##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632972.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	439801
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75800191450224	33.0	33.0	33.0	27.0	33.0
2	31.251288651003524	33.0	33.0	33.0	27.0	33.0
3	31.686676474132618	33.0	33.0	33.0	27.0	33.0
4	31.827208214624342	33.0	33.0	33.0	27.0	33.0
5	31.934297557304326	33.0	33.0	33.0	33.0	33.0
6	34.8026925814175	37.0	37.0	37.0	27.0	37.0
7	34.96486820175488	37.0	37.0	37.0	27.0	37.0
8	35.123985620769396	37.0	37.0	37.0	33.0	37.0
9	35.22776437525153	37.0	37.0	37.0	33.0	37.0
10	35.24415133207974	37.0	37.0	37.0	33.0	37.0
11	35.271825211857184	37.0	37.0	37.0	33.0	37.0
12	35.234631117255304	37.0	37.0	37.0	33.0	37.0
13	35.2632667956644	37.0	37.0	37.0	33.0	37.0
14	35.21085672838397	37.0	37.0	37.0	33.0	37.0
15	35.25177296095279	37.0	37.0	37.0	33.0	37.0
16	35.261179487995705	37.0	37.0	37.0	33.0	37.0
17	35.2208180518007	37.0	37.0	37.0	33.0	37.0
18	35.2237739341202	37.0	37.0	37.0	33.0	37.0
19	35.22666387752643	37.0	37.0	37.0	33.0	37.0
20	35.22737556303874	37.0	37.0	37.0	33.0	37.0
21	35.23252561954156	37.0	37.0	37.0	33.0	37.0
22	35.24160699952933	37.0	37.0	37.0	33.0	37.0
23	35.23531779145568	37.0	37.0	37.0	33.0	37.0
24	35.2170618074993	37.0	37.0	37.0	33.0	37.0
25	35.204331049724765	37.0	37.0	37.0	33.0	37.0
26	35.17683452288649	37.0	37.0	37.0	33.0	37.0
27	35.127580428421034	37.0	37.0	37.0	33.0	37.0
28	35.09754639029925	37.0	37.0	37.0	33.0	37.0
29	35.13017933110657	37.0	37.0	37.0	33.0	37.0
30	35.08339680901135	37.0	37.0	37.0	33.0	37.0
31	35.07811714843759	37.0	37.0	37.0	33.0	37.0
32	35.11909022489717	37.0	37.0	37.0	33.0	37.0
33	35.10160731785512	37.0	37.0	37.0	33.0	37.0
34	35.01159842747061	37.0	37.0	37.0	27.0	37.0
35	35.02600266938911	37.0	37.0	37.0	27.0	37.0
36	34.99486813354221	37.0	37.0	37.0	27.0	37.0
37	35.020695723747785	37.0	37.0	37.0	27.0	37.0
38	34.88638043114954	37.0	37.0	37.0	27.0	37.0
39	34.94645987617127	37.0	37.0	37.0	27.0	37.0
40	34.97350847315035	37.0	37.0	37.0	27.0	37.0
41	34.99366304305811	37.0	37.0	37.0	27.0	37.0
42	34.915275317700505	37.0	37.0	37.0	27.0	37.0
43	34.68811576144665	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	14.0
16	26.0
17	27.0
18	21.0
19	24.0
20	34.0
21	90.0
22	259.0
23	674.0
24	1304.0
25	2226.0
26	3791.0
27	5507.0
28	7914.0
29	10522.0
30	13709.0
31	17070.0
32	21934.0
33	28297.0
34	40354.0
35	73487.0
36	212517.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.80754704968838	20.772121936966947	13.891737399414735	24.528593613929935
2	15.469723806903577	23.550196566174247	38.95557308873786	22.024506538184315
3	17.644343691806068	27.18752344810494	31.211388787201482	23.95674407288751
4	12.587056418698456	18.386952280690586	39.6836296415879	29.34236165902306
5	13.424935368496207	37.71865002580713	35.62724959697681	13.229165008719853
6	28.63090352227485	40.42601085490938	17.808508848320034	13.134576774495738
7	25.617267809759415	32.93239442384169	23.98903140283901	17.461306363559885
8	23.81577122380349	36.71410478830198	21.078396820380128	18.3917271675144
9	25.87738545387573	15.828977196504782	20.556342527643185	37.737294821976306
10	14.537029247318673	28.741408045911676	34.51629259596954	22.205270110800114
11	33.111793743079254	24.22891262184488	24.62295447259101	18.03633916248485
12	22.40968074197194	26.259603775343848	30.86032091786967	20.47039456481454
13	27.662056248166785	22.627506531363046	26.94423159565349	22.766205624816678
14	21.799859481902043	21.57180179217419	28.364191986830406	28.264146739093366
15	23.635917153439852	29.07655962583077	25.07565921860114	22.211864002128234
16	22.275301784216044	29.157505326272563	25.594757629018577	22.972435260492812
17	22.004724864199947	28.00152796378362	26.818038158166992	23.175709013849445
18	22.58748843226823	27.622720275761083	28.219808504300808	21.56998278766988
19	24.375342484441827	26.92626892617343	27.978108280790632	20.720280308594113
20	24.02313773729482	26.63045331866003	28.109986107353098	21.23642283669205
21	22.392627574744033	27.7173085099852	27.707986111900613	22.18207780337016
22	22.13751219301457	27.612488375424338	27.769377513921068	22.480621917640022
23	22.18071354999193	27.42649516485865	28.200481581442517	22.192309703706904
24	22.910589107346276	27.708895614152762	27.150461231329626	22.230054047171336
25	22.707542729552685	27.26892389967281	28.030859411415616	21.992673959358893
26	22.795764448011717	27.382839056755216	27.673197650755682	22.14819884447739
27	22.73869318168899	27.327132043810725	27.347595844484214	22.586578930016074
28	22.05974975045532	27.50789561642652	28.18183678527334	22.25051784784482
29	23.048151322984715	27.606576610785332	27.367377518468576	21.977894547761373
30	22.599084585983206	27.528586792663045	27.76437525153422	22.107953369819533
31	22.700039335972406	27.327814170499842	27.80894086188981	22.163205631637943
32	21.76370676737888	27.177518923331235	28.141591310615482	22.917182998674402
33	22.0056343664521	27.082703313544084	28.562463477800186	22.349198842203634
34	22.271436399644383	27.069970282013912	28.11862637874857	22.539966939593135
35	22.34601558432109	27.597026837137705	27.779154663131735	22.27780291540947
36	22.76211286468198	27.541092448630177	27.504257607417898	22.192537079269943
37	22.822367388887248	26.929679559619007	27.532224801671667	22.715728249822078
38	22.815091370870007	27.343957835475592	27.560191995925432	22.28075879772897
39	22.440376442982167	27.085431820300542	27.9474125797804	22.52677915693689
40	22.25574748579471	27.577927289842453	28.027903529096115	22.13842169526672
41	22.290763322502677	26.910580012323752	28.10452909384017	22.694127571333397
42	22.372163774070543	27.273244035370542	27.752324346693165	22.602267843865746
43	22.465615130479467	26.599302866523722	28.076561899586405	22.858520103410406
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	15.5
2	19.0
3	95.5
4	172.0
5	172.0
6	220.0
7	268.0
8	247.0
9	226.0
10	330.0
11	434.0
12	434.0
13	706.0
14	978.0
15	1709.0
16	2440.0
17	2839.0
18	3238.0
19	3238.0
20	3186.0
21	3134.0
22	3793.5
23	4453.0
24	5817.0
25	7181.0
26	7181.0
27	9174.0
28	11167.0
29	14053.5
30	16940.0
31	18791.0
32	20642.0
33	20642.0
34	22800.5
35	24959.0
36	26970.0
37	28981.0
38	30551.0
39	32121.0
40	32121.0
41	33555.5
42	34990.0
43	34080.5
44	33171.0
45	32762.0
46	32353.0
47	32353.0
48	31740.0
49	31127.0
50	31189.5
51	31252.0
52	30564.0
53	29876.0
54	29876.0
55	27176.5
56	24477.0
57	21674.5
58	18872.0
59	16612.5
60	14353.0
61	14353.0
62	12675.5
63	10998.0
64	9095.5
65	7193.0
66	6155.0
67	5117.0
68	5117.0
69	4172.0
70	3227.0
71	2752.5
72	2278.0
73	1858.5
74	1439.0
75	1439.0
76	1082.0
77	725.0
78	579.0
79	433.0
80	324.0
81	215.0
82	215.0
83	182.0
84	149.0
85	127.0
86	105.0
87	81.0
88	57.0
89	57.0
90	39.5
91	22.0
92	13.0
93	4.0
94	3.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	439801.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.5222655629958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.36419666750054	61.015952847697505
2	5.5390100113917855	7.48013009890577
3	1.3641803642052195	2.7633764648306745
4	0.6441550949423527	1.7397924553781725
5	0.38244669959798777	1.2911833806973303
6	0.2452628233129305	0.9936420893079485
7	0.186580277158617	0.8818826116185046
8	0.1439412170315384	0.7775389665491479
9	0.11472894577285377	0.6972082509813458
>10	0.8674602582715409	11.91787492839323
>50	0.10061139436544639	4.582530810214355
>100	0.046409969739601466	5.044123457028816
>500	3.387589032087698E-4	0.15028065986159675
>1k	6.775178064175396E-4	0.6644829785356751
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1708	0.38835746167016444	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1197	0.27216854895736936	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	657	0.14938574491645085	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	6.821266891162139E-4	0.0	0.0
2	2.2737556303873799E-4	0.0	6.821266891162139E-4	0.0	0.0
3	2.2737556303873799E-4	0.0	6.821266891162139E-4	0.0	0.0
4	2.2737556303873799E-4	0.0	6.821266891162139E-4	2.2737556303873799E-4	0.0
5	4.5475112607747597E-4	0.0	0.0011368778151936898	2.2737556303873799E-4	0.0
6	4.5475112607747597E-4	0.0	0.0027285067564648555	2.2737556303873799E-4	0.0
7	4.5475112607747597E-4	0.0	0.0050022623868522355	2.2737556303873799E-4	0.0
8	4.5475112607747597E-4	0.0	0.005684389075968449	2.2737556303873799E-4	0.0
9	4.5475112607747597E-4	0.0	0.006593891328123401	4.5475112607747597E-4	0.0
10	0.0018190045043099039	0.0	0.0072760180172396155	4.5475112607747597E-4	0.0
11	0.0018190045043099039	0.0	0.011596153714975637	4.5475112607747597E-4	0.0
12	0.0018190045043099039	0.0	0.012960407093208065	6.821266891162139E-4	0.0
13	0.0018190045043099039	0.0	0.013869909345363016	6.821266891162139E-4	0.0
14	0.0018190045043099039	0.0	0.014552036034479231	6.821266891162139E-4	0.0
15	0.0018190045043099039	0.0	0.015461538286634182	6.821266891162139E-4	0.0
16	0.002046380067348642	0.0	0.017280542790944087	6.821266891162139E-4	0.0
17	0.002046380067348642	0.0	0.018417420606137776	6.821266891162139E-4	0.0
18	0.002046380067348642	0.0	0.018644796169176515	6.821266891162139E-4	0.0
19	0.002046380067348642	0.0	0.01909954729525399	6.821266891162139E-4	0.0
20	0.002046380067348642	0.0	0.01932692285829273	6.821266891162139E-4	0.0
21	0.002046380067348642	0.0	0.020009049547408942	0.0011368778151936898	0.0
22	0.002046380067348642	0.0	0.02023642511044768	0.0011368778151936898	0.0
23	0.002046380067348642	0.0	0.02023642511044768	0.0011368778151936898	0.0
24	0.002046380067348642	0.0	0.02023642511044768	0.0011368778151936898	0.0
25	0.002046380067348642	0.0	0.020463800673486417	0.0013642533782324277	0.0
26	0.002046380067348642	0.0	0.020463800673486417	0.0018190045043099039	0.0
27	0.002046380067348642	0.0	0.020463800673486417	0.0029558823195035937	0.0
28	0.002046380067348642	0.0	0.020463800673486417	0.009549773647626995	0.0
29	0.002046380067348642	0.0	0.020463800673486417	0.026602940875532343	0.0
30	0.002046380067348642	0.0	0.020463800673486417	0.045702488170786336	0.0
31	0.002046380067348642	0.0	0.020463800673486417	0.11891741946925996	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	330	0.0	26.90909	1
GTATCAA	925	0.0	23.8	1
CCGCTCT	45	0.0038221087	20.555557	28
TTCGCCG	45	0.0038221087	20.555557	24
AAGCGAA	100	1.2843884E-8	20.35	21
TAGGGGT	55	5.1361584E-4	20.181818	4
TAAGACT	110	1.7462298E-9	20.181818	4
CGAACTA	95	1.6702506E-7	19.473684	24
AAAGCGA	115	3.0358933E-9	19.304346	20
GTCGCCC	60	9.2244585E-4	18.5	37
ACGCATC	50	0.0070281452	18.5	10
GCGAACT	100	2.8649083E-7	18.5	23
GCTAGCC	80	1.6129748E-5	18.5	29
CCGCCGT	60	9.2244585E-4	18.5	24
AGTACGA	50	0.0070281452	18.5	5
CAAAGCG	110	3.835521E-8	18.5	19
TATACTG	105	4.7806316E-7	17.619047	5
CATATAC	85	2.716931E-5	17.411764	3
TATATAC	140	1.8590072E-9	17.178572	3
TATAGGA	65	0.0015778382	17.076923	2
>>END_MODULE