##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632974.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	368832
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.837421915668923	33.0	33.0	33.0	33.0	33.0
2	31.319110055526636	33.0	33.0	33.0	27.0	33.0
3	31.745857192434496	33.0	33.0	33.0	27.0	33.0
4	31.84402925993406	33.0	33.0	33.0	27.0	33.0
5	31.971190135346173	33.0	33.0	33.0	33.0	33.0
6	34.77826490109318	37.0	37.0	37.0	27.0	37.0
7	34.93607116519174	37.0	37.0	37.0	27.0	37.0
8	35.167686100989066	37.0	37.0	37.0	33.0	37.0
9	35.195720544855114	37.0	37.0	37.0	33.0	37.0
10	35.189289432587195	37.0	37.0	37.0	33.0	37.0
11	35.27510628145063	37.0	37.0	37.0	33.0	37.0
12	35.161485445948294	37.0	37.0	37.0	33.0	37.0
13	35.22562304789172	37.0	37.0	37.0	33.0	37.0
14	35.14490337064029	37.0	37.0	37.0	33.0	37.0
15	35.23708897275724	37.0	37.0	37.0	33.0	37.0
16	35.22317477876106	37.0	37.0	37.0	33.0	37.0
17	35.23261810255075	37.0	37.0	37.0	33.0	37.0
18	35.211700720111054	37.0	37.0	37.0	33.0	37.0
19	35.23084493753254	37.0	37.0	37.0	33.0	37.0
20	35.13535973017525	37.0	37.0	37.0	33.0	37.0
21	35.21735912285268	37.0	37.0	37.0	33.0	37.0
22	35.21854665538782	37.0	37.0	37.0	33.0	37.0
23	35.217909508936316	37.0	37.0	37.0	33.0	37.0
24	35.23248525073746	37.0	37.0	37.0	33.0	37.0
25	35.18649141072358	37.0	37.0	37.0	33.0	37.0
26	35.17763914193996	37.0	37.0	37.0	33.0	37.0
27	35.07347518653479	37.0	37.0	37.0	33.0	37.0
28	35.077395670657644	37.0	37.0	37.0	33.0	37.0
29	35.12575373069582	37.0	37.0	37.0	33.0	37.0
30	35.05947965469374	37.0	37.0	37.0	27.0	37.0
31	35.03278457400659	37.0	37.0	37.0	27.0	37.0
32	35.04490120163109	37.0	37.0	37.0	27.0	37.0
33	34.98754175342703	37.0	37.0	37.0	27.0	37.0
34	34.91501008589277	37.0	37.0	37.0	27.0	37.0
35	34.85099177945514	37.0	37.0	37.0	27.0	37.0
36	34.79939918445254	37.0	37.0	37.0	27.0	37.0
37	34.78794139337151	37.0	37.0	37.0	27.0	37.0
38	34.5702840317543	37.0	37.0	37.0	27.0	37.0
39	34.592866128752384	37.0	37.0	37.0	27.0	37.0
40	34.57115163543293	37.0	37.0	37.0	27.0	37.0
41	34.52589796980739	37.0	37.0	37.0	27.0	37.0
42	34.31136940395627	37.0	37.0	37.0	27.0	37.0
43	34.070679875932676	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	19.0
16	24.0
17	25.0
18	27.0
19	28.0
20	38.0
21	100.0
22	219.0
23	523.0
24	1028.0
25	1877.0
26	3004.0
27	4805.0
28	6883.0
29	9364.0
30	12210.0
31	15831.0
32	19722.0
33	25959.0
34	35487.0
35	63320.0
36	168339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.5528045288912	18.940601683151137	14.51961868818324	21.986975099774423
2	16.256995054659033	23.792675255943085	38.87108493840014	21.079244750997745
3	17.570601249349295	29.77426036786396	30.68741323963214	21.967725143154606
4	11.220284574006593	18.43115564809995	40.96417881311817	29.384380964775293
5	13.699733211868818	36.487886083636994	33.53450893631789	16.277871768176297
6	25.3831012493493	43.221304008329	16.059072965469372	15.336521776852333
7	24.94441913933715	34.33758459135866	22.236140031233735	18.48185623807045
8	24.72236682283533	33.682814940135344	19.312044508068716	22.28277372896061
9	28.35789736248482	13.236920874544507	19.48095609925386	38.92422566371682
10	17.220577390248135	26.481162155127535	31.145887558563246	25.15237289606108
11	34.29311990282839	24.787165972583725	21.885845045982997	19.033869078604894
12	24.90537697379837	27.67330383480826	26.994403956272773	20.426915235120596
13	29.71135910116259	23.272926427208052	25.360055526635435	21.655658944993927
14	23.62267916015964	21.567542946382094	29.620531841055005	25.189246052403263
15	26.27998655214298	26.387623633524203	27.570004771820233	19.76238504251258
16	20.603147232344266	28.739914107235816	26.27049713690786	24.386441523512058
17	21.820503643935453	25.451153912892593	25.67808650008676	27.0502559430852
18	24.342790213430504	23.411743015790385	29.688313378448722	22.557153392330385
19	27.055678466076692	23.24500043380184	30.381582942911677	19.31773815720979
20	25.726618080860664	22.72552273121638	30.577607149054316	20.970252038868644
21	22.137178986638904	25.96439571403783	30.665994273815723	21.232431025507548
22	22.472290907513447	25.377949852507374	30.46183628318584	21.687922956793336
23	21.636137862224537	25.62196338712476	31.671872288738506	21.070026461912196
24	22.28277372896061	25.961142200242932	29.674485944820404	22.081598125976054
25	21.95769347562034	25.681068887732085	32.18619859448204	20.175039042165537
26	21.96908077390248	26.054138469547112	30.768208832205445	21.20857192434496
27	22.616258892937708	25.348939354502864	30.777427121290994	21.257374631268437
28	21.024206142634043	25.322368991844524	31.463105153565852	22.190319711955578
29	23.298141159118515	25.454678552837063	29.532144716293594	21.715035571750825
30	23.143599253860835	24.846813725490197	31.143989675516227	20.865597345132745
31	21.761940395627278	25.522188964081206	30.926817629706747	21.789053010584762
32	19.996095783446123	25.13908771473191	31.370922262710394	23.493894239111572
33	20.147926427208052	25.32399574874197	32.30440959569669	22.22366822835329
34	21.43279325004338	25.292273989241714	31.556914801318758	21.71801795939615
35	21.113948898143327	26.723006680548327	30.632645757418008	21.530398663890335
36	22.227192868297763	26.25178943258719	30.320579559257332	21.200438139857713
37	21.767091792469202	26.072846173867774	30.39269911504425	21.767362918618772
38	21.680602550754816	26.21139163630054	29.560341835849385	22.547663977095265
39	20.930125368731563	25.770811643241366	30.294822575047718	23.004240412979353
40	20.819505899705014	27.331413760194344	31.02794768349818	20.821132656602465
41	21.638035745271562	25.546590317542943	30.02478093007114	22.790593007114353
42	20.281049366649313	27.594406125281974	30.042675255943085	22.081869252125628
43	21.620954797848345	26.073388426166925	29.798119469026545	22.50753730695818
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	43.0
1	61.0
2	79.0
3	394.5
4	710.0
5	710.0
6	923.0
7	1136.0
8	1122.0
9	1108.0
10	1484.5
11	1861.0
12	1861.0
13	2932.0
14	4003.0
15	6864.5
16	9726.0
17	10232.5
18	10739.0
19	10739.0
20	8243.5
21	5748.0
22	4005.0
23	2262.0
24	2205.0
25	2148.0
26	2148.0
27	2539.0
28	2930.0
29	3645.5
30	4361.0
31	6293.5
32	8226.0
33	8226.0
34	11007.5
35	13789.0
36	15270.5
37	16752.0
38	18806.5
39	20861.0
40	20861.0
41	23133.5
42	25406.0
43	27316.0
44	29226.0
45	30688.5
46	32151.0
47	32151.0
48	32667.5
49	33184.0
50	34928.5
51	36673.0
52	34135.0
53	31597.0
54	31597.0
55	28191.0
56	24785.0
57	20411.0
58	16037.0
59	14739.5
60	13442.0
61	13442.0
62	10669.0
63	7896.0
64	5840.5
65	3785.0
66	3655.0
67	3525.0
68	3525.0
69	2796.0
70	2067.0
71	1642.0
72	1217.0
73	1168.0
74	1119.0
75	1119.0
76	641.5
77	164.0
78	106.0
79	48.0
80	33.0
81	18.0
82	18.0
83	13.5
84	9.0
85	5.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	368832.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.691642807565508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.22240631496744	19.32587194169703
2	9.031332116210598	4.640595176123546
3	3.840268470541057	2.9598841749089018
4	2.257305374687365	2.3197553357626237
5	1.4763769140662102	1.896527416276245
6	1.0911892274084785	1.6820666319625195
7	0.8041452526936755	1.446186881832379
8	0.6215768423052164	1.2775464167968071
9	0.543483996243101	1.256669703279542
>10	4.067159847613419	21.480240326218983
>50	0.56881140577676	10.249110706229395
>100	0.44745090176131025	22.435417751171265
>500	0.023216792072520816	3.761061946902655
>1k	0.0031659261917073842	1.6305526635432932
>5k	0.002110617461138256	3.6385129272948116
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7685	2.0836044594829084	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5735	1.5549084678119034	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3173	0.8602832726010758	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1444	0.3915061599861183	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1397	0.37876323095609926	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	962	0.26082335589102895	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	851	0.23072835328821795	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	818	0.22178119035224708	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	789	0.21391853201457575	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	779	0.211207270518827	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	706	0.19141506159986119	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	642	0.17406298802706924	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	620	0.168098212736422	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	612	0.16592920353982302	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	581	0.1575242929030019	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	574	0.15562640985597778	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	570	0.1545419052576783	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	568	0.15399965295852855	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	564	0.15291514836022904	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	556	0.15074613916363006	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	549	0.14884825611660593	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	548	0.14857712996703107	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	536	0.14532361617213257	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	521	0.14125672392850946	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	514	0.13935884088148534	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	508	0.13773208398403608	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	504	0.1366475793857366	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	491	0.13312293944126322	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	489	0.1325806871421135	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	486	0.13176730869338885	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	484	0.13122505639423912	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	477	0.129327173347215	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	472	0.12797154259934063	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	468	0.12688703800104112	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	459	0.12444690265486726	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	453	0.12282014575741801	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	448	0.12146451500954364	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	433	0.11739762276592053	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	432	0.11712649661634565	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	425	0.11522861356932153	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	424	0.11495748741974665	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	422	0.11441523512059691	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	419	0.11360185667187228	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	414	0.11224622592399791	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT	412	0.11170397362484817	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	410	0.11116172132569842	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	406	0.11007721672739892	No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC	405	0.10980609057782405	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	403	0.1092638382786743	No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG	400	0.10845045982994968	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	399	0.1081793336803748	No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG	398	0.10790820753079992	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	396	0.10736595523165018	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	386	0.10465469373590144	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	386	0.10465469373590144	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	383	0.10384131528717681	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	379	0.10275681068887732	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	377	0.10221455838972757	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	371	0.10058780149227832	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0021690091965989935	0.0	0.0
2	0.0	0.0	0.002711261495748742	0.0	0.0
3	0.0	0.0	0.004066892243623113	0.0	0.0
4	0.0	0.0	0.005964775290647232	5.422522991497484E-4	0.0
5	5.422522991497484E-4	0.0	0.008676036786395974	5.422522991497484E-4	0.0
6	5.422522991497484E-4	0.0	0.0178943258719417	5.422522991497484E-4	0.0
7	5.422522991497484E-4	0.0	0.040940048585806	0.0010845045982994968	0.0
8	5.422522991497484E-4	0.0	0.046904823876453235	0.0010845045982994968	0.0
9	5.422522991497484E-4	0.0	0.057207617560298456	0.0016267568974492452	0.0
10	0.006507027589796981	0.0	0.06696815894499393	0.0016267568974492452	0.0
11	0.006778153739371855	0.0	0.09380964775290647	0.0018978830470241194	0.0
12	0.006778153739371855	0.0	0.10031667534270346	0.0021690091965989935	0.0
13	0.006778153739371855	0.0	0.11116172132569842	0.0021690091965989935	0.0
14	0.006778153739371855	0.0	0.11712649661634565	0.0021690091965989935	0.0
15	0.006778153739371855	0.0	0.1301405517959396	0.0024401353461738677	0.0
16	0.007049279888946729	0.0	0.14098559777893457	0.0024401353461738677	0.0
17	0.007049279888946729	0.0	0.1439679854242582	0.0024401353461738677	0.0
18	0.007320406038521603	0.0	0.14857712996703107	0.002711261495748742	0.0
19	0.007320406038521603	0.0	0.15264402221065418	0.002711261495748742	0.0
20	0.007320406038521603	0.0	0.15725316675342704	0.002982387645323616	0.0
21	0.007320406038521603	0.0	0.163760194343224	0.004066892243623113	0.0
22	0.007320406038521603	0.0	0.16592920353982302	0.005422522991497484	0.0
23	0.007320406038521603	0.0	0.16836933888599687	0.007049279888946729	0.0
24	0.007320406038521603	0.0	0.170267221933021	0.010845045982994968	0.0
25	0.007320406038521603	0.0	0.1713517265313205	0.011658424431719591	0.0
26	0.007320406038521603	0.0	0.1721651049800451	0.012742929030019088	0.0
27	0.007320406038521603	0.0	0.17270735727919487	0.014369685927468333	0.0
28	0.007320406038521603	0.0	0.17270735727919487	0.034975273295158774	0.0
29	0.007320406038521603	0.0	0.17270735727919487	0.0913695124067326	0.0
30	0.007320406038521603	0.0	0.17270735727919487	0.16321794204407428	0.0
31	0.007320406038521603	0.0	0.17270735727919487	0.3226401179941003	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGGG	30	3.5947806E-4	30.833334	3
TATCTTG	30	3.5947806E-4	30.833334	23
AGATAAG	25	0.005491563	29.6	13
GGACGGT	25	0.005491563	29.6	19
CCCTTAG	25	0.005491563	29.6	30
TGAGGGC	25	0.005491563	29.6	31
GCCAGTG	25	0.005491563	29.6	37
TTGTTAC	50	2.7123133E-7	29.6	15
AGGGCGT	25	0.005491563	29.6	33
GACTAGG	25	0.005491563	29.6	2
ATTGTTA	45	3.998559E-6	28.777777	14
CGTTATT	45	3.998559E-6	28.777777	10
TTATTGT	45	3.998559E-6	28.777777	12
ACTAGTG	75	4.6020432E-10	27.133333	8
TGTTACG	55	6.2423896E-7	26.90909	16
GGTATCA	1095	0.0	26.694065	1
TAATAGA	35	8.8579295E-4	26.428572	4
GATAAGG	35	8.8579295E-4	26.428572	14
GGCCGCC	70	6.561095E-9	26.428572	22
AGATTCT	35	8.8579295E-4	26.428572	17
>>END_MODULE