##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632982.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	365872
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.587877727729918	33.0	33.0	33.0	27.0	33.0
2	31.097952289325228	33.0	33.0	33.0	27.0	33.0
3	31.54628121310185	33.0	33.0	33.0	27.0	33.0
4	31.70082979839944	33.0	33.0	33.0	27.0	33.0
5	31.80758844623256	33.0	33.0	33.0	33.0	33.0
6	34.68269777408493	37.0	37.0	37.0	27.0	37.0
7	34.8508604101981	37.0	37.0	37.0	27.0	37.0
8	35.0189984475445	37.0	37.0	37.0	33.0	37.0
9	35.11774336379936	37.0	37.0	37.0	33.0	37.0
10	35.157915883150395	37.0	37.0	37.0	33.0	37.0
11	35.18495266103993	37.0	37.0	37.0	33.0	37.0
12	35.135479074649055	37.0	37.0	37.0	33.0	37.0
13	35.17348690252329	37.0	37.0	37.0	33.0	37.0
14	35.141904819171735	37.0	37.0	37.0	33.0	37.0
15	35.15918681943412	37.0	37.0	37.0	33.0	37.0
16	35.16416943630559	37.0	37.0	37.0	33.0	37.0
17	35.10361000568505	37.0	37.0	37.0	33.0	37.0
18	35.1216026369878	37.0	37.0	37.0	33.0	37.0
19	35.13054018891853	37.0	37.0	37.0	33.0	37.0
20	35.13649035728342	37.0	37.0	37.0	33.0	37.0
21	35.12845202693838	37.0	37.0	37.0	33.0	37.0
22	35.14027856736782	37.0	37.0	37.0	33.0	37.0
23	35.118158809638345	37.0	37.0	37.0	33.0	37.0
24	35.1244287619714	37.0	37.0	37.0	33.0	37.0
25	35.11901156688678	37.0	37.0	37.0	33.0	37.0
26	35.07519296365942	37.0	37.0	37.0	33.0	37.0
27	35.01973914374426	37.0	37.0	37.0	27.0	37.0
28	34.987011851139194	37.0	37.0	37.0	27.0	37.0
29	35.02678259063279	37.0	37.0	37.0	27.0	37.0
30	34.99808402938733	37.0	37.0	37.0	27.0	37.0
31	34.97200113700966	37.0	37.0	37.0	27.0	37.0
32	35.00844011020248	37.0	37.0	37.0	27.0	37.0
33	34.98621922420956	37.0	37.0	37.0	27.0	37.0
34	34.91648445357939	37.0	37.0	37.0	27.0	37.0
35	34.91695183014825	37.0	37.0	37.0	27.0	37.0
36	34.905808588796084	37.0	37.0	37.0	27.0	37.0
37	34.93485973236542	37.0	37.0	37.0	27.0	37.0
38	34.7837604408099	37.0	37.0	37.0	27.0	37.0
39	34.85190449118817	37.0	37.0	37.0	27.0	37.0
40	34.88784602265273	37.0	37.0	37.0	27.0	37.0
41	34.91290123321817	37.0	37.0	37.0	27.0	37.0
42	34.82465725718284	37.0	37.0	37.0	27.0	37.0
43	34.63040626229938	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	7.0
16	12.0
17	20.0
18	8.0
19	19.0
20	33.0
21	100.0
22	247.0
23	606.0
24	1200.0
25	2141.0
26	3416.0
27	5023.0
28	6991.0
29	9533.0
30	12163.0
31	15002.0
32	18862.0
33	24778.0
34	34330.0
35	61645.0
36	169733.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.583941925044826	19.898215769449425	12.969016486640136	25.548825818865613
2	16.27044430839201	22.727347268990247	36.82872698648708	24.17348143613067
3	18.75273319630909	24.936863165259982	29.860989635719598	26.44941400271133
4	13.271581318056588	17.092589758166792	38.07205798749289	31.563770936283724
5	14.566843048935146	36.47450474482879	34.93434862465561	14.024303581580444
6	31.48040844885643	38.675001093278524	16.402457690121135	13.442132767743912
7	27.88898849870993	31.314503432894565	22.437901779857437	18.358606288538066
8	25.71473083482748	35.289937464468444	20.17782175186951	18.817509948834566
9	25.949512397778456	15.023013512922553	19.791621113394847	39.23585297590414
10	14.58214894826606	28.05407355577907	33.9293523418026	23.434425154152272
11	34.98491275637382	23.028272182621244	23.68341933791053	18.303395723094415
12	23.66674684042507	24.827535312896313	30.124743079546946	21.380974767131676
13	28.652096908208335	21.83741855074999	25.888015480823896	23.62246906021778
14	22.917304412472124	19.855304587396684	26.46362662351861	30.763764376612585
15	23.89332881444877	28.90765076310841	23.81707263742511	23.38194778501771
16	24.0849258757161	27.610202475182575	24.202999956268858	24.101871692832468
17	22.69946866663751	27.043337560676957	25.91890059911663	24.338293173568896
18	24.234431713823415	26.588260375213192	26.744325884462327	22.432982026501072
19	24.94014300083089	26.01866226439848	26.223105348318537	22.818089386452094
20	23.977237941137886	26.97063453885512	26.55436874098045	22.497758779026544
21	23.454104167577732	26.570767918835003	27.24586740718065	22.72926050640661
22	23.17832465999038	26.220098832378536	27.226735033017015	23.374841474614072
23	23.436885030830453	26.30865439279311	26.78915030393143	23.46531027244501
24	23.18761752744129	26.790790221716886	25.717737350767482	24.30385490007434
25	23.944986224690602	27.229468229326102	26.108857742598506	22.71668780338479
26	24.139316482267024	26.210532645296713	26.536329645340444	23.113821227095816
27	23.648161105523243	26.48740543140771	26.319860497660386	23.544572965408666
28	23.29393886386496	26.027135172956662	27.008899287182402	23.670026675995974
29	23.781541085406918	26.036701360038485	26.273942799667644	23.907814754886957
30	23.667020160055976	26.458980189793152	26.681735688984126	23.19226396116675
31	23.598416932697774	26.387370446494952	26.92116368566056	23.09304893514672
32	23.40217343770499	26.481665719158613	26.762638299733236	23.35352254340316
33	23.604703284208686	26.264103292954914	26.944942493549657	23.186250929286743
34	23.938699873179694	25.92464031136572	26.512277517820436	23.624382297634146
35	23.45547076573228	26.874699348406	26.622698648707743	23.047131237153977
36	23.999650150872437	25.938306292911186	26.935922945729658	23.126120610486726
37	24.099685135785194	26.04380767044212	26.34801241964403	23.508494774128657
38	24.09503870205974	26.207799448987622	26.652763808107753	23.044398040844886
39	23.8047732540342	26.059386889403946	26.441214413784053	23.694625442777802
40	23.19554379673766	27.005072812349674	26.868686316526	22.93069707438667
41	23.990083963790614	25.63601478112564	26.745692482616874	23.628208772466873
42	23.380854506494074	26.46362662351861	26.43547470153496	23.720044168452358
43	23.976417982245156	25.5007215638256	26.935922945729658	23.58693750819959
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	8.5
2	10.0
3	52.0
4	94.0
5	94.0
6	125.0
7	156.0
8	129.0
9	102.0
10	158.5
11	215.0
12	215.0
13	338.0
14	461.0
15	824.0
16	1187.0
17	1368.5
18	1550.0
19	1550.0
20	1627.0
21	1704.0
22	1957.5
23	2211.0
24	2998.5
25	3786.0
26	3786.0
27	4957.0
28	6128.0
29	7423.0
30	8718.0
31	10383.0
32	12048.0
33	12048.0
34	14305.5
35	16563.0
36	19320.5
37	22078.0
38	23012.0
39	23946.0
40	23946.0
41	25210.5
42	26475.0
43	28111.0
44	29747.0
45	29712.5
46	29678.0
47	29678.0
48	28826.5
49	27975.0
50	28444.5
51	28914.0
52	27974.5
53	27035.0
54	27035.0
55	25409.5
56	23784.0
57	20827.0
58	17870.0
59	17386.5
60	16903.0
61	16903.0
62	16036.5
63	15170.0
64	11906.0
65	8642.0
66	6981.5
67	5321.0
68	5321.0
69	4241.0
70	3161.0
71	2508.0
72	1855.0
73	1471.0
74	1087.0
75	1087.0
76	812.0
77	537.0
78	412.0
79	287.0
80	221.5
81	156.0
82	156.0
83	149.5
84	143.0
85	117.5
86	92.0
87	71.0
88	50.0
89	50.0
90	37.0
91	24.0
92	13.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	365872.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.47046219335497
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.92217704044978	51.69302043448176
2	6.6563090000089575	7.9170754546464215
3	2.039909027386107	3.63942998073147
4	1.0679167435053076	2.540380092811326
5	0.6508076482638199	1.9351915820609884
6	0.4414052222252848	1.575034354817895
7	0.309564759392519	1.288697152389348
8	0.23931307837104063	1.1385647503712293
9	0.19494839808485465	1.0434304204164417
>10	1.2842361073455608	14.996403172525635
>50	0.12940580339506944	5.308738647762752
>100	0.06122425106862779	5.771591367857109
>500	0.002782920503119445	1.1524425891276526
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	790	0.21592250841824465	No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG	761	0.20799623912187867	No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG	680	0.18585734901823586	No Hit
CTTTAAAGCCTTAGGCCGTATGACAAAATGAAGAGACTGAAAT	670	0.18312415270914417	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	641	0.17519788341277825	No Hit
GTGTGGGGTCATGGAGCCCAATCCAGACGTATGAGTAGCTGTT	636	0.17383128525823238	No Hit
GATTATAACTGTAATGTGAGGTTACCCTATGTCTGCAAGTTCA	376	0.10276818122184808	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.733196309091704E-4	0.0
4	0.0	0.0	0.0	2.733196309091704E-4	0.0
5	0.0	0.0	0.0	2.733196309091704E-4	0.0
6	0.0	0.0	2.733196309091704E-4	2.733196309091704E-4	0.0
7	0.0	0.0	0.001913237416364193	2.733196309091704E-4	0.0
8	0.0	0.0	0.0024598766781825337	2.733196309091704E-4	0.0
9	0.0	0.0	0.0027331963090917043	5.466392618183408E-4	0.0
10	0.0	0.0	0.003279835570910045	8.199588927275112E-4	0.0
11	0.0	0.0	0.0049197533563650675	8.199588927275112E-4	0.0
12	0.0	0.0	0.0049197533563650675	8.199588927275112E-4	0.0
13	0.0	0.0	0.0049197533563650675	0.0010932785236366816	0.0
14	0.0	0.0	0.005193072987274238	0.0010932785236366816	0.0
15	0.0	0.0	0.005739712249092579	0.0010932785236366816	0.0
16	0.0	0.0	0.005739712249092579	0.0010932785236366816	0.0
17	0.0	0.0	0.006286351510910919	0.0010932785236366816	0.0
18	0.0	0.0	0.006286351510910919	0.0013665981545458521	0.0
19	0.0	0.0	0.00655967114182009	0.0016399177854550225	0.0
20	0.0	0.0	0.00655967114182009	0.0016399177854550225	0.0
21	0.0	0.0	0.0068329907727292605	0.0016399177854550225	0.0
22	0.0	0.0	0.007106310403638431	0.0016399177854550225	0.0
23	0.0	0.0	0.007379630034547602	0.001913237416364193	0.0
24	0.0	0.0	0.007379630034547602	0.002186557047273363	0.0
25	0.0	0.0	0.007379630034547602	0.0024598766781825337	0.0
26	0.0	0.0	0.007379630034547602	0.0030065159400008744	0.0
27	0.0	0.0	0.007379630034547602	0.0035531552018192155	0.0
28	0.0	0.0	0.007379630034547602	0.007379630034547602	0.0
29	0.0	0.0	0.007379630034547602	0.018585734901823588	0.0
30	0.0	0.0	0.007379630034547602	0.03826474832728386	0.0
31	0.0	0.0	0.007379630034547602	0.08445576595093365	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGGC	20	0.0018402966	37.0	7
ACGGGTA	25	0.0054915063	29.6	16
GGTATCA	185	0.0	26.0	1
GTATCAA	550	0.0	25.227274	1
AGCCTTA	140	0.0	21.142859	7
CCATCGG	45	0.0038209	20.555553	19
TACAACC	45	0.0038209	20.555553	4
AAGCCTT	160	0.0	19.65625	6
CAACGCA	770	0.0	19.22078	5
AAAGCCT	165	0.0	19.060606	5
AACGCAG	780	0.0	18.97436	6
GACGTAT	200	0.0	18.5	26
GCACCGC	70	1.2163165E-4	18.5	10
ATATAAG	60	9.2204165E-4	18.5	1
TCGTCTT	50	0.0070259403	18.5	14
TTTATAC	70	1.2163165E-4	18.5	3
CGTAAAC	50	0.0070259403	18.5	3
CCTCTAT	60	9.2204165E-4	18.5	1
ACGCAGA	805	0.0	18.385094	7
TCAACGC	805	0.0	18.385094	4
>>END_MODULE