FastQCFastQC Report
Fri 10 Feb 2017
ERR1632982.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632982.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365872
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7900.21592250841824465No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG7610.20799623912187867No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG6800.18585734901823586No Hit
CTTTAAAGCCTTAGGCCGTATGACAAAATGAAGAGACTGAAAT6700.18312415270914417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6410.17519788341277825No Hit
GTGTGGGGTCATGGAGCCCAATCCAGACGTATGAGTAGCTGTT6360.17383128525823238No Hit
GATTATAACTGTAATGTGAGGTTACCCTATGTCTGCAAGTTCA3760.10276818122184808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGGC200.001840296637.07
ACGGGTA250.005491506329.616
GGTATCA1850.026.01
GTATCAA5500.025.2272741
AGCCTTA1400.021.1428597
CCATCGG450.003820920.55555319
TACAACC450.003820920.5555534
AAGCCTT1600.019.656256
CAACGCA7700.019.220785
AAAGCCT1650.019.0606065
AACGCAG7800.018.974366
GACGTAT2000.018.526
GCACCGC701.2163165E-418.510
ATATAAG609.2204165E-418.51
TCGTCTT500.007025940318.514
TTTATAC701.2163165E-418.53
CGTAAAC500.007025940318.53
CCTCTAT609.2204165E-418.51
ACGCAGA8050.018.3850947
TCAACGC8050.018.3850944
Produced by FastQC (version 0.11.5)