##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632981.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	257734
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.86839920227832	33.0	33.0	33.0	27.0	33.0
2	30.6188706185447	33.0	33.0	33.0	27.0	33.0
3	31.077219148424344	33.0	33.0	33.0	27.0	33.0
4	31.223695748329675	33.0	33.0	33.0	27.0	33.0
5	31.360480184996934	33.0	33.0	33.0	27.0	33.0
6	33.85972359098916	37.0	33.0	37.0	27.0	37.0
7	34.09862105892121	37.0	33.0	37.0	27.0	37.0
8	34.240111898313764	37.0	33.0	37.0	27.0	37.0
9	34.33264528544934	37.0	37.0	37.0	27.0	37.0
10	34.410477469018446	37.0	37.0	37.0	27.0	37.0
11	34.410236910923665	37.0	37.0	37.0	27.0	37.0
12	34.377125253167996	37.0	37.0	37.0	27.0	37.0
13	34.413232247200604	37.0	37.0	37.0	27.0	37.0
14	34.3819752147563	37.0	37.0	37.0	27.0	37.0
15	34.40198809625428	37.0	37.0	37.0	27.0	37.0
16	34.38504815041865	37.0	37.0	37.0	27.0	37.0
17	34.38212653355785	37.0	37.0	37.0	27.0	37.0
18	34.380698704866255	37.0	37.0	37.0	27.0	37.0
19	34.3645425128233	37.0	37.0	37.0	27.0	37.0
20	34.36266460769631	37.0	37.0	37.0	27.0	37.0
21	34.37866948093771	37.0	37.0	37.0	27.0	37.0
22	34.39237353240162	37.0	37.0	37.0	27.0	37.0
23	34.37759860941901	37.0	37.0	37.0	27.0	37.0
24	34.34938735285217	37.0	37.0	37.0	27.0	37.0
25	34.33849627910947	37.0	37.0	37.0	27.0	37.0
26	34.29913011088952	37.0	37.0	37.0	27.0	37.0
27	34.23206484204645	37.0	37.0	37.0	27.0	37.0
28	34.190514251127134	37.0	37.0	37.0	27.0	37.0
29	34.240387376131984	37.0	37.0	37.0	27.0	37.0
30	34.17838934715637	37.0	37.0	37.0	27.0	37.0
31	34.189501579147496	37.0	37.0	37.0	27.0	37.0
32	34.20663940341593	37.0	37.0	37.0	27.0	37.0
33	34.16258623231704	37.0	37.0	37.0	27.0	37.0
34	34.10805714418742	37.0	37.0	37.0	27.0	37.0
35	34.09601371957134	37.0	37.0	37.0	27.0	37.0
36	34.07277658360946	37.0	37.0	37.0	27.0	37.0
37	34.09668107428589	37.0	37.0	37.0	27.0	37.0
38	33.953715846570496	37.0	33.0	37.0	27.0	37.0
39	34.049310529460605	37.0	33.0	37.0	27.0	37.0
40	34.084591090038565	37.0	33.0	37.0	27.0	37.0
41	34.10382021774387	37.0	37.0	37.0	27.0	37.0
42	34.01156230842652	37.0	33.0	37.0	27.0	37.0
43	33.72072369186836	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	10.0
16	17.0
17	15.0
18	20.0
19	27.0
20	71.0
21	182.0
22	513.0
23	999.0
24	1759.0
25	2911.0
26	4176.0
27	5732.0
28	7468.0
29	9319.0
30	11672.0
31	13936.0
32	16959.0
33	21235.0
34	29112.0
35	48034.0
36	83566.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.01839105434285	21.50356569175972	13.225651252842077	25.25239200105535
2	16.503837289608665	23.39388672041717	36.48373904878673	23.618536941187426
3	18.126828435518792	27.157068916014186	29.796612010832874	24.91949063763415
4	13.37891003903249	18.749951500384118	38.17385366307899	29.697284797504402
5	14.674043781573252	37.23063313338558	35.1893813001001	12.905941784941064
6	30.085669721495805	39.84767240643454	17.291471051549273	12.775186820520382
7	26.81136365399986	32.08734586822072	23.795851536855828	17.305438940923587
8	24.46863820838539	36.65872566289275	20.417950289833705	18.454685838888157
9	25.495278077397625	15.644424096161158	20.207267958437768	38.65302986800344
10	14.433097689866296	28.633009226566923	34.43821924930355	22.495673834263233
11	34.27642453071771	23.564605368325484	24.108965056996748	18.050005043960052
12	22.60819294311189	25.94186254044868	30.383263364554153	21.066681151885277
13	27.62732119161616	22.257055724118665	26.227040281840967	23.888582802424203
14	21.745287777320804	20.897902488612292	27.3766751767326	29.980134557334303
15	23.49282593681858	29.430342911684136	23.866854974508602	23.20997617698868
16	23.158372585689122	28.851839493431214	25.004073967734175	22.985713953145492
17	22.51080571441874	27.90202301597771	26.507562060108487	23.07960920949506
18	22.836723133152788	27.85856736014651	27.445738629750053	21.858970876950654
19	24.231184089022015	27.066665632008196	26.88391907936089	21.8182311996089
20	23.641040762957157	27.065113644299938	27.221476405906863	22.072369186836042
21	23.02334965507073	27.36542326584773	27.2210884089798	22.390138670101734
22	22.639232697277038	27.277735960331196	27.147756989764645	22.93527435262713
23	22.718384070398162	26.919226799723745	27.68707271838407	22.67531641149402
24	22.98028199616659	27.768940069994645	26.126549077731305	23.12422885610746
25	23.157208594907928	27.232728316791732	27.327787563922495	22.282275524377848
26	22.92829040793997	27.41974283563674	27.044161810238464	22.607804946184825
27	22.839051114715172	27.41004291246013	26.47846229057866	23.272443682246035
28	22.588017102904544	27.191988639449978	27.509758122715667	22.71023613492981
29	23.788867592168668	27.088781456850864	26.68914462197459	22.433206329005877
30	23.10560500360837	26.92039079050494	27.182288716273366	22.791715489613324
31	23.241791925007956	27.04687778872791	27.215656452000903	22.495673834263233
32	22.527101585355442	26.965786430971466	27.141937035858675	23.365174947814413
33	22.539905483948566	27.334771508609652	27.436038706573445	22.689284300868337
34	23.152164634856092	26.572357546928227	27.30140377288212	22.974074045333563
35	22.842931083985814	27.475614393134006	27.251352169290822	22.43010235358936
36	23.634444815197064	27.04454980716553	26.5552856821374	22.76571969550001
37	22.836723133152788	26.865683223788867	26.96772641560679	23.329867227451558
38	22.993085894759712	27.125641164921976	26.972770375658623	22.90850256465969
39	22.60625295847657	26.973158372585686	27.27812395725826	23.142464711679484
40	22.808011360550022	27.437202697354635	27.18422870090869	22.570557241186652
41	22.81887527450783	26.636377039893844	27.255232138561464	23.289515547036867
42	22.514685683689383	27.52217402438173	27.247084203093113	22.716056088835774
43	23.179324419750596	26.343051363033204	27.40111898313765	23.076505234078546
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	6.5
2	9.0
3	28.0
4	47.0
5	47.0
6	72.5
7	98.0
8	97.0
9	96.0
10	117.5
11	139.0
12	139.0
13	227.0
14	315.0
15	553.5
16	792.0
17	954.5
18	1117.0
19	1117.0
20	1196.5
21	1276.0
22	1592.5
23	1909.0
24	2618.0
25	3327.0
26	3327.0
27	4395.5
28	5464.0
29	6699.5
30	7935.0
31	9399.5
32	10864.0
33	10864.0
34	12344.5
35	13825.0
36	15347.5
37	16870.0
38	18280.0
39	19690.0
40	19690.0
41	20484.0
42	21278.0
43	21528.5
44	21779.0
45	21255.5
46	20732.0
47	20732.0
48	19740.0
49	18748.0
50	18593.5
51	18439.0
52	17326.5
53	16214.0
54	16214.0
55	15461.5
56	14709.0
57	12928.0
58	11147.0
59	10385.0
60	9623.0
61	9623.0
62	8519.5
63	7416.0
64	6230.0
65	5044.0
66	4167.5
67	3291.0
68	3291.0
69	2753.0
70	2215.0
71	1793.0
72	1371.0
73	1088.0
74	805.0
75	805.0
76	630.5
77	456.0
78	364.5
79	273.0
80	221.5
81	170.0
82	170.0
83	132.0
84	94.0
85	96.0
86	98.0
87	65.5
88	33.0
89	33.0
90	25.0
91	17.0
92	11.0
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	257734.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.29811147286499
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.26447769120055	65.25951270158352
2	5.393563722296609	7.798889424612017
3	1.4976898502325708	3.248404432316786
4	0.758655550662552	2.193974542852358
5	0.42808964025575863	1.5475036265794753
6	0.30780519549946606	1.3352240601688439
7	0.2185685119025833	1.1061463446593829
8	0.15698988266997776	0.9080057629908838
9	0.11430202610414808	0.7437438562366818
>10	0.7854042506828567	10.555896921751803
>50	0.049278693678774625	2.3634242229674225
>100	0.025174984814156604	2.9392741032808023
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	494	0.1916704819697828	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	386	0.14976681384683432	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.8799692706433767E-4	0.0
5	0.0	0.0	0.0	3.8799692706433767E-4	0.0
6	0.0	0.0	3.8799692706433767E-4	3.8799692706433767E-4	0.0
7	0.0	0.0	0.0015519877082573507	3.8799692706433767E-4	0.0
8	0.0	0.0	0.0015519877082573507	3.8799692706433767E-4	0.0
9	0.0	0.0	0.002327981562386026	0.001163990781193013	0.0
10	3.8799692706433767E-4	0.0	0.002327981562386026	0.001163990781193013	0.0
11	3.8799692706433767E-4	0.0	0.0031039754165147013	0.001163990781193013	0.0
12	3.8799692706433767E-4	0.0	0.003491972343579039	0.001163990781193013	0.0
13	3.8799692706433767E-4	0.0	0.0038799692706433767	0.001163990781193013	0.0
14	3.8799692706433767E-4	0.0	0.0042679661977077146	0.001163990781193013	0.0
15	3.8799692706433767E-4	0.0	0.004655963124772052	0.001163990781193013	0.0
16	3.8799692706433767E-4	0.0	0.004655963124772052	0.001163990781193013	3.8799692706433767E-4
17	3.8799692706433767E-4	0.0	0.004655963124772052	0.0015519877082573507	3.8799692706433767E-4
18	3.8799692706433767E-4	0.0	0.0050439600518363895	0.0015519877082573507	3.8799692706433767E-4
19	3.8799692706433767E-4	0.0	0.0050439600518363895	0.0015519877082573507	3.8799692706433767E-4
20	3.8799692706433767E-4	0.0	0.0050439600518363895	0.0015519877082573507	3.8799692706433767E-4
21	3.8799692706433767E-4	0.0	0.0050439600518363895	0.0015519877082573507	0.001163990781193013
22	3.8799692706433767E-4	0.0	0.0050439600518363895	0.0019399846353216883	0.001163990781193013
23	3.8799692706433767E-4	0.0	0.0050439600518363895	0.0019399846353216883	0.001163990781193013
24	3.8799692706433767E-4	0.0	0.0050439600518363895	0.0031039754165147013	0.001163990781193013
25	3.8799692706433767E-4	0.0	0.005431956978900727	0.0031039754165147013	0.001163990781193013
26	3.8799692706433767E-4	0.0	0.005819953905965064	0.0031039754165147013	0.001163990781193013
27	3.8799692706433767E-4	0.0	0.005819953905965064	0.003491972343579039	0.001163990781193013
28	3.8799692706433767E-4	0.0	0.006207950833029403	0.006207950833029403	0.001163990781193013
29	3.8799692706433767E-4	0.0	0.006207950833029403	0.015131880155509168	0.001163990781193013
30	3.8799692706433767E-4	0.0	0.006207950833029403	0.02793577874863231	0.001163990781193013
31	3.8799692706433767E-4	0.0	0.006207950833029403	0.07488340692341716	0.001163990781193013
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	155	0.0	31.032259	1
GGGTCGC	25	0.0054885326	29.599998	13
GTATAAT	40	0.0019271463	23.125	1
GTATTAA	65	2.669458E-6	22.76923	1
GGTACCG	85	5.1415554E-8	21.764706	6
GGCCGTT	85	5.1415554E-8	21.764706	14
CGTTAAA	85	5.1415554E-8	21.764706	17
GCCGTTA	90	9.406904E-8	20.555557	15
CCGTTAA	90	9.406904E-8	20.555557	16
GTGTCAC	85	1.2376549E-6	19.588236	27
GTATCAA	360	0.0	19.527779	1
TCTCGTC	80	1.6087544E-5	18.5	12
GTATAGA	60	9.2103356E-4	18.5	1
TCATATA	70	1.2145457E-4	18.5	2
TTTAGGT	50	0.0070204437	18.499998	3
CGAGGGT	100	2.853485E-7	18.499998	16
ACATGTG	100	2.853485E-7	18.499998	23
CGCGGCC	115	6.347909E-8	17.695652	11
CGGCCGT	105	4.7616595E-7	17.619047	13
GGGTTCA	95	3.5844714E-6	17.526316	19
>>END_MODULE