FastQCFastQC Report
Fri 10 Feb 2017
ERR1632976.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632976.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461704
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20050.4342609117529846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14470.31340425900576996No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG8650.18734947065652452No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7920.17153847486701435No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA7430.16092561467953495No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG6640.1438150849895171No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG6350.1375340044703966No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC5730.12410548749848388No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5260.11392580527784034No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA5160.11175991544366089No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT5150.11154332646024293No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC5120.1108935595099891No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT5060.10959402560948141No Hit
GATACGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG4780.10352953407377888No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4770.10331294509036092No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC4650.10071387728934555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4400.024.3863641
AACGATT851.9681465E-923.94117722
TACGTGA851.9681465E-923.94117736
GTACGTA400.001929447223.12513
CCTACGT903.812602E-922.6111134
ACGATTA903.812602E-922.6111123
CGATTAA855.163747E-821.76470624
CAACGAT957.1177055E-921.42105321
TTCAACG1001.2847522E-820.3519
CGCGCGA555.13669E-420.18181832
ATAAGAC656.890702E-519.9230773
GTATCAA9500.019.8631571
GTCCTAC1052.2506356E-819.3809532
TTCGTTT1052.2506356E-819.3809511
CTACGTG1052.2506356E-819.3809535
ACGTGAT1052.2506356E-819.3809537
TAGGGGT1052.2506356E-819.380954
TCAACGA1052.2506356E-819.3809520
TCGTTTG1205.149559E-918.512
TCCTACG1103.8367943E-818.533