FastQCFastQC Report
Fri 10 Feb 2017
ERR1632980.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632980.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438897
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16790.38254989211591783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12480.2843491753190384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6170.1405796804261592No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA4420.10070699959215942No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC4410.10047915570167944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGACC509.074378E-625.8999984
AACGACT659.3641574E-825.61538534
GGTATCA3250.023.9076921
GACTCCG701.9172876E-723.78571537
GTATCAA8500.022.4176451
CAACGAC753.7298742E-722.233
ATTGGAC753.7298742E-722.23
CCAACGA753.7298742E-722.232
ACGACTC753.7298742E-722.235
CCTAAAG450.003822096620.5555552
CTTACCC555.1361346E-420.1818183
CGGGTAA656.889708E-519.92307723
GGTACCG851.2417222E-619.5882366
GCCAACG851.2417222E-619.58823631
GCCGGCA609.22442E-418.515
TGCGGGT609.22442E-418.521
ACGTCAA609.22442E-418.529
CGACTCC902.1460528E-618.536
CGTCAAT609.22442E-418.530
TTCTGCG609.22442E-418.518
Produced by FastQC (version 0.11.5)