FastQCFastQC Report
Fri 10 Feb 2017
ERR1632985.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632985.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242779
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5660.23313383777015312No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4370.1799990938260723No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA3860.1589923345923659No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC3850.1585804373524893No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG3520.14498782843656166No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA3500.14416403395680844No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC3430.14128075327767228No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT3400.1400450615580425No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC2890.11903830232433611No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA2610.10750517960779146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2520.10379810444890208No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT2440.10050292652988932No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA2430.10009102929001273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAT250.005487912329.61
TCCGTGC250.005487912329.68
GGTATCA851.8189894E-1228.2941191
GTATCAA2950.026.96611
AACCGAC400.001926825523.1250027
CCCACCG400.001926825523.12500219
TATTCCG400.001926825523.1250025
ATTATTG400.001926825523.1250023
ATTATTC656.873509E-519.9230753
TCGCCTT609.208232E-418.514
ATTTAAT801.6081269E-518.51
GGTGTAT500.00701929718.51
ATTCCGA609.208232E-418.56
TGGATAG500.00701929718.55
GTGACAC500.00701929718.524
GTATTAG1054.75884E-717.6190471
AATTGTA752.0588154E-417.2666665
CGTGCCC650.001575085917.07692116
AAACCGA650.001575085917.0769216
GGATTAC650.001575085917.0769211
Produced by FastQC (version 0.11.5)