FastQCFastQC Report
Fri 10 Feb 2017
ERR1632971.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632971.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323738
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10510.3246452378157646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7820.24155335487338528No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC6150.18996843126231705No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4490.13869239940939895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGCC250.00549058429.635
ACATCGC250.00549058429.66
GGTATCA2650.027.9245281
TAATAGT400.001928207723.1254
CTACTAG400.001928207723.1251
CACGGTA400.001928207723.1252
GACCTTC502.6962953E-422.27
AGTTCGC603.7156504E-521.5833348
TCTATAC806.928385E-720.81253
TTAGAAC555.132132E-420.1818183
ATTACCC555.132132E-420.1818183
GCTTCGC555.132132E-420.18181822
TTGCCGA555.132132E-420.18181810
GTATCAA5900.019.1271211
ACTATTC1002.8591057E-718.53
GTCGCCC701.21576726E-418.537
GGTGTTA609.217287E-418.58
GAGAGCA609.217287E-418.59
TCGCTTT701.21576726E-418.511
CCGGTCT852.7133437E-517.41176424
Produced by FastQC (version 0.11.5)