FastQCFastQC Report
Fri 10 Feb 2017
ERR1632983.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632983.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341971
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8870.2593787192481234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6570.19212155416687382No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC3480.10176301499249937No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGC358.856604E-426.4285723
TCGTGTA358.856604E-426.42857213
GAGTTAG451.3205582E-424.6666661
GGTATCA2000.023.1250021
TTAACGG753.72489E-722.235
GTATCAA4800.021.9687481
CACATGT705.0846447E-621.14285928
GTTAGCC450.003820396320.5555553
CGTGTAG450.003820396320.55555514
TAAGGCT450.003820396320.5555554
TAACGGC851.2400797E-619.58823636
GGCCGTT500.00702502618.514
CTGACGC500.00702502618.537
AACGGCC1103.8280632E-818.537
ATTCAAC500.00702502618.51
GTCGTGT500.00702502618.512
TATTAGA1002.86027E-718.52
AGTTAGC500.00702502618.52
GCCGTTA500.00702502618.515
ATACAAT701.2160214E-418.56
Produced by FastQC (version 0.11.5)