FastQCFastQC Report
Fri 10 Feb 2017
ERR1632981.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632981.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257734
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4940.1916704819697828No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3860.14976681384683432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1550.031.0322591
GGGTCGC250.005488532629.59999813
GTATAAT400.001927146323.1251
GTATTAA652.669458E-622.769231
GGTACCG855.1415554E-821.7647066
GGCCGTT855.1415554E-821.76470614
CGTTAAA855.1415554E-821.76470617
GCCGTTA909.406904E-820.55555715
CCGTTAA909.406904E-820.55555716
GTGTCAC851.2376549E-619.58823627
GTATCAA3600.019.5277791
TCTCGTC801.6087544E-518.512
GTATAGA609.2103356E-418.51
TCATATA701.2145457E-418.52
TTTAGGT500.007020443718.4999983
CGAGGGT1002.853485E-718.49999816
ACATGTG1002.853485E-718.49999823
CGCGGCC1156.347909E-817.69565211
CGGCCGT1054.7616595E-717.61904713
GGGTTCA953.5844714E-617.52631619
Produced by FastQC (version 0.11.5)