FastQCFastQC Report
Fri 10 Feb 2017
ERR1632979.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632979.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374128
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8910.23815378693922934No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT8420.22505666509857591No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG7410.19806055681477996No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC7400.19779326861395033No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC6350.16972800752683573No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC6100.16304580250609418No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6090.16277851430526452No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA5620.15021596886627037No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT5520.14754308685797374No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA5040.13471325321814995No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT4670.12482358978745242No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA4410.1178740965658812No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC4390.11733952016422187No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC4120.11012273874182099No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA4030.10771714493435403No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA3830.10237138091776077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1800.026.722221
GTGTGAT400.001928766123.12511
GTATCAA5450.023.082571
TCGCCAT1400.021.14285713
CTAACCG705.0867584E-621.1428576
TAACCGT806.934315E-720.81257
GTGTTAC450.00382105920.5555531
GACAGTC450.00382105920.5555537
GCTCTAG555.134187E-420.1818181
ACAGATC555.134187E-420.1818188
AGGGGTT555.134187E-420.1818185
CGTGCAA759.238403E-619.73333411
GTCGCCA1551.8189894E-1219.09677512
AACCGTG801.6119253E-518.58
GTACCCT801.6119253E-518.51
CTATAGG609.22095E-418.54
CCTCGGC1103.8309736E-818.521
TAGATTG609.22095E-418.515
GTATAGG1103.8309736E-818.51
TCTATAG701.21640995E-418.53
Produced by FastQC (version 0.11.5)