##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632980.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	438897
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.676719139114645	33.0	33.0	33.0	27.0	33.0
2	31.194154892833627	33.0	33.0	33.0	27.0	33.0
3	31.654283351219078	33.0	33.0	33.0	27.0	33.0
4	31.783883234563007	33.0	33.0	33.0	27.0	33.0
5	31.8989421208165	33.0	33.0	33.0	33.0	33.0
6	34.76798428788531	37.0	37.0	37.0	27.0	37.0
7	34.94224840908004	37.0	37.0	37.0	27.0	37.0
8	35.11841958363807	37.0	37.0	37.0	33.0	37.0
9	35.19270124881236	37.0	37.0	37.0	33.0	37.0
10	35.22511887754986	37.0	37.0	37.0	33.0	37.0
11	35.26467257693719	37.0	37.0	37.0	33.0	37.0
12	35.221439198718606	37.0	37.0	37.0	33.0	37.0
13	35.247511375106235	37.0	37.0	37.0	33.0	37.0
14	35.22732896328751	37.0	37.0	37.0	33.0	37.0
15	35.2399948051593	37.0	37.0	37.0	33.0	37.0
16	35.23616702779923	37.0	37.0	37.0	33.0	37.0
17	35.20598682606625	37.0	37.0	37.0	33.0	37.0
18	35.21158495045535	37.0	37.0	37.0	33.0	37.0
19	35.218338243369175	37.0	37.0	37.0	33.0	37.0
20	35.22444673807294	37.0	37.0	37.0	33.0	37.0
21	35.226182908518396	37.0	37.0	37.0	33.0	37.0
22	35.217265098645015	37.0	37.0	37.0	33.0	37.0
23	35.210475350708705	37.0	37.0	37.0	33.0	37.0
24	35.201578046785464	37.0	37.0	37.0	33.0	37.0
25	35.20209069553904	37.0	37.0	37.0	33.0	37.0
26	35.15537358423502	37.0	37.0	37.0	33.0	37.0
27	35.10736687651089	37.0	37.0	37.0	33.0	37.0
28	35.072495369072925	37.0	37.0	37.0	33.0	37.0
29	35.1238422682315	37.0	37.0	37.0	33.0	37.0
30	35.06407653731969	37.0	37.0	37.0	27.0	37.0
31	35.06452766822284	37.0	37.0	37.0	27.0	37.0
32	35.09062262899951	37.0	37.0	37.0	33.0	37.0
33	35.065147403604946	37.0	37.0	37.0	33.0	37.0
34	34.99991797619943	37.0	37.0	37.0	27.0	37.0
35	35.000997956240305	37.0	37.0	37.0	27.0	37.0
36	34.980232264061954	37.0	37.0	37.0	27.0	37.0
37	35.000881755856156	37.0	37.0	37.0	27.0	37.0
38	34.86030663230781	37.0	37.0	37.0	27.0	37.0
39	34.91559978764949	37.0	37.0	37.0	27.0	37.0
40	34.95260619234126	37.0	37.0	37.0	27.0	37.0
41	34.974615912161624	37.0	37.0	37.0	27.0	37.0
42	34.87106086393847	37.0	37.0	37.0	27.0	37.0
43	34.65186592754109	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	11.0
16	23.0
17	27.0
18	18.0
19	18.0
20	23.0
21	114.0
22	279.0
23	634.0
24	1258.0
25	2303.0
26	3736.0
27	5668.0
28	7949.0
29	10647.0
30	13920.0
31	17357.0
32	22268.0
33	28644.0
34	40751.0
35	73680.0
36	209569.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.47213810985265	20.97690346482204	13.776352993982643	24.77460543134266
2	15.575864040993673	23.640398544533227	38.24724251931546	22.53649489515763
3	17.530308933531103	27.215952717835844	31.14717120417775	24.106567144455305
4	12.712094181550567	18.2901683082819	39.1866428797645	29.811094630403034
5	13.485852033620644	37.63662089282907	35.62612640323356	13.251400670316727
6	29.03710893444248	40.023057801716575	17.7011918513911	13.238641412449844
7	25.521705548226574	32.944859500064936	24.003809549848825	17.52962540185966
8	23.999024828148745	36.43292161942323	20.992396849374686	18.575656703053337
9	25.860509413370337	15.654242339318792	20.265346994853005	38.219901252457866
10	14.45715053873688	28.70741882491792	34.62999291405501	22.205437722290196
11	33.18341205339749	24.081732160392985	24.5337744391053	18.201081347104218
12	22.082629865321476	26.169921416642172	30.774646443242947	20.972802274793402
13	27.593262200470726	22.554266718615075	26.85687074643937	22.99560033447483
14	21.71078863605812	21.463578014887318	28.05441823480224	28.771215114252318
15	23.40366874232451	29.14419556296808	25.018626238046735	22.433509456660676
16	22.573405605415395	29.120271954467675	25.369050141604976	22.937272298511953
17	21.898759845704117	28.100442700679203	26.71173418820361	23.28906326541307
18	22.294296839577395	27.865991337375284	28.053051171459366	21.786660651587958
19	24.205451392923624	26.905857182892568	28.010672207830083	20.878019216353724
20	23.80945871126939	26.53948420700073	28.200921856380884	21.450135225348998
21	22.281081893929557	27.908370301004563	27.48480850860225	22.325739296463635
22	22.189032962175638	27.50622583430737	27.623793281795045	22.680947921721952
23	21.95298669163836	27.684399756662724	28.08882266226472	22.273790889434196
24	22.866640692463154	27.844574011670165	27.012943811418168	22.275841484448517
25	22.86276734632499	27.19180126544497	27.90426911097592	22.041162277254116
26	22.642897992011793	27.495972859235767	27.575262533122807	22.285866615629637
27	22.477483327523316	27.330102506966327	27.274508597689206	22.91790556782115
28	22.02133985878236	27.56022483635113	28.04416525973064	22.374270045135873
29	23.11180071861963	27.601236736637524	27.257420305903207	22.029542238839635
30	22.576367575991636	27.550883236841443	27.721993998591927	22.150755188574998
31	22.83086920165779	27.253774803655528	27.803334267493284	22.112021727193394
32	21.84020396585076	27.16605490582073	28.092240320621926	22.901500807706594
33	22.017466512644194	27.052588648361684	28.35289373133104	22.577051107663074
34	22.422117262136673	27.09109426585281	27.838650070517684	22.648138401492833
35	22.482723737004353	27.733158349225445	27.73612031980168	22.047997593968518
36	22.83269195278163	27.52012431162665	27.356304554371526	22.290879181220195
37	22.92793069900227	26.768695160823608	27.581186474275288	22.722187665898833
38	22.870741882491792	27.29501454783241	27.426480472639366	22.407763097036433
39	22.307967473006197	27.11342296711985	27.974217185353282	22.604392374520675
40	22.243943339781318	27.507365053759763	27.96214145915784	22.286550147301078
41	22.113160946645795	26.973298974474645	28.116619616903282	22.79692046197627
42	22.417788228217557	27.28977413835137	27.657514177586084	22.634923455844994
43	22.472698605823236	26.61717897365441	27.847763826136884	23.062358594385472
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	18.0
2	26.0
3	96.5
4	167.0
5	167.0
6	224.0
7	281.0
8	263.0
9	245.0
10	372.5
11	500.0
12	500.0
13	728.0
14	956.0
15	1768.0
16	2580.0
17	2954.0
18	3328.0
19	3328.0
20	3199.0
21	3070.0
22	3710.0
23	4350.0
24	5625.5
25	6901.0
26	6901.0
27	8656.0
28	10411.0
29	13330.5
30	16250.0
31	17821.5
32	19393.0
33	19393.0
34	21782.0
35	24171.0
36	26149.5
37	28128.0
38	30080.5
39	32033.0
40	32033.0
41	33792.5
42	35552.0
43	34463.5
44	33375.0
45	33413.5
46	33452.0
47	33452.0
48	32460.0
49	31468.0
50	31958.0
51	32448.0
52	31932.5
53	31417.0
54	31417.0
55	27804.5
56	24192.0
57	21312.0
58	18432.0
59	16354.5
60	14277.0
61	14277.0
62	12416.0
63	10555.0
64	8789.5
65	7024.0
66	6078.5
67	5133.0
68	5133.0
69	4243.5
70	3354.0
71	2823.0
72	2292.0
73	1860.0
74	1428.0
75	1428.0
76	1100.5
77	773.0
78	597.0
79	421.0
80	311.5
81	202.0
82	202.0
83	164.5
84	127.0
85	106.0
86	85.0
87	71.5
88	58.0
89	58.0
90	42.0
91	26.0
92	16.0
93	6.0
94	3.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	438897.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.28396063823914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.88041016872552	56.87988339266414
2	5.49923153588701	6.960263041152738
3	1.4557577750558803	2.763783532063407
4	0.6833526510175109	1.7298104907611407
5	0.4401525488647058	1.3927298288587333
6	0.2901497775253438	1.101709626006462
7	0.20173845031693044	0.8936765704353191
8	0.1583933849014335	0.8019008588367823
9	0.14269691617099894	0.8127383423547246
>10	1.0405174309445955	13.252015706857176
>50	0.14807786560425257	6.382124846501215
>100	0.058432687150695085	6.215376990828074
>500	3.629359450353732E-4	0.1417127084490936
>1k	7.258718900707464E-4	0.6722740642309514
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1679	0.38254989211591783	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1248	0.2843491753190384	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	617	0.1405796804261592	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	442	0.10070699959215942	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	441	0.10047915570167944	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.2784389047999872E-4	0.0	0.0
2	0.0	0.0	2.2784389047999872E-4	0.0	0.0
3	0.0	0.0	4.5568778095999744E-4	0.0	0.0
4	0.0	0.0	6.835316714399962E-4	2.2784389047999872E-4	0.0
5	0.0	0.0	0.0011392194523999937	2.2784389047999872E-4	0.0
6	0.0	0.0	0.0013670633428799924	2.2784389047999872E-4	0.0
7	0.0	0.0	0.003189814466719982	4.5568778095999744E-4	0.0
8	0.0	0.0	0.005012565590559972	4.5568778095999744E-4	0.0
9	0.0	0.0	0.006607472823919963	4.5568778095999744E-4	0.0
10	0.0011392194523999937	0.0	0.00706316060487996	6.835316714399962E-4	0.0
11	0.0011392194523999937	0.0	0.011847882304959933	9.113755619199949E-4	0.0
12	0.0011392194523999937	0.0	0.012759257866879928	9.113755619199949E-4	0.0
13	0.0011392194523999937	0.0	0.013670633428799924	9.113755619199949E-4	0.0
14	0.0011392194523999937	0.0	0.015265540662159915	0.0011392194523999937	0.0
15	0.0011392194523999937	0.0	0.0175439795669599	0.0011392194523999937	0.0
16	0.0011392194523999937	0.0	0.020278106252719887	0.0011392194523999937	0.0
17	0.0011392194523999937	0.0	0.020961637924159882	0.0011392194523999937	0.0
18	0.0011392194523999937	0.0	0.021645169595599877	0.0011392194523999937	0.0
19	0.0011392194523999937	0.0	0.022328701267039876	0.0011392194523999937	0.0
20	0.0011392194523999937	0.0	0.022784389047999874	0.0011392194523999937	0.0
21	0.0011392194523999937	0.0	0.023923608500399866	0.0013670633428799924	0.0
22	0.0011392194523999937	0.0	0.023923608500399866	0.001594907233359991	0.0
23	0.0011392194523999937	2.2784389047999872E-4	0.024834984062319862	0.0020505950143199884	0.0
24	0.0011392194523999937	2.2784389047999872E-4	0.024834984062319862	0.0022784389047999874	0.0
25	0.0011392194523999937	2.2784389047999872E-4	0.024834984062319862	0.0022784389047999874	0.0
26	0.0011392194523999937	2.2784389047999872E-4	0.024834984062319862	0.002506282795279986	0.0
27	0.0011392194523999937	2.2784389047999872E-4	0.024834984062319862	0.0038733461381599784	0.0
28	0.0011392194523999937	2.2784389047999872E-4	0.024834984062319862	0.010025131181119943	0.0
29	0.0011392194523999937	2.2784389047999872E-4	0.024834984062319862	0.02460714017183986	0.0
30	0.0011392194523999937	2.2784389047999872E-4	0.024834984062319862	0.04739152921983973	0.0
31	0.0011392194523999937	2.2784389047999872E-4	0.024834984062319862	0.11711175970671935	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGGACC	50	9.074378E-6	25.899998	4
AACGACT	65	9.3641574E-8	25.615385	34
GGTATCA	325	0.0	23.907692	1
GACTCCG	70	1.9172876E-7	23.785715	37
GTATCAA	850	0.0	22.417645	1
CAACGAC	75	3.7298742E-7	22.2	33
ATTGGAC	75	3.7298742E-7	22.2	3
CCAACGA	75	3.7298742E-7	22.2	32
ACGACTC	75	3.7298742E-7	22.2	35
CCTAAAG	45	0.0038220966	20.555555	2
CTTACCC	55	5.1361346E-4	20.181818	3
CGGGTAA	65	6.889708E-5	19.923077	23
GGTACCG	85	1.2417222E-6	19.588236	6
GCCAACG	85	1.2417222E-6	19.588236	31
GCCGGCA	60	9.22442E-4	18.5	15
TGCGGGT	60	9.22442E-4	18.5	21
ACGTCAA	60	9.22442E-4	18.5	29
CGACTCC	90	2.1460528E-6	18.5	36
CGTCAAT	60	9.22442E-4	18.5	30
TTCTGCG	60	9.22442E-4	18.5	18
>>END_MODULE