FastQCFastQC Report
Fri 10 Feb 2017
ERR1632974.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632974.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368832
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT76852.0836044594829084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57351.5549084678119034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31730.8602832726010758No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14440.3915061599861183No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG13970.37876323095609926No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG9620.26082335589102895No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8510.23072835328821795No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8180.22178119035224708No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7890.21391853201457575No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7790.211207270518827No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7060.19141506159986119No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG6420.17406298802706924No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6200.168098212736422No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC6120.16592920353982302No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5810.1575242929030019No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG5740.15562640985597778No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5700.1545419052576783No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5680.15399965295852855No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA5640.15291514836022904No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5560.15074613916363006No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG5490.14884825611660593No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC5480.14857712996703107No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG5360.14532361617213257No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT5210.14125672392850946No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5140.13935884088148534No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5080.13773208398403608No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5040.1366475793857366No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC4910.13312293944126322No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4890.1325806871421135No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4860.13176730869338885No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC4840.13122505639423912No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4770.129327173347215No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4720.12797154259934063No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG4680.12688703800104112No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG4590.12444690265486726No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT4530.12282014575741801No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA4480.12146451500954364No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4330.11739762276592053No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT4320.11712649661634565No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4250.11522861356932153No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA4240.11495748741974665No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4220.11441523512059691No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4190.11360185667187228No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4140.11224622592399791No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT4120.11170397362484817No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC4100.11116172132569842No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4060.11007721672739892No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC4050.10980609057782405No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4030.1092638382786743No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG4000.10845045982994968No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT3990.1081793336803748No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG3980.10790820753079992No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG3960.10736595523165018No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3860.10465469373590144No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3860.10465469373590144No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT3830.10384131528717681No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3790.10275681068887732No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3770.10221455838972757No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA3710.10058780149227832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGGG303.5947806E-430.8333343
TATCTTG303.5947806E-430.83333423
AGATAAG250.00549156329.613
GGACGGT250.00549156329.619
CCCTTAG250.00549156329.630
TGAGGGC250.00549156329.631
GCCAGTG250.00549156329.637
TTGTTAC502.7123133E-729.615
AGGGCGT250.00549156329.633
GACTAGG250.00549156329.62
ATTGTTA453.998559E-628.77777714
CGTTATT453.998559E-628.77777710
TTATTGT453.998559E-628.77777712
ACTAGTG754.6020432E-1027.1333338
TGTTACG556.2423896E-726.9090916
GGTATCA10950.026.6940651
TAATAGA358.8579295E-426.4285724
GATAAGG358.8579295E-426.42857214
GGCCGCC706.561095E-926.42857222
AGATTCT358.8579295E-426.42857217
Produced by FastQC (version 0.11.5)