FastQCFastQC Report
Fri 10 Feb 2017
ERR1632978.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632978.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393119
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT85892.1848346175076756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT69641.7714737776601996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35540.9040519537341111No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19400.4934892488025254No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG18330.4662710273479532No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA13760.35002124038777066No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG13130.3339955585967608No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG12630.3212767635245307No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11970.3044879540291871No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT11550.29380416616851385No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10440.26556844110816313No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10090.2566652845576022No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9420.2396220991608139No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9230.2347889570333665No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC7830.19917633083112238No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC7050.17933501051844353No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC6810.1732299888837731No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT6760.1719581093765501No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6750.1717037334751055No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA6740.17144935757366092No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6600.16788809495343648No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6350.16152869741732148No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6330.16101994561443228No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6250.15898493840287548No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6200.15771305889565246No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6030.15338866857109423No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5950.15135366135953743No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5620.14295925661186562No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5610.14270488071042103No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5340.1358367313714168No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT5310.135073603667083No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT5250.13354734825841538No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5250.13354734825841538No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5240.1332929723569708No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5220.13278422055408157No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA5210.13252984465263698No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5190.1320210928497478No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5140.1307492133425248No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5120.1302404615396356No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5120.1302404615396356No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT5080.12922295793385716No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5020.12769670252518958No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4840.12311793629918677No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG4730.12031980138329616No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA4680.11904792187607315No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4670.11879354597462854No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4590.11675853876307175No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4580.11650416286162714No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4410.11217977253706891No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4380.11141664483273514No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4380.11141664483273514No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4380.11141664483273514No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4370.11116226893129053No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG4360.11090789302984592No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4350.11065351712840132No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG4310.10963601352262294No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC4240.10785538221251073No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG4220.10734663040962152No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4220.10734663040962152No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA4120.10480287139517551No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4120.10480287139517551No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4070.1035309918879525No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA4040.1027678641836187No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT3980.10124160877495109No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG3940.1002241051691727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGAAA801.8189894E-1230.06250212
CTAGTGG801.8189894E-1230.0625029
CTGTACT250.005491998529.64
TACTTGG250.005491998529.67
AGTCGTA250.005491998529.67
GGTATCA11050.026.9547521
ATTAGCG358.8589755E-426.4285723
TTTACCG856.91216E-1126.11764730
CGCCGTT1000.025.90000225
CCGCCGT1000.025.90000224
TTACCGG901.4188117E-1024.66666831
TACCGGG901.4188117E-1024.66666832
CGTTATT601.3336703E-624.66666610
TTGTTAC601.3336703E-624.66666615
CCGTTTA1051.8189894E-1224.66666627
GCCGTTT1051.8189894E-1224.66666626
ACTAGTG1400.023.7857138
GGACCGT1250.023.686
ATCTCGT551.8985325E-523.54545437
AGGACCG1103.6379788E-1223.5454545
Produced by FastQC (version 0.11.5)