##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632985.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	242779
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.63481602609781	33.0	33.0	33.0	27.0	33.0
2	30.48771928379308	33.0	33.0	33.0	27.0	33.0
3	30.937037387912465	33.0	33.0	33.0	27.0	33.0
4	31.118877662400784	33.0	33.0	33.0	27.0	33.0
5	31.235077992742372	33.0	33.0	33.0	27.0	33.0
6	33.627158856408506	37.0	33.0	37.0	27.0	37.0
7	33.79523352514015	37.0	33.0	37.0	27.0	37.0
8	33.973276107076806	37.0	33.0	37.0	27.0	37.0
9	34.00409425856437	37.0	33.0	37.0	27.0	37.0
10	34.140403412156736	37.0	33.0	37.0	27.0	37.0
11	34.15080381746362	37.0	33.0	37.0	27.0	37.0
12	34.05883128277157	37.0	33.0	37.0	27.0	37.0
13	34.1782238167222	37.0	33.0	37.0	27.0	37.0
14	34.096272741876355	37.0	33.0	37.0	27.0	37.0
15	34.180987647201775	37.0	33.0	37.0	27.0	37.0
16	34.14192331297188	37.0	33.0	37.0	27.0	37.0
17	34.17084261818361	37.0	33.0	37.0	27.0	37.0
18	34.10656193492847	37.0	33.0	37.0	27.0	37.0
19	34.137730199069935	37.0	33.0	37.0	27.0	37.0
20	34.0537567087763	37.0	33.0	37.0	27.0	37.0
21	34.137569559146385	37.0	33.0	37.0	27.0	37.0
22	34.16602753944946	37.0	37.0	37.0	27.0	37.0
23	34.1164845394371	37.0	33.0	37.0	27.0	37.0
24	34.11122873065628	37.0	33.0	37.0	27.0	37.0
25	34.10823423772237	37.0	33.0	37.0	27.0	37.0
26	34.0570642436125	37.0	33.0	37.0	27.0	37.0
27	33.9626162065088	37.0	33.0	37.0	27.0	37.0
28	33.93723097961521	37.0	33.0	37.0	27.0	37.0
29	33.99809703475177	37.0	33.0	37.0	27.0	37.0
30	33.95690731076411	37.0	33.0	37.0	27.0	37.0
31	33.96231140255129	37.0	33.0	37.0	27.0	37.0
32	33.9669576034171	37.0	33.0	37.0	27.0	37.0
33	33.92541364780315	37.0	33.0	37.0	27.0	37.0
34	33.853475794858696	37.0	33.0	37.0	27.0	37.0
35	33.87168165286124	37.0	33.0	37.0	27.0	37.0
36	33.84754035563208	37.0	33.0	37.0	27.0	37.0
37	33.89265134134336	37.0	33.0	37.0	27.0	37.0
38	33.72757116554562	37.0	33.0	37.0	27.0	37.0
39	33.78948343967147	37.0	33.0	37.0	27.0	37.0
40	33.83618846770108	37.0	33.0	37.0	27.0	37.0
41	33.856626808743755	37.0	33.0	37.0	27.0	37.0
42	33.69137363610526	37.0	33.0	37.0	27.0	37.0
43	33.44032638737288	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	12.0
16	22.0
17	13.0
18	25.0
19	21.0
20	73.0
21	206.0
22	582.0
23	1099.0
24	1946.0
25	3113.0
26	4561.0
27	5864.0
28	7868.0
29	9715.0
30	11957.0
31	14191.0
32	17284.0
33	21643.0
34	28709.0
35	44885.0
36	68987.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.053299502840034	21.874214820886483	13.411374130381953	24.661111545891533
2	15.746831480482248	24.589029528913127	37.30759250182265	22.356546488781976
3	18.086407802981313	27.786587802075136	31.23252011088274	22.894484284060812
4	13.74336330572249	19.4963320550789	38.12932749537645	28.63097714382216
5	13.93819070018412	38.2500133866603	35.35272820136832	12.459067711787263
6	28.77019841090045	40.56158069684775	17.690986452699782	12.97723443955202
7	25.109255742877266	33.441936905580796	24.236033594338885	17.212773757203053
8	23.476083186766566	37.40150507251451	21.1805798689343	17.94183187178463
9	25.186692423974065	16.45241145239086	20.47994266390421	37.88095345973087
10	13.995444416526965	29.509965853718818	34.80490487233245	21.68968485742177
11	32.66592250565329	24.507061978177685	24.87776949406662	17.949246022102404
12	21.639433394156825	26.64563244761697	30.794261447654037	20.920672710572166
13	26.793915453972545	22.852058868353524	27.61812183096561	22.73590384670832
14	21.483324340243595	21.86391738988957	27.90150713200071	28.751251137866124
15	23.134620374908867	29.587402534815617	24.990629337792807	22.28734775248271
16	22.38579119281322	29.775639573439218	25.330856457930878	22.507712775816692
17	21.72716750625054	28.80768105972922	26.751490038265253	22.713661395754986
18	21.813665926624626	28.792440861853784	27.881736064486635	21.51215714703496
19	23.87603540668674	27.517207007195843	28.33894200074965	20.267815585367764
20	23.51892049971373	27.074005577088627	28.28251207888656	21.12456184431108
21	21.704101260817453	28.712944694557603	27.350388625045824	22.232565419579124
22	21.676092248505842	28.186128124755438	27.411761313787437	22.726018312951286
23	21.666206714748803	28.446035283117567	27.826541834343168	22.06121616779046
24	22.826109342241296	28.436561646600406	26.856111937193912	21.881217073964386
25	22.96986147895823	27.541920841588443	27.75157653668563	21.736641142767702
26	22.391969651411365	28.091391759583818	27.351624316765456	22.16501427223936
27	22.22679885822085	27.84837238805663	27.23093842548161	22.693890328240908
28	21.588770033652004	28.35377030138521	28.011071797807887	22.046387867154902
29	22.711190012315726	28.2940452016031	27.299725264541003	21.695039521540167
30	21.894809682880314	28.61903212386574	27.552630169825232	21.933528023428714
31	22.68318100000412	27.81459681438675	27.693499025863026	21.80872315974611
32	21.72716750625054	27.85043187425601	27.957113259384048	22.4652873601094
33	21.953299090942792	27.578579695937457	28.339765795229404	22.128355417890344
34	21.95947754954094	27.647778432236723	27.86278879145231	22.529955226770028
35	22.198789845909243	28.263564805852237	27.64736653499685	21.89027881324167
36	22.622220208502384	28.08315381478629	27.130847396191598	22.163778580519732
37	22.740434716346964	27.1448519023474	27.55427775878474	22.560435622520895
38	22.859884915911177	28.004893339209737	27.276659019107914	21.858562725771176
39	22.076868262905773	27.771347604199704	28.078622945147647	22.07316118774688
40	22.265105301529374	27.573636929058935	28.017250256406033	22.144007513005658
41	21.851972369933147	27.786587802075136	28.17088792688	22.19055190111171
42	22.39114585693161	27.449243962616205	27.70379645685994	22.45581372359224
43	22.053390120232805	27.268009177070507	27.93404701395096	22.744553688745732
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	5.0
2	7.0
3	31.5
4	56.0
5	56.0
6	73.5
7	91.0
8	99.5
9	108.0
10	144.5
11	181.0
12	181.0
13	274.5
14	368.0
15	704.5
16	1041.0
17	1223.0
18	1405.0
19	1405.0
20	1525.5
21	1646.0
22	2090.5
23	2535.0
24	3255.0
25	3975.0
26	3975.0
27	5066.5
28	6158.0
29	8015.0
30	9872.0
31	10827.5
32	11783.0
33	11783.0
34	12816.5
35	13850.0
36	15124.5
37	16399.0
38	17726.0
39	19053.0
40	19053.0
41	20205.0
42	21357.0
43	19964.5
44	18572.0
45	18644.5
46	18717.0
47	18717.0
48	18177.0
49	17637.0
50	17867.0
51	18097.0
52	17867.5
53	17638.0
54	17638.0
55	14989.0
56	12340.0
57	10674.5
58	9009.0
59	7829.0
60	6649.0
61	6649.0
62	5816.0
63	4983.0
64	4116.0
65	3249.0
66	2746.0
67	2243.0
68	2243.0
69	1840.5
70	1438.0
71	1227.5
72	1017.0
73	802.5
74	588.0
75	588.0
76	457.5
77	327.0
78	252.5
79	178.0
80	130.5
81	83.0
82	83.0
83	65.5
84	48.0
85	44.0
86	40.0
87	31.5
88	23.0
89	23.0
90	17.5
91	12.0
92	7.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	242779.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.64405051532852
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.0438307433828	58.854419936842575
2	4.351529661218613	5.626010064775328
3	1.2831126599063547	2.4883679881153182
4	0.6892874580425951	1.7823333302915163
5	0.4687555087503439	1.5151127393497876
6	0.28606549726269925	1.1095459473445504
7	0.2344694110103817	1.060993671475811
8	0.17570204259854588	0.9086473373909443
9	0.1450967535001172	0.8441677676584571
>10	1.1465444491733	14.787981075326293
>50	0.13170436136573468	5.8959809219519865
>100	0.043265200835120574	4.893643600236118
>500	6.362529534576555E-4	0.23279561924135508
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	566	0.23313383777015312	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	437	0.1799990938260723	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	386	0.1589923345923659	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	385	0.1585804373524893	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	352	0.14498782843656166	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	350	0.14416403395680844	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	343	0.14128075327767228	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	340	0.1400450615580425	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	289	0.11903830232433611	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	261	0.10750517960779146	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	252	0.10379810444890208	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	244	0.10050292652988932	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	243	0.10009102929001273	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.118972398765956E-4	0.0	0.0
2	0.0	0.0	4.118972398765956E-4	0.0	0.0
3	0.0	0.0	4.118972398765956E-4	0.0	0.0
4	0.0	0.0	4.118972398765956E-4	0.0	0.0
5	0.0	0.0	4.118972398765956E-4	0.0	0.0
6	0.0	0.0	8.237944797531911E-4	0.0	0.0
7	0.0	0.0	0.0012356917196297867	0.0	0.0
8	0.0	0.0	0.0012356917196297867	0.0	0.0
9	0.0	0.0	0.0012356917196297867	4.118972398765956E-4	0.0
10	0.0	0.0	0.0016475889595063823	4.118972398765956E-4	0.0
11	0.0	0.0	0.002059486199382978	4.118972398765956E-4	0.0
12	0.0	0.0	0.002059486199382978	4.118972398765956E-4	0.0
13	0.0	0.0	0.002883280679136169	4.118972398765956E-4	0.0
14	0.0	0.0	0.0032951779190127646	4.118972398765956E-4	0.0
15	0.0	0.0	0.004118972398765956	4.118972398765956E-4	0.0
16	0.0	0.0	0.005766561358272338	4.118972398765956E-4	0.0
17	0.0	0.0	0.005766561358272338	4.118972398765956E-4	0.0
18	0.0	0.0	0.0061784585981489335	4.118972398765956E-4	0.0
19	0.0	0.0	0.0061784585981489335	4.118972398765956E-4	0.0
20	0.0	0.0	0.0061784585981489335	4.118972398765956E-4	0.0
21	0.0	0.0	0.006590355838025529	4.118972398765956E-4	0.0
22	0.0	0.0	0.006590355838025529	8.237944797531911E-4	0.0
23	0.0	0.0	0.006590355838025529	8.237944797531911E-4	0.0
24	0.0	0.0	0.007002253077902125	8.237944797531911E-4	0.0
25	0.0	0.0	0.007002253077902125	8.237944797531911E-4	0.0
26	0.0	0.0	0.007002253077902125	0.0012356917196297867	0.0
27	0.0	0.0	0.007002253077902125	0.002059486199382978	0.0
28	0.0	0.0	0.007002253077902125	0.009885533757038293	0.0
29	0.0	0.0	0.007002253077902125	0.02224245095333616	0.0
30	0.0	0.0	0.007002253077902125	0.03995403226802977	0.0
31	0.0	0.0	0.007002253077902125	0.10709328236791485	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACTAT	25	0.0054879123	29.6	1
TCCGTGC	25	0.0054879123	29.6	8
GGTATCA	85	1.8189894E-12	28.294119	1
GTATCAA	295	0.0	26.9661	1
AACCGAC	40	0.0019268255	23.125002	7
CCCACCG	40	0.0019268255	23.125002	19
TATTCCG	40	0.0019268255	23.125002	5
ATTATTG	40	0.0019268255	23.125002	3
ATTATTC	65	6.873509E-5	19.923075	3
TCGCCTT	60	9.208232E-4	18.5	14
ATTTAAT	80	1.6081269E-5	18.5	1
GGTGTAT	50	0.007019297	18.5	1
ATTCCGA	60	9.208232E-4	18.5	6
TGGATAG	50	0.007019297	18.5	5
GTGACAC	50	0.007019297	18.5	24
GTATTAG	105	4.75884E-7	17.619047	1
AATTGTA	75	2.0588154E-4	17.266666	5
CGTGCCC	65	0.0015750859	17.076921	16
AAACCGA	65	0.0015750859	17.076921	6
GGATTAC	65	0.0015750859	17.076921	1
>>END_MODULE