##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632976.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	461704
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62916067437146	33.0	33.0	33.0	27.0	33.0
2	31.152898826954065	33.0	33.0	33.0	27.0	33.0
3	31.604881049330306	33.0	33.0	33.0	27.0	33.0
4	31.744697901685928	33.0	33.0	33.0	27.0	33.0
5	31.864540917990748	33.0	33.0	33.0	33.0	33.0
6	34.72487351203368	37.0	37.0	37.0	27.0	37.0
7	34.89305918943739	37.0	37.0	37.0	27.0	37.0
8	35.06767539375877	37.0	37.0	37.0	33.0	37.0
9	35.17431514563443	37.0	37.0	37.0	33.0	37.0
10	35.189838078075994	37.0	37.0	37.0	33.0	37.0
11	35.22225278533433	37.0	37.0	37.0	33.0	37.0
12	35.14907819728657	37.0	37.0	37.0	33.0	37.0
13	35.19908426177811	37.0	37.0	37.0	33.0	37.0
14	35.154038085006846	37.0	37.0	37.0	33.0	37.0
15	35.19328617469201	37.0	37.0	37.0	33.0	37.0
16	35.18969946112661	37.0	37.0	37.0	33.0	37.0
17	35.13966090828756	37.0	37.0	37.0	33.0	37.0
18	35.159318524422574	37.0	37.0	37.0	33.0	37.0
19	35.17228137508014	37.0	37.0	37.0	33.0	37.0
20	35.17368920347236	37.0	37.0	37.0	33.0	37.0
21	35.18938757299049	37.0	37.0	37.0	33.0	37.0
22	35.189734115363954	37.0	37.0	37.0	33.0	37.0
23	35.16238759031761	37.0	37.0	37.0	33.0	37.0
24	35.16063105366209	37.0	37.0	37.0	33.0	37.0
25	35.15437812971081	37.0	37.0	37.0	33.0	37.0
26	35.11880772096408	37.0	37.0	37.0	33.0	37.0
27	35.057331103910734	37.0	37.0	37.0	33.0	37.0
28	35.04250775388561	37.0	37.0	37.0	27.0	37.0
29	35.071697451180846	37.0	37.0	37.0	33.0	37.0
30	35.02572860534022	37.0	37.0	37.0	27.0	37.0
31	35.018498865073724	37.0	37.0	37.0	27.0	37.0
32	35.05333503716667	37.0	37.0	37.0	27.0	37.0
33	35.04440723927018	37.0	37.0	37.0	27.0	37.0
34	34.953286088056416	37.0	37.0	37.0	27.0	37.0
35	34.961161263493494	37.0	37.0	37.0	27.0	37.0
36	34.932287786114046	37.0	37.0	37.0	27.0	37.0
37	34.95701575035088	37.0	37.0	37.0	27.0	37.0
38	34.82550508550933	37.0	37.0	37.0	27.0	37.0
39	34.88231637585986	37.0	37.0	37.0	27.0	37.0
40	34.921096633340845	37.0	37.0	37.0	27.0	37.0
41	34.915361357059936	37.0	37.0	37.0	27.0	37.0
42	34.839349453329405	37.0	37.0	37.0	27.0	37.0
43	34.623122173513764	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	16.0
16	21.0
17	25.0
18	15.0
19	22.0
20	50.0
21	96.0
22	316.0
23	665.0
24	1449.0
25	2483.0
26	4181.0
27	6101.0
28	8536.0
29	11416.0
30	14838.0
31	19003.0
32	23834.0
33	30748.0
34	43687.0
35	78111.0
36	216090.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.176805918943735	20.32774244970804	12.934477500736403	24.56097413061182
2	16.48956907455859	23.369301543846273	37.35596832602707	22.78516105556807
3	17.93638348379048	26.554242545007188	29.977214838944434	25.5321591322579
4	12.498050699149237	17.854296259075078	39.426125829535806	30.22152721223988
5	14.299421274236307	36.511271290697074	35.22603226309497	13.963275171971654
6	29.976565071994177	39.88875989811655	17.035589901755237	13.09908512813404
7	27.57979138149117	31.88493060488971	23.10484639509296	17.430431618526153
8	24.615554554433146	35.72548645885676	20.676234124027516	18.982724862682584
9	26.048507268726283	14.862336042139553	20.261249631798727	38.82790705733544
10	15.088021762861054	27.948642420251936	34.21369535459948	22.749640462287527
11	34.473168956734185	23.805944934416857	23.566180929773186	18.154705179075773
12	23.012362899173496	25.581108242510354	30.13879022057422	21.267738637741928
13	28.02098314071353	22.141025418883096	26.21658031985861	23.621411120544764
14	22.157486181622858	20.95108554398489	27.602316635766638	29.289111638625613
15	23.74096823939147	28.82842687089564	24.840157330237556	22.590447559475336
16	23.712378493580303	28.256631954672258	24.84903747855769	23.18195207318975
17	23.000017327118673	27.274400914871865	25.821305425120855	23.904276332888603
18	22.844723026008005	26.737260235995358	28.008204390691873	22.409812347304765
19	25.240630360577338	26.142073709562837	27.199027948642417	21.418267981217404
20	24.573319702666645	26.23109178174761	27.33916102091383	21.856427494671912
21	22.844289848041168	27.278732694540224	27.141198690069828	22.735778767348776
22	23.51961429833833	26.142073709562837	27.341976677698266	22.996335314400568
23	22.459411224507477	26.719283350371665	28.10436989932944	22.716935525791413
24	23.019077157659453	27.51892987715073	26.290437163204132	23.17155580198569
25	23.278334170810737	27.19253027913988	27.53495746192366	21.994178088125725
26	23.903409976954933	26.85508464297472	26.655389600263373	22.586115779806974
27	23.415001819347463	26.681596867256946	26.274192989447787	23.62920832394781
28	22.83151109801951	26.45547796856861	27.81197477171521	22.901036161696673
29	24.163533346039888	26.973775405887757	26.933706443955437	21.928984804116926
30	23.875686587077436	26.493381040666748	26.71646769358723	22.914464678668583
31	22.995685547450314	26.288921040320208	27.395040978635667	23.32035243359382
32	22.35891393620155	26.49619669745118	27.460884029594716	23.684005336752552
33	22.817216225113928	26.09745637897874	27.87196992012198	23.21335747578535
34	23.518098175454405	26.28567220556894	27.83146778022283	22.364761838753832
35	23.345693344653718	27.04416682549859	26.479735934711417	23.13040389513628
36	23.3599882175593	26.62290125275068	26.465441061805834	23.551669467884185
37	23.143399234141356	26.387035849808537	27.45243705924142	23.017127856808692
38	23.28743090811429	26.06713392130023	26.70000693084747	23.945428239738014
39	22.66798641553896	26.524353265295513	27.730320724966646	23.077339594198882
40	23.213790653752188	26.939987524474557	27.403271360005544	22.442950461767712
41	23.004565695770452	26.165681908755396	27.460017673661046	23.36973472181311
42	22.30173444457921	27.758693881794393	26.999116316947653	22.940455356678736
43	23.80204633271533	25.560748878069067	26.9272087744529	23.709996014762705
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10.0
1	20.0
2	30.0
3	117.5
4	205.0
5	205.0
6	267.0
7	329.0
8	308.0
9	287.0
10	386.0
11	485.0
12	485.0
13	746.5
14	1008.0
15	1788.0
16	2568.0
17	2946.0
18	3324.0
19	3324.0
20	3054.5
21	2785.0
22	3118.0
23	3451.0
24	4530.5
25	5610.0
26	5610.0
27	7110.5
28	8611.0
29	10357.5
30	12104.0
31	14750.5
32	17397.0
33	17397.0
34	21375.0
35	25353.0
36	27611.0
37	29869.0
38	31293.0
39	32717.0
40	32717.0
41	33441.5
42	34166.0
43	35175.0
44	36184.0
45	35524.5
46	34865.0
47	34865.0
48	33380.0
49	31895.0
50	32551.0
51	33207.0
52	33233.5
53	33260.0
54	33260.0
55	31923.0
56	30586.0
57	25756.0
58	20926.0
59	19316.0
60	17706.0
61	17706.0
62	16081.0
63	14456.0
64	11341.0
65	8226.0
66	7108.0
67	5990.0
68	5990.0
69	5217.0
70	4444.0
71	4371.0
72	4298.0
73	3861.0
74	3424.0
75	3424.0
76	2139.5
77	855.0
78	650.5
79	446.0
80	360.0
81	274.0
82	274.0
83	208.0
84	142.0
85	135.5
86	129.0
87	94.0
88	59.0
89	59.0
90	37.5
91	16.0
92	11.5
93	7.0
94	3.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	461704.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.861550829821006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.40485859803198	53.5191348739905
2	5.8719192976463965	7.030043910093332
3	1.5449792995229927	2.7745457060825065
4	0.7274715238093258	1.741902943990372
5	0.42787111705062814	1.2806514310969235
6	0.2933523230077085	1.0536314996863207
7	0.21198677804272975	0.8882900102338396
8	0.16489134822356316	0.7896521458466035
9	0.142695355637193	0.7687768756189692
>10	0.9723799274511749	11.763523542764684
>50	0.13644396913781393	5.800625437708852
>100	0.09642040484248826	10.346031424027007
>500	0.004002356427424041	1.4913218071264378
>1k	7.277011686225529E-4	0.7518683917336736
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2005	0.4342609117529846	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1447	0.31340425900576996	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	865	0.18734947065652452	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	792	0.17153847486701435	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	743	0.16092561467953495	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	664	0.1438150849895171	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	635	0.1375340044703966	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	573	0.12410548749848388	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	526	0.11392580527784034	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	516	0.11175991544366089	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	515	0.11154332646024293	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	512	0.1108935595099891	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	506	0.10959402560948141	No Hit
GATACGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	478	0.10352953407377888	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	477	0.10331294509036092	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	465	0.10071387728934555	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.1658898341794742E-4	0.0	0.0
2	0.0	0.0	2.1658898341794742E-4	0.0	0.0
3	0.0	0.0	6.497669502538423E-4	0.0	0.0
4	0.0	0.0	8.663559336717897E-4	0.0	0.0
5	0.0	0.0	0.0010829449170897372	0.0	0.0
6	2.1658898341794742E-4	0.0	0.0023824788175974216	0.0	0.0
7	2.1658898341794742E-4	0.0	0.006930847469374318	0.0	0.0
8	2.1658898341794742E-4	0.0	0.008013792386464056	0.0	0.0
9	2.1658898341794742E-4	0.0	0.011479216121151214	2.1658898341794742E-4	0.0
10	0.0010829449170897372	0.0	0.01256216103824095	4.3317796683589485E-4	0.0
11	0.0010829449170897372	0.0	0.017327118673435796	4.3317796683589485E-4	0.0
12	0.0010829449170897372	0.0	0.01862665257394348	6.497669502538423E-4	0.0
13	0.0010829449170897372	0.0	0.019059830540779372	6.497669502538423E-4	0.0
14	0.0010829449170897372	0.0	0.02187548732521269	6.497669502538423E-4	0.0
15	0.0010829449170897372	0.0	0.024257966142810113	8.663559336717897E-4	0.0
16	0.0012995339005076845	0.0	0.026423855976989586	8.663559336717897E-4	0.0
17	0.0012995339005076845	0.0	0.027290211910661374	0.0010829449170897372	0.0
18	0.0012995339005076845	0.0	0.02793997886091522	0.0010829449170897372	0.0
19	0.0012995339005076845	0.0	0.028373156827751114	0.0010829449170897372	0.0
20	0.0012995339005076845	0.0	0.02945610174484085	0.0012995339005076845	0.0
21	0.0012995339005076845	0.0	0.03032245767851264	0.0017327118673435794	0.0
22	0.0012995339005076845	0.0	0.03118881361218443	0.0021658898341794745	0.0
23	0.0012995339005076845	0.0	0.03118881361218443	0.0032488347512692113	0.0
24	0.0012995339005076845	0.0	0.03118881361218443	0.003465423734687159	0.0
25	0.0012995339005076845	0.0	0.03118881361218443	0.003465423734687159	0.0
26	0.0012995339005076845	0.0	0.03118881361218443	0.0036820127181051063	0.0
27	0.0012995339005076845	0.0	0.03118881361218443	0.004981546618612791	0.0
28	0.0012995339005076845	0.0	0.03118881361218443	0.013861694938748635	0.0
29	0.0012995339005076845	0.0	0.03118881361218443	0.036820127181051064	0.0
30	0.0012995339005076845	0.0	0.03118881361218443	0.06562646197563807	0.0
31	0.0012995339005076845	0.0	0.03140540259560238	0.14554779685686067	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	440	0.0	24.386364	1
AACGATT	85	1.9681465E-9	23.941177	22
TACGTGA	85	1.9681465E-9	23.941177	36
GTACGTA	40	0.0019294472	23.125	13
CCTACGT	90	3.812602E-9	22.61111	34
ACGATTA	90	3.812602E-9	22.61111	23
CGATTAA	85	5.163747E-8	21.764706	24
CAACGAT	95	7.1177055E-9	21.421053	21
TTCAACG	100	1.2847522E-8	20.35	19
CGCGCGA	55	5.13669E-4	20.181818	32
ATAAGAC	65	6.890702E-5	19.923077	3
GTATCAA	950	0.0	19.863157	1
GTCCTAC	105	2.2506356E-8	19.38095	32
TTCGTTT	105	2.2506356E-8	19.38095	11
CTACGTG	105	2.2506356E-8	19.38095	35
ACGTGAT	105	2.2506356E-8	19.38095	37
TAGGGGT	105	2.2506356E-8	19.38095	4
TCAACGA	105	2.2506356E-8	19.38095	20
TCGTTTG	120	5.149559E-9	18.5	12
TCCTACG	110	3.8367943E-8	18.5	33
>>END_MODULE