FastQCFastQC Report
Fri 10 Feb 2017
ERR1632972.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632972.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439801
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17080.38835746167016444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11970.27216854895736936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6570.14938574491645085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3300.026.909091
GTATCAA9250.023.81
CCGCTCT450.003822108720.55555728
TTCGCCG450.003822108720.55555724
AAGCGAA1001.2843884E-820.3521
TAGGGGT555.1361584E-420.1818184
TAAGACT1101.7462298E-920.1818184
CGAACTA951.6702506E-719.47368424
AAAGCGA1153.0358933E-919.30434620
GTCGCCC609.2244585E-418.537
ACGCATC500.007028145218.510
GCGAACT1002.8649083E-718.523
GCTAGCC801.6129748E-518.529
CCGCCGT609.2244585E-418.524
AGTACGA500.007028145218.55
CAAAGCG1103.835521E-818.519
TATACTG1054.7806316E-717.6190475
CATATAC852.716931E-517.4117643
TATATAC1401.8590072E-917.1785723
TATAGGA650.001577838217.0769232
Produced by FastQC (version 0.11.5)