FastQCFastQC Report
Fri 10 Feb 2017
ERR1632975.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632975.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466176
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23740.5092497254255903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18290.3923410900604064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8650.18555223778143878No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA7090.15208848160351457No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC6720.1441515650741351No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6480.139003294892916No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA6040.12956479956068095No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT5810.12463104063701264No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT5280.11326194398682042No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA5080.10897171883580452No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5080.10897171883580452No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG5040.10811367380560132No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA4980.10682660626029654No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC4810.10317991488193301No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCC200.001840814137.012
CGACAAA250.00549303429.59999817
TATACGG250.00549303429.5999982
GGTATCA3650.027.3698641
GTATCAA10850.024.7235031
TAGTACC451.3215642E-424.6666684
CGAGTCG601.3345598E-624.66666621
CGTGGGA1850.024.037
TCTATAC603.720173E-521.5833323
GAGTCGC705.091193E-621.14285922
ACGTGGG2100.021.14285936
GGGTAGG450.003822447720.55555711
TTGACCG450.003822447720.5555574
GTATTAT1303.274181E-1119.9230751
ATATACT759.246407E-619.7333344
TATTCCG851.2420605E-619.5882365
GCCGCTC1254.110916E-1019.2427
TTCGCCG1254.110916E-1019.2424
GCCACGT2250.018.91111233
CGCCGTC701.2172251E-418.526
Produced by FastQC (version 0.11.5)