FastQCFastQC Report
Fri 10 Feb 2017
ERR1632973.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632973.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258464
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7430.2874675003095209No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5730.22169431719697907No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3370.13038566299368579No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC2670.10330258759440386No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG303.5918294E-430.8333345
GGTATCA1450.029.3448281
TAACGGC556.2302206E-726.9090936
AACGGCC556.2302206E-726.9090937
TTAACGG556.2302206E-726.9090935
CCGCCCT400.001927160823.12500215
GTATCAA4450.022.449441
TTTAACG806.917253E-720.812534
ATAGCTG450.003817908720.5555555
CTGTGAA555.1282824E-420.1818189
CCGCTCT609.2104304E-418.528
TATTAGA1205.118636E-918.52
TTTATAC609.2104304E-418.53
ACTAGAA500.007020497718.49999828
TCATATC500.007020497718.4999983
GTTTAAC953.584555E-617.52631633
CTCTAAT650.001575458217.0769231
TTATACT1107.7501863E-716.8181824
ACTGCTC1251.6430386E-716.288
TAAACAA700.002585210415.8571425
Produced by FastQC (version 0.11.5)