5
37	15723.0
38	17186.0
39	18649.0
40	18649.0
41	19849.0
42	21049.0
43	19649.0
44	18249.0
45	18594.5
46	18940.0
47	18940.0
48	18615.0
49	18290.0
50	18652.0
51	19014.0
52	19042.5
53	19071.0
54	19071.0
55	16125.0
56	13179.0
57	11729.0
58	10279.0
59	8854.5
60	7430.0
61	7430.0
62	6590.0
63	5750.0
64	4698.0
65	3646.0
66	3103.0
67	2560.0
68	2560.0
69	2095.0
70	1630.0
71	1341.5
72	1053.0
73	918.0
74	783.0
75	783.0
76	579.5
77	376.0
78	314.5
79	253.0
80	188.0
81	123.0
82	123.0
83	95.5
84	68.0
85	62.5
86	57.0
87	38.0
88	19.0
89	19.0
90	15.0
91	11.0
92	5.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	247929.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.66597486226202
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.77033282622729	63.01499366947141
2	4.507956812857775	6.190864983837095
3	1.1638701079325358	2.39754826722701
4	0.5752525363633991	1.5800110480552652
5	0.3445436215853804	1.182921182936722
6	0.25736457054792183	1.0603313479008276
7	0.17324458729484135	0.8327205933347372
8	0.1294108630510705	0.710889845533475
9	0.10403666906095327	0.64294013722436
>10	0.8206697685833927	11.563410818927114
>50	0.10808599660980427	5.194474507632322
>100	0.04464421599100611	5.406642048478308
>500	5.874238946185015E-4	0.22225154944132702
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	551	0.22224104481524953	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	461	0.1859403296911616	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	423	0.17061336108321334	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	395	0.15931980526683043	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	363	0.14641288433382138	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	348	0.1403627651464734	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	347	0.13995942386731686	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	334	0.13471598723828193	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	289	0.11656562967623796	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	284	0.1145489232804553	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	276	0.11132219304720303	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	263	0.10607875641816811	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	258	0.10406205002238544	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	254	0.10244868490575931	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	252	0.10164200234744625	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	249	0.10043197850997665	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	249	0.10043197850997665	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0012100238374695982	0.0	0.0
2	0.0	0.0	0.0012100238374695982	0.0	0.0
3	0.0	0.0	0.0012100238374695982	0.0	0.0
4	0.0	0.0	0.0012100238374695982	0.0	0.0
5	0.0	0.0	0.0012100238374695982	0.0	0.0
6	0.0	0.0	0.002823388954095729	0.0	0.0
7	0.0	0.0	0.005646777908191458	0.0	0.0
8	0.0	0.0	0.006453460466504524	0.0	0.0
9	0.0	0.0	0.008066825583130655	0.0	0.0
10	0.0	0.0	0.010890214537226384	0.0	0.0
11	0.0	0.0	0.015730309887104776	0.0	0.0
12	0.0	0.0	0.01774701628288744	0.0	0.0
13	0.0	0.0	0.018957040120357038	0.0	0.0
14	0.0	0.0	0.02057040523698317	0.0	0.0
15	0.0	0.0	0.021377087795296235	4.0334127915653273E-4	0.0
16	0.0	0.0	0.021377087795296235	4.0334127915653273E-4	0.0
17	0.0	0.0	0.021780429074452767	4.0334127915653273E-4	0.0
18	0.0	0.0	0.022990452911922366	4.0334127915653273E-4	0.0
19	0.0	0.0	0.022990452911922366	4.0334127915653273E-4	0.0
20	0.0	0.0	0.024603818028548496	4.0334127915653273E-4	0.0
21	0.0	0.0	0.025410500586861562	4.0334127915653273E-4	0.0
22	0.0	0.0	0.025410500586861562	8.066825583130655E-4	0.0
23	0.0	0.0	0.025410500586861562	8.066825583130655E-4	0.0
24	0.0	0.0	0.025813841866018095	8.066825583130655E-4	0.0
25	0.0	0.0	0.025813841866018095	8.066825583130655E-4	0.0
26	0.0	0.0	0.026217183145174627	0.0012100238374695982	0.0
27	0.0	0.0	0.026217183145174627	0.0020167063957826636	0.0
28	0.0	0.0	0.026217183145174627	0.006856801745661056	0.0
29	0.0	0.0	0.026217183145174627	0.015730309887104776	0.0
30	0.0	0.0	0.026217183145174627	0.03549403256577488	0.0
31	0.0	0.0	0.026217183145174627	0.08833174013528067	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACATAC	20	0.0018391532	37.0	1
TAGCACC	30	3.5914104E-4	30.833334	4
CATACTC	25	0.005488134	29.6	3
GGTATCA	135	0.0	26.037037	1
GTATTAC	45	0.0038174756	20.555555	3
GTCAGGG	45	0.0038174756	20.555555	1
GTATCAA	330	0.0	19.060606	1
TATAGGT	50	0.0070197075	18.5	4
GGTACTG	65	0.0015752132	17.076923	5
GGACTTA	145	2.9413059E-9	16.586206	23
GAACTTA	80	3.3691985E-4	16.1875	6
AGATTGT	70	0.0025848101	15.857143	6
TAGATCT	70	0.0025848101	15.857143	29
ACTAGAT	75	0.0040923436	14.8	27
CTACAGA	75	0.0040923436	14.8	4
CTTTATT	105	1.6473801E-4	14.095238	2
ACACCTC	185	4.825779E-9	14.0	5
CAACGCA	530	0.0	13.962264	4
AACGCAG	530	0.0	13.962264	5
CTGGTAC	120	3.2798816E-5	13.875001	3
>>END_MODULE