##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632973.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	258464
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.180102451405226	33.0	33.0	33.0	27.0	33.0
2	30.812972019314103	33.0	33.0	33.0	27.0	33.0
3	31.240675684041104	33.0	33.0	33.0	27.0	33.0
4	31.40934133960629	33.0	33.0	33.0	27.0	33.0
5	31.51593258635632	33.0	33.0	33.0	27.0	33.0
6	34.218583632536834	37.0	33.0	37.0	27.0	37.0
7	34.45277098551443	37.0	37.0	37.0	27.0	37.0
8	34.595046118608394	37.0	37.0	37.0	27.0	37.0
9	34.69785347282407	37.0	37.0	37.0	27.0	37.0
10	34.73921319796954	37.0	37.0	37.0	27.0	37.0
11	34.72280085427758	37.0	37.0	37.0	27.0	37.0
12	34.718436610127526	37.0	37.0	37.0	27.0	37.0
13	34.739952179026865	37.0	37.0	37.0	27.0	37.0
14	34.73290284140151	37.0	37.0	37.0	27.0	37.0
15	34.73036090132475	37.0	37.0	37.0	27.0	37.0
16	34.753455026618795	37.0	37.0	37.0	27.0	37.0
17	34.70708880153522	37.0	37.0	37.0	27.0	37.0
18	34.693752321406464	37.0	37.0	37.0	27.0	37.0
19	34.714350934753	37.0	37.0	37.0	27.0	37.0
20	34.72745914324626	37.0	37.0	37.0	27.0	37.0
21	34.713023864058435	37.0	37.0	37.0	27.0	37.0
22	34.69762907019933	37.0	37.0	37.0	27.0	37.0
23	34.66928082827783	37.0	37.0	37.0	27.0	37.0
24	34.666216571746936	37.0	37.0	37.0	27.0	37.0
25	34.67580011142751	37.0	37.0	37.0	27.0	37.0
26	34.61101739507243	37.0	37.0	37.0	27.0	37.0
27	34.55885926086418	37.0	37.0	37.0	27.0	37.0
28	34.50813266064133	37.0	37.0	37.0	27.0	37.0
29	34.5655487804878	37.0	37.0	37.0	27.0	37.0
30	34.51524777144979	37.0	37.0	37.0	27.0	37.0
31	34.51151804506624	37.0	37.0	37.0	27.0	37.0
32	34.536264237959635	37.0	37.0	37.0	27.0	37.0
33	34.4970324687384	37.0	37.0	37.0	27.0	37.0
34	34.45744088151542	37.0	37.0	37.0	27.0	37.0
35	34.43099232388263	37.0	37.0	37.0	27.0	37.0
36	34.39433344682432	37.0	37.0	37.0	27.0	37.0
37	34.440699671907886	37.0	37.0	37.0	27.0	37.0
38	34.287088337253934	37.0	37.0	37.0	27.0	37.0
39	34.36473938343445	37.0	37.0	37.0	27.0	37.0
40	34.39364863191779	37.0	37.0	37.0	27.0	37.0
41	34.436904172341215	37.0	37.0	37.0	27.0	37.0
42	34.321452890924846	37.0	37.0	37.0	27.0	37.0
43	34.077887055837564	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	7.0
16	10.0
17	15.0
18	13.0
19	12.0
20	27.0
21	124.0
22	317.0
23	702.0
24	1323.0
25	2260.0
26	3486.0
27	4683.0
28	6460.0
29	8315.0
30	10534.0
31	12542.0
32	15828.0
33	19773.0
34	27353.0
35	47040.0
36	97639.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.13402253311873	21.055543518633154	13.422759068961248	25.387674879286866
2	16.09701931410177	23.366116751269036	37.18080661136561	23.356057323263588
3	17.910037761545127	26.945725516899838	30.23980128760678	24.90443543394825
4	12.989042961495606	18.67068527918782	38.47034790144856	29.86992385786802
5	14.30295901943791	36.926225702612356	35.33567537452024	13.43513990342949
6	29.60605732326359	39.95024452148075	17.482512071313607	12.961186083942058
7	26.34757645165284	32.48266683174446	23.778166398415255	17.391590318187447
8	24.20801349510957	36.53158660393711	20.629178531633034	18.63122136932029
9	25.640321282654448	15.590565804135197	20.238021542651975	38.53109137055837
10	14.733966819363625	28.29330196855268	34.28988176303083	22.682849449052867
11	33.81863625108332	23.7359941810078	24.36548223350254	18.07988733440634
12	22.75365234616813	25.855438281540177	30.371734554909004	21.01917481738269
13	27.730360901324747	22.440649374767858	26.112340596756223	23.71664912715117
14	21.856041847220503	21.065602946638602	27.55045190045809	29.527903305682802
15	23.583168255540425	29.120496471462175	24.17087099170484	23.125464281292558
16	22.947489785811566	28.64151293797202	25.181069704098057	23.22992757211836
17	22.31103751392844	27.687801782840165	26.616472700259997	23.3846880029714
18	22.90686517271264	27.568636251083323	27.61777268787916	21.906725888324875
19	24.324857620403613	26.683019685526805	27.106289463909867	21.88583323015971
20	23.760368948867153	26.684954190912467	27.327983781106845	22.22669307911353
21	23.04653646155751	27.227776402129507	27.210365853658537	22.51532128265445
22	22.844961000371423	27.157360406091367	27.167032933019687	22.83064566051752
23	22.688652965209855	27.089652717593165	27.673099541909124	22.548594775287857
24	23.005911848458585	27.525303330444473	26.334808716107467	23.133976104989475
25	23.155642565308902	26.921350749040485	27.485452519499816	22.4375541661508
26	22.989662003219017	27.205336139655813	27.28851987123932	22.51648198588585
27	23.109988238207254	26.981707317073173	26.83700631422558	23.071298130493993
28	22.663117494119103	27.043224588337257	27.42703045685279	22.86662746069085
29	23.69111365606042	27.012272502166645	26.727513309397054	22.56910053237588
30	23.215225331187323	26.84590503899963	27.358935867277456	22.579933762535596
31	23.333230159712766	26.86641079608766	27.133372539309146	22.66698650489043
32	22.38687012504643	26.935279187817258	27.369769097437167	23.308081589699146
33	22.334251578556394	27.062182741116754	27.84952643308159	22.754039247245263
34	22.998560727993066	26.645877182122074	27.58759440386282	22.76796768602204
35	22.85463352729974	27.122539309149435	27.281168750773805	22.74165841277702
36	23.568466014609385	26.97280859229912	26.826559985143	22.632165407948495
37	22.843413396062896	26.742989352482354	27.15465209855144	23.25894515290331
38	23.008620155998514	27.12756902315216	26.89968428872106	22.964126532128265
39	22.602374025009286	26.960427757830878	27.39066175560233	23.04653646155751
40	22.5586542032933	27.512922495976227	27.479649003342825	22.448774297387644
41	22.572582642070074	26.468289587718214	27.569796954314718	23.38933081589699
42	22.526154512814163	27.361257273740254	27.204949238578678	22.907638974866906
43	23.037250835706327	26.375046428129256	27.493577442119598	23.09412529404482
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	7.0
2	8.0
3	31.0
4	54.0
5	54.0
6	76.0
7	98.0
8	105.0
9	112.0
10	143.5
11	175.0
12	175.0
13	281.0
14	387.0
15	730.5
16	1074.0
17	1237.5
18	1401.0
19	1401.0
20	1382.0
21	1363.0
22	1615.5
23	1868.0
24	2518.5
25	3169.0
26	3169.0
27	4237.5
28	5306.0
29	6471.5
30	7637.0
31	9074.0
32	10511.0
33	10511.0
34	12119.0
35	13727.0
36	15102.0
37	16477.0
38	17842.0
39	19207.0
40	19207.0
41	20180.5
42	21154.0
43	21542.5
44	21931.0
45	21397.5
46	20864.0
47	20864.0
48	20237.0
49	19610.0
50	19343.0
51	19076.0
52	18075.0
53	17074.0
54	17074.0
55	16273.0
56	15472.0
57	13386.5
58	11301.0
59	10387.0
60	9473.0
61	9473.0
62	8221.5
63	6970.0
64	5828.0
65	4686.0
66	3911.5
67	3137.0
68	3137.0
69	2595.0
70	2053.0
71	1667.5
72	1282.0
73	1047.5
74	813.0
75	813.0
76	611.0
77	409.0
78	321.5
79	234.0
80	194.0
81	154.0
82	154.0
83	120.0
84	86.0
85	68.0
86	50.0
87	45.0
88	40.0
89	40.0
90	25.5
91	11.0
92	7.5
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	258464.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.2295790059217
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.90581831050082	64.0395358843986
2	5.6189903016407134	8.004766272484499
3	1.5776621460233984	3.3712863142447693
4	0.7421905225773028	2.114636738614652
5	0.45312472705055645	1.613794177249216
6	0.2829644684585875	1.2093263977163764
7	0.21644338115184722	1.079201964264503
8	0.17755959465500015	1.0117996140590213
9	0.14354409558011255	0.9202126947261481
>10	0.80353476324471	10.938831786609889
>50	0.05265462391906944	2.5840244555820644
>100	0.02442740284905283	2.603743929955053
>500	0.0010856623488467926	0.5088397700951663
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	743	0.2874675003095209	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	573	0.22169431719697907	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	337	0.13038566299368579	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	267	0.10330258759440386	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.8690107713259874E-4	0.0	0.0	0.0	0.0
3	3.8690107713259874E-4	0.0	0.0	3.8690107713259874E-4	0.0
4	3.8690107713259874E-4	0.0	3.8690107713259874E-4	3.8690107713259874E-4	0.0
5	3.8690107713259874E-4	0.0	0.0011607032313977962	3.8690107713259874E-4	0.0
6	3.8690107713259874E-4	0.0	0.0023214064627955925	3.8690107713259874E-4	0.0
7	3.8690107713259874E-4	0.0	0.005803516156988981	7.738021542651975E-4	0.0
8	3.8690107713259874E-4	0.0	0.005803516156988981	7.738021542651975E-4	0.0
9	3.8690107713259874E-4	0.0	0.006964219388386777	0.0011607032313977962	0.0
10	7.738021542651975E-4	0.0	0.008124922619784574	0.001547604308530395	0.0
11	7.738021542651975E-4	0.0	0.011220131236845364	0.001547604308530395	0.0
12	7.738021542651975E-4	0.0	0.011607032313977961	0.001547604308530395	0.0
13	7.738021542651975E-4	0.0	0.01238083446824316	0.001547604308530395	0.0
14	7.738021542651975E-4	0.0	0.012767735545375759	0.001547604308530395	0.0
15	7.738021542651975E-4	0.0	0.013541537699640956	0.001547604308530395	0.0
16	7.738021542651975E-4	0.0	0.014315339853906154	0.001547604308530395	0.0
17	7.738021542651975E-4	0.0	0.014702240931038751	0.001547604308530395	0.0
18	7.738021542651975E-4	0.0	0.014702240931038751	0.001547604308530395	0.0
19	7.738021542651975E-4	0.0	0.014702240931038751	0.001547604308530395	0.0
20	7.738021542651975E-4	0.0	0.014702240931038751	0.001547604308530395	3.8690107713259874E-4
21	7.738021542651975E-4	0.0	0.01547604308530395	0.0019345053856629937	3.8690107713259874E-4
22	7.738021542651975E-4	0.0	0.01547604308530395	0.0023214064627955925	3.8690107713259874E-4
23	7.738021542651975E-4	0.0	0.01547604308530395	0.0023214064627955925	3.8690107713259874E-4
24	7.738021542651975E-4	0.0	0.01547604308530395	0.002708307539928191	3.8690107713259874E-4
25	7.738021542651975E-4	0.0	0.01547604308530395	0.0034821096941933887	3.8690107713259874E-4
26	0.0011607032313977962	0.0	0.01547604308530395	0.0034821096941933887	3.8690107713259874E-4
27	0.0011607032313977962	0.0	0.015862944162436547	0.004255911848458586	3.8690107713259874E-4
28	0.0011607032313977962	0.0	0.015862944162436547	0.010446329082580166	3.8690107713259874E-4
29	0.0011607032313977962	0.0	0.015862944162436547	0.023600965705088524	3.8690107713259874E-4
30	0.0011607032313977962	0.0	0.015862944162436547	0.03946390986752507	3.8690107713259874E-4
31	0.0011607032313977962	0.0	0.015862944162436547	0.09788597251454748	3.8690107713259874E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	30	3.5918294E-4	30.833334	5
GGTATCA	145	0.0	29.344828	1
TAACGGC	55	6.2302206E-7	26.90909	36
AACGGCC	55	6.2302206E-7	26.90909	37
TTAACGG	55	6.2302206E-7	26.90909	35
CCGCCCT	40	0.0019271608	23.125002	15
GTATCAA	445	0.0	22.44944	1
TTTAACG	80	6.917253E-7	20.8125	34
ATAGCTG	45	0.0038179087	20.555555	5
CTGTGAA	55	5.1282824E-4	20.181818	9
CCGCTCT	60	9.2104304E-4	18.5	28
TATTAGA	120	5.118636E-9	18.5	2
TTTATAC	60	9.2104304E-4	18.5	3
ACTAGAA	50	0.0070204977	18.499998	28
TCATATC	50	0.0070204977	18.499998	3
GTTTAAC	95	3.584555E-6	17.526316	33
CTCTAAT	65	0.0015754582	17.076923	1
TTATACT	110	7.7501863E-7	16.818182	4
ACTGCTC	125	1.6430386E-7	16.28	8
TAAACAA	70	0.0025852104	15.857142	5
>>END_MODULE