FastQCFastQC Report
Fri 10 Feb 2017
ERR1632977.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632977.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278389
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT100253.60107619194724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT81262.9189371706497025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43021.5453196785792542No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG22900.8225899730233595No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21590.7755335160512807No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG16560.594851089662307No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11870.42638178951036143No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10860.390101620394484No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10590.3804029613239029No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10070.36172406237315413No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9540.34268595382719863No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8510.3056873655209078No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7850.28197953223726513No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA7520.2701256155954438No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6900.24785462069262792No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG6800.24426252474056084No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6790.24390331514535418No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC6630.23815596162204683No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC6510.23384544647956634No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6030.2166033859096444No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5800.20834156521989017No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5570.2000797445301359No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG5460.19612843898286209No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5430.195050810197242No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG5150.18499294153145418No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5150.18499294153145418No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5140.1846337319362475No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5050.18140084557938713No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5000.17960479760335357No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4920.17673112084169992No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4830.17349823448483956No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4800.17242060569921944No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4790.17206139610401272No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4780.17170218650880603No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4480.16092589865260482No Hit
GTTTCATACTAGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4440.15948906027177798No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4430.1591298506765713No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4420.15877064108136457No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4380.15733380270053773No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4350.1562561739149176No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4340.15589696431971092No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4250.15266407796285056No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4230.15194565877243713No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4210.15122723958202372No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4190.15050882039161032No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4170.14979040120119688No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4140.14871277241557676No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4140.14871277241557676No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4140.14871277241557676No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG4120.14799435322516336No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4090.14691672443954323No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4070.14619830524912983No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA4070.14619830524912983No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3990.14332462848747615No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC3910.1404509517258225No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3810.13685885577375542No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC3780.1357812269881353No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3760.1350628077977219No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT3710.13326675982168837No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA3660.13147071184565481No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG3640.1307522926552414No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3570.12823782548879445No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3520.12644177751276092No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA3520.12644177751276092No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3470.1246457295367274No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA3460.12428651994152067No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG3440.12356810075110727No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3420.12284968156069384No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3390.12177205277507372No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3360.1206944239894536No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3340.11997600479904018No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.11853916641821335No Hit
CTCTCTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTC3250.11674311844217981No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3230.11602469925176642No Hit
TCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCGGA3160.11351023208531948No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3150.11315102249011275No Hit
GTATCAACGCAGAGTACGGGTCAGAGTTCTACAGTCCGACGAT3140.11279181289490606Illumina DpnII expression Sequencing Primer (96% over 26bp)
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3140.11279181289490606No Hit
GTTTCATACTAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA3110.11171418410928594No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3090.1109957649188725No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT3090.1109957649188725No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3090.1109957649188725No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3060.10991813613325238No Hit
GGTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCC3050.10955892653804569No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2980.10704445937159875No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC2980.10704445937159875No Hit
CTCTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGC2960.10632604018118533No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2950.10596683058597862No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA2900.10417078260994508No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA2880.10345236341953165No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2880.10345236341953165No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC2880.10345236341953165No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2810.10093789625308472No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCCTG303.5925355E-430.83333236
GGTATCA12200.030.0245911
GAATCAA502.708275E-729.61
TCCCTGT405.9242135E-527.75000237
CTTCCCT358.8524504E-426.4285735
GACATCT509.058809E-625.90000218
GTGACAT509.058809E-625.90000216
ATTATAG601.3313311E-624.6666663
GTATCAA34400.023.8779071
TATTATA551.8959512E-523.5454542
TGGAGAC400.001927531823.1250023
CTAGTGG802.70702E-823.1250029
GTCAAAA400.001927531823.12500234
GTCTTCT400.001927531823.1250021
TTGGTGA652.6707348E-622.7692313
ACTAGTG1500.022.28
GGACCGT502.6949795E-422.26
TGACATC603.713243E-521.58333217
AGATCTT705.0790386E-621.1428578
ATAGATC705.0790386E-621.1428576
Produced by FastQC (version 0.11.5)