FastQCFastQC Report
Fri 10 Feb 2017
ERR1632984.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632984.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224350
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10140.4519723646088701No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7930.35346556719411637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4550.20280811232449297No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTG200.001838780737.07
CGGGATC352.379811E-531.71428717
GCCTAAA250.00548703629.611
TCCCGAA250.00548703629.622
CGCACTT250.00548703629.619
GGACCGT250.00548703629.66
CAATCGC250.00548703629.615
AATCGCA250.00548703629.616
GGTATCA2100.028.1904751
CCCCTAT601.3294066E-624.6666661
GTATCAA5700.024.0175441
ATTACCG400.001926372523.1255
TAATAGT603.7091075E-521.5833324
TTAGCCG450.003816360420.55555729
ACCGACC450.003816360420.5555578
ACTGTTC450.003816360420.55555727
TATAATA450.003816360420.5555572
GGCCTAA450.003816360420.55555710
GTCCTAG555.125381E-420.1818181
CCTACCG555.125381E-420.18181824
Produced by FastQC (version 0.11.5)