##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632979.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	374128
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.461724329641193	33.0	33.0	33.0	27.0	33.0
2	31.047518496343496	33.0	33.0	33.0	27.0	33.0
3	31.48881131591327	33.0	33.0	33.0	27.0	33.0
4	31.64160127015353	33.0	33.0	33.0	27.0	33.0
5	31.760205063507676	33.0	33.0	33.0	27.0	33.0
6	34.58136252833255	37.0	37.0	37.0	27.0	37.0
7	34.76255452679297	37.0	37.0	37.0	27.0	37.0
8	34.90892689133131	37.0	37.0	37.0	27.0	37.0
9	35.014257152632254	37.0	37.0	37.0	27.0	37.0
10	35.04530267715862	37.0	37.0	37.0	27.0	37.0
11	35.07483535046829	37.0	37.0	37.0	33.0	37.0
12	35.02178131548561	37.0	37.0	37.0	27.0	37.0
13	35.05797481075995	37.0	37.0	37.0	27.0	37.0
14	35.04166488474533	37.0	37.0	37.0	27.0	37.0
15	35.07475516400804	37.0	37.0	37.0	33.0	37.0
16	35.04622214856948	37.0	37.0	37.0	27.0	37.0
17	35.00578946242997	37.0	37.0	37.0	27.0	37.0
18	35.000483791643504	37.0	37.0	37.0	27.0	37.0
19	35.02363094983535	37.0	37.0	37.0	27.0	37.0
20	35.026068618226915	37.0	37.0	37.0	27.0	37.0
21	35.023590856605225	37.0	37.0	37.0	27.0	37.0
22	35.03410330154386	37.0	37.0	37.0	27.0	37.0
23	35.02924667493478	37.0	37.0	37.0	27.0	37.0
24	35.00144335628448	37.0	37.0	37.0	27.0	37.0
25	35.003739361929604	37.0	37.0	37.0	27.0	37.0
26	34.95373508531839	37.0	37.0	37.0	27.0	37.0
27	34.914189796005644	37.0	37.0	37.0	27.0	37.0
28	34.879196424753026	37.0	37.0	37.0	27.0	37.0
29	34.91828465124235	37.0	37.0	37.0	27.0	37.0
30	34.8836654834709	37.0	37.0	37.0	27.0	37.0
31	34.86624631142283	37.0	37.0	37.0	27.0	37.0
32	34.89038778172176	37.0	37.0	37.0	27.0	37.0
33	34.86870001710645	37.0	37.0	37.0	27.0	37.0
34	34.812347645725524	37.0	37.0	37.0	27.0	37.0
35	34.80642721207715	37.0	37.0	37.0	27.0	37.0
36	34.78376919129282	37.0	37.0	37.0	27.0	37.0
37	34.81764797074798	37.0	37.0	37.0	27.0	37.0
38	34.66975740922893	37.0	37.0	37.0	27.0	37.0
39	34.7364992729761	37.0	37.0	37.0	27.0	37.0
40	34.775256062096396	37.0	37.0	37.0	27.0	37.0
41	34.791237223624	37.0	37.0	37.0	27.0	37.0
42	34.71402835393234	37.0	37.0	37.0	27.0	37.0
43	34.4842460334431	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	9.0
16	19.0
17	22.0
18	19.0
19	21.0
20	29.0
21	102.0
22	318.0
23	713.0
24	1442.0
25	2419.0
26	3958.0
27	5694.0
28	7730.0
29	10144.0
30	12918.0
31	16298.0
32	20206.0
33	25694.0
34	35986.0
35	63333.0
36	167052.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.29075610486251	20.990676987555062	13.927586280631227	24.790980626951203
2	15.434289868708037	23.501315057948084	38.60790959243895	22.45648548090493
3	17.596384125219174	27.115318821365953	31.522099388444598	23.766197664970278
4	13.025221314630286	18.223976820767223	38.74128640465295	30.009515459949537
5	13.290371209853314	38.0046401231664	35.823568404396354	12.881420262583928
6	29.192950006414918	39.78771971090108	17.78455501860326	13.234775264080742
7	25.495285036137368	32.83207886071077	24.237961339434634	17.434674763717233
8	23.33613094983535	37.658234614891164	20.98452935893598	18.02110507633751
9	25.161709361501945	16.350821109352946	20.322723773681734	38.16474575546337
10	14.056151905230296	29.350115468502757	34.71966813496985	21.874064491297098
11	32.73211307360048	24.32028610529017	24.616441431809434	18.33115938929992
12	21.652482572809305	26.352478296198097	30.639513749305053	21.355525381687553
13	26.829855022879872	22.7427511439935	27.397575161442074	23.029818671684556
14	21.445334217166316	21.60116323825001	27.782202882435957	29.171299662147714
15	23.40081469443613	29.265652397040586	25.086601377068813	22.246931531454475
16	22.81224607620921	29.269929008253857	25.06414916819912	22.85367574733781
17	22.019736560749262	28.54103408459137	26.49948680665441	22.93974254800496
18	21.644731214985246	28.551992900825386	28.02463328058846	21.778642603600908
19	24.108593850233078	27.348126844288583	27.88510883975538	20.65817046572296
20	23.628544241543	26.82263824145747	28.259846897318564	21.288970619680963
21	21.893041953556004	28.365158448445456	27.25110122738742	22.490698370611128
22	21.74469700209554	28.032919214814182	27.243617157764188	22.97876662532609
23	21.774900568789292	28.290050464012317	27.752801180344694	22.182247786853697
24	23.267705170422957	28.282833682589914	26.565774280460165	21.883686866526965
25	23.11561818415088	27.40746482487277	27.42670957533251	22.050207415643843
26	22.730188598554506	27.699343540178763	27.21020613266048	22.36026172860625
27	22.421203438395416	27.494868066544072	27.10810203994355	22.975826455116966
28	21.628961211136296	27.890721891972802	28.11070008125561	22.36961681563529
29	22.94081170080828	28.03452294401916	27.001454047812512	22.023211307360047
30	22.16300303639396	28.158544669204122	27.413345165291027	22.26510712911089
31	22.67753282299106	27.68303895992815	27.556077064534062	22.083351152546722
32	21.590738998417656	27.778995424026004	27.76563101398452	22.864634563571826
33	22.060898943677028	27.4005153316512	27.967968182012577	22.570617542659196
34	22.111950990035496	27.520260445622892	27.810000855322244	22.557787709019372
35	22.593604327930546	28.013941752555276	27.109973057349357	22.28248086216482
36	22.651605867510586	28.142774665355176	27.024708121284696	22.180911345849548
37	23.13138818799983	26.86700808279519	27.13189068981739	22.86971303938759
38	23.140475986828037	27.607930975495016	27.240409699354235	22.011183338322713
39	22.4345678484369	27.541376213488434	27.710035068211948	22.314020869862723
40	22.432429542830263	27.27061326604798	27.75146473934055	22.545492451781207
41	21.978574177821493	27.539772484283453	28.066062951717058	22.415590386177993
42	22.681542146003505	27.16316340931446	27.436599238763204	22.71869520591883
43	22.36293461061455	26.867275370996026	27.77953000042766	22.990260017961766
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	9.5
2	16.0
3	53.5
4	91.0
5	91.0
6	124.0
7	157.0
8	148.0
9	139.0
10	201.0
11	263.0
12	263.0
13	391.0
14	519.0
15	1063.5
16	1608.0
17	1845.0
18	2082.0
19	2082.0
20	2181.0
21	2280.0
22	2944.0
23	3608.0
24	4709.5
25	5811.0
26	5811.0
27	7258.5
28	8706.0
29	11765.0
30	14824.0
31	15987.0
32	17150.0
33	17150.0
34	19156.5
35	21163.0
36	22629.0
37	24095.0
38	26552.5
39	29010.0
40	29010.0
41	30889.5
42	32769.0
43	30608.5
44	28448.0
45	28544.5
46	28641.0
47	28641.0
48	28130.0
49	27619.0
50	28161.5
51	28704.0
52	28372.0
53	28040.0
54	28040.0
55	23673.0
56	19306.0
57	16830.0
58	14354.0
59	12499.5
60	10645.0
61	10645.0
62	9405.0
63	8165.0
64	6790.0
65	5415.0
66	4584.5
67	3754.0
68	3754.0
69	3075.0
70	2396.0
71	2118.0
72	1840.0
73	1473.5
74	1107.0
75	1107.0
76	860.0
77	613.0
78	493.5
79	374.0
80	269.5
81	165.0
82	165.0
83	135.5
84	106.0
85	96.0
86	86.0
87	61.0
88	36.0
89	36.0
90	26.0
91	16.0
92	10.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	374128.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.31322421039364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.3066890120282	54.4668758208075
2	5.194961546419991	6.2664976102720455
3	1.3610053307663497	2.462598589981554
4	0.7006531233026639	1.6903459567786463
5	0.40691669712035705	1.227122899418647
6	0.27532948020145903	0.9963605202673042
7	0.20917511362365304	0.8831217869052568
8	0.15630093067271958	0.7541610460765544
9	0.11979152677280805	0.650251189147837
>10	1.0235819545709535	12.832340202180037
>50	0.14825024447444218	6.305876981455275
>100	0.09287967123698378	9.663768593514522
>500	0.004465368809470374	1.8006788031948635
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	891	0.23815378693922934	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	842	0.22505666509857591	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	741	0.19806055681477996	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	740	0.19779326861395033	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	635	0.16972800752683573	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	610	0.16304580250609418	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	609	0.16277851430526452	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	562	0.15021596886627037	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	552	0.14754308685797374	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	504	0.13471325321814995	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	467	0.12482358978745242	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	441	0.1178740965658812	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	439	0.11733952016422187	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	412	0.11012273874182099	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	403	0.10771714493435403	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	383	0.10237138091776077	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.672882008296626E-4	0.0	0.0
2	0.0	0.0	2.672882008296626E-4	0.0	0.0
3	0.0	0.0	2.672882008296626E-4	0.0	0.0
4	0.0	0.0	5.345764016593252E-4	0.0	0.0
5	0.0	0.0	5.345764016593252E-4	0.0	0.0
6	0.0	0.0	0.0010691528033186504	0.0	0.0
7	0.0	0.0	0.001871017405807638	0.0	0.0
8	0.0	0.0	0.002138305606637301	0.0	0.0
9	0.0	0.0	0.0024055938074669632	2.672882008296626E-4	0.0
10	0.0	0.0	0.003207458409955951	5.345764016593252E-4	0.0
11	0.0	0.0	0.005613052217422914	8.018646024889877E-4	0.0
12	0.0	0.0	0.00721678142240089	8.018646024889877E-4	0.0
13	0.0	0.0	0.00721678142240089	8.018646024889877E-4	0.0
14	0.0	0.0	0.00721678142240089	8.018646024889877E-4	0.0
15	0.0	0.0	0.008018646024889877	8.018646024889877E-4	0.0
16	0.0	0.0	0.008553222426549203	8.018646024889877E-4	0.0
17	0.0	0.0	0.008820510627378864	8.018646024889877E-4	0.0
18	0.0	0.0	0.008820510627378864	8.018646024889877E-4	0.0
19	0.0	0.0	0.00935508702903819	8.018646024889877E-4	0.0
20	0.0	0.0	0.010156951631527179	8.018646024889877E-4	0.0
21	0.0	0.0	0.011493392635675491	0.0013364410041483128	0.0
22	0.0	0.0	0.011760680836505154	0.001871017405807638	0.0
23	0.0	0.0	0.011760680836505154	0.002138305606637301	0.0
24	0.0	0.0	0.011760680836505154	0.0024055938074669632	0.0
25	0.0	0.0	0.011760680836505154	0.0024055938074669632	0.0
26	0.0	0.0	0.011760680836505154	0.003207458409955951	0.0
27	0.0	0.0	0.011760680836505154	0.004009323012444939	0.0
28	0.0	0.0	0.011760680836505154	0.0077513578240602145	0.0
29	0.0	0.0	0.011760680836505154	0.01790830945558739	0.0
30	0.0	0.0	0.011760680836505154	0.030470854894581532	0.0
31	0.0	0.0	0.011760680836505154	0.07724629003977249	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	180	0.0	26.72222	1
GTGTGAT	40	0.0019287661	23.125	11
GTATCAA	545	0.0	23.08257	1
TCGCCAT	140	0.0	21.142857	13
CTAACCG	70	5.0867584E-6	21.142857	6
TAACCGT	80	6.934315E-7	20.8125	7
GTGTTAC	45	0.003821059	20.555553	1
GACAGTC	45	0.003821059	20.555553	7
GCTCTAG	55	5.134187E-4	20.181818	1
ACAGATC	55	5.134187E-4	20.181818	8
AGGGGTT	55	5.134187E-4	20.181818	5
CGTGCAA	75	9.238403E-6	19.733334	11
GTCGCCA	155	1.8189894E-12	19.096775	12
AACCGTG	80	1.6119253E-5	18.5	8
GTACCCT	80	1.6119253E-5	18.5	1
CTATAGG	60	9.22095E-4	18.5	4
CCTCGGC	110	3.8309736E-8	18.5	21
TAGATTG	60	9.22095E-4	18.5	15
GTATAGG	110	3.8309736E-8	18.5	1
TCTATAG	70	1.21640995E-4	18.5	3
>>END_MODULE