##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632971.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	323738
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.635887662245395	33.0	33.0	33.0	27.0	33.0
2	31.173652768596828	33.0	33.0	33.0	27.0	33.0
3	31.5932575107031	33.0	33.0	33.0	27.0	33.0
4	31.7268068623393	33.0	33.0	33.0	27.0	33.0
5	31.83138216706102	33.0	33.0	33.0	33.0	33.0
6	34.69097850731147	37.0	37.0	37.0	27.0	37.0
7	34.83186403820373	37.0	37.0	37.0	27.0	37.0
8	35.04138222883937	37.0	37.0	37.0	33.0	37.0
9	35.07862221920195	37.0	37.0	37.0	33.0	37.0
10	35.15566909043733	37.0	37.0	37.0	33.0	37.0
11	35.19178471480024	37.0	37.0	37.0	33.0	37.0
12	35.10320691423311	37.0	37.0	37.0	33.0	37.0
13	35.17069358555375	37.0	37.0	37.0	33.0	37.0
14	35.114086081955165	37.0	37.0	37.0	33.0	37.0
15	35.152802574921694	37.0	37.0	37.0	33.0	37.0
16	35.15679345643699	37.0	37.0	37.0	33.0	37.0
17	35.12117823672229	37.0	37.0	37.0	33.0	37.0
18	35.104010032804304	37.0	37.0	37.0	33.0	37.0
19	35.11382661287831	37.0	37.0	37.0	33.0	37.0
20	35.057747314186166	37.0	37.0	37.0	27.0	37.0
21	35.12754758477534	37.0	37.0	37.0	33.0	37.0
22	35.123955173628055	37.0	37.0	37.0	33.0	37.0
23	35.11423434999907	37.0	37.0	37.0	33.0	37.0
24	35.10765495555047	37.0	37.0	37.0	33.0	37.0
25	35.116399681223704	37.0	37.0	37.0	33.0	37.0
26	35.086208600782115	37.0	37.0	37.0	33.0	37.0
27	35.01179039840859	37.0	37.0	37.0	27.0	37.0
28	35.004617931784345	37.0	37.0	37.0	27.0	37.0
29	35.03406458308879	37.0	37.0	37.0	27.0	37.0
30	35.00034904768671	37.0	37.0	37.0	27.0	37.0
31	34.9906652910687	37.0	37.0	37.0	27.0	37.0
32	35.00799411870093	37.0	37.0	37.0	27.0	37.0
33	34.975381326875436	37.0	37.0	37.0	27.0	37.0
34	34.93386936349764	37.0	37.0	37.0	27.0	37.0
35	34.92905682990566	37.0	37.0	37.0	27.0	37.0
36	34.896135146322024	37.0	37.0	37.0	27.0	37.0
37	34.92824135566415	37.0	37.0	37.0	27.0	37.0
38	34.794064953758905	37.0	37.0	37.0	27.0	37.0
39	34.850351209929016	37.0	37.0	37.0	27.0	37.0
40	34.89023840265894	37.0	37.0	37.0	27.0	37.0
41	34.933313358332974	37.0	37.0	37.0	27.0	37.0
42	34.779482173856636	37.0	37.0	37.0	27.0	37.0
43	34.61138327907135	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	10.0
16	15.0
17	15.0
18	6.0
19	12.0
20	35.0
21	65.0
22	216.0
23	463.0
24	1019.0
25	1809.0
26	2964.0
27	4325.0
28	6172.0
29	8100.0
30	10591.0
31	13524.0
32	17218.0
33	21962.0
34	31358.0
35	56646.0
36	147212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.62328178959529	20.413111837349955	13.501658748741265	25.461947624313485
2	16.294658025934552	22.644545898226344	38.06071576398198	23.00008031185712
3	17.89718846721732	27.001773038691784	30.032001186144353	25.06903730794655
4	13.038321111516101	18.011169525974708	39.127010113116164	29.823499249393027
5	14.152493683163545	37.11241806646115	35.1086372313414	13.626451019033912
6	29.77191432578196	39.43868189708962	17.527136140953488	13.262267636174933
7	26.614113882213395	31.92365431305562	24.008611902217226	17.45361990251376
8	24.577281628971576	35.92225812230878	20.67814096584275	18.822319282876894
9	26.124211553787326	15.605829405259808	20.042441727569827	38.227517313383046
10	15.165040866379604	28.023278082894194	34.10257677504649	22.709104275679717
11	33.8653479047872	23.973398241788114	23.986989479146718	18.174264374277964
12	23.295071940890473	25.69176309237717	29.910606725191357	21.102558241541
13	27.972002051041272	21.86521199241362	26.17826761146359	23.984518345081515
14	22.224453107142196	20.866873830072468	27.517622274802463	29.391050787982877
15	23.949922468168705	28.41835064156818	24.272714355435568	23.359012534827546
16	23.012435982183124	28.01771803124749	25.056990529378698	23.91285545719069
17	22.3798256614917	27.50773773854166	26.415805373481028	23.696631226485614
18	23.287967430453023	26.954203707936664	27.37120758143931	22.386621280171003
19	24.7845479986903	26.08374673346966	26.95543927496927	22.17626599287078
20	24.216187163694098	26.35680704767435	27.074980385373358	22.352025403258192
21	23.68180442209441	26.88532084586919	26.859065046426434	22.57380968560997
22	23.272831734303665	26.491483854227805	26.931345717833555	23.30433869363498
23	23.358085859553096	26.445150090505287	27.42649920614818	22.770264843793438
24	23.111590236549308	27.126874200742574	26.46213913720354	23.299396425504575
25	23.46032903150078	26.54121542728997	27.438545984716033	22.559909556493214
26	23.34233237988744	26.758984116785793	27.515151140737267	22.383532362589502
27	23.50202941885105	26.68176117724827	26.388622898763813	23.42758650513687
28	22.816289715757804	26.293175345495435	27.836707460971528	23.053827477775236
29	24.039192186274086	26.250548282870717	26.972737213425674	22.737522317429526
30	23.67593547868956	26.276186298797178	27.1923592534704	22.85551896904287
31	23.304956477151276	26.184754338384742	27.626043281913155	22.88424590255083
32	22.699219739418915	26.23263256089801	27.45121054680019	23.616937152882887
33	22.98401794043331	26.663845455275563	27.264639924877525	23.0874966794136
34	23.34974578208304	26.397889651508315	27.16270564468799	23.089658921720652
35	23.423879804039068	26.814893525010962	27.16981015512544	22.591416515824527
36	24.147613193384775	26.753424065139093	26.208539003762297	22.89042373771383
37	23.303103126602377	26.196492225194447	27.072818143066307	23.42758650513687
38	23.839957002267266	26.62677844429755	26.636662980558352	22.896601572876833
39	22.86231458772217	26.671567749229315	27.28379121388283	23.182326449165682
40	23.156070649722924	26.60392045419444	27.641796761578806	22.59821213450383
41	22.877759175629674	25.89254273517474	27.59484521433999	23.634852874855593
42	22.54322940155311	27.09104275679716	27.00022857990103	23.3654992617487
43	23.20518443926879	25.819644280251314	27.746202175833545	23.22896910464635
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	15.0
2	17.0
3	58.5
4	100.0
5	100.0
6	130.5
7	161.0
8	169.5
9	178.0
10	199.5
11	221.0
12	221.0
13	393.0
14	565.0
15	1023.0
16	1481.0
17	1621.0
18	1761.0
19	1761.0
20	1725.0
21	1689.0
22	1936.5
23	2184.0
24	2948.5
25	3713.0
26	3713.0
27	4907.5
28	6102.0
29	7739.5
30	9377.0
31	10768.5
32	12160.0
33	12160.0
34	14329.0
35	16498.0
36	18349.0
37	20200.0
38	21778.5
39	23357.0
40	23357.0
41	24749.5
42	26142.0
43	26501.5
44	26861.0
45	26628.0
46	26395.0
47	26395.0
48	25352.5
49	24310.0
50	24012.0
51	23714.0
52	22598.0
53	21482.0
54	21482.0
55	20527.0
56	19572.0
57	17175.5
58	14779.0
59	13115.5
60	11452.0
61	11452.0
62	10585.0
63	9718.0
64	7748.0
65	5778.0
66	4788.5
67	3799.0
68	3799.0
69	3257.0
70	2715.0
71	2397.5
72	2080.0
73	2129.5
74	2179.0
75	2179.0
76	2016.5
77	1854.0
78	1281.5
79	709.0
80	445.0
81	181.0
82	181.0
83	144.0
84	107.0
85	94.0
86	81.0
87	58.0
88	35.0
89	35.0
90	24.5
91	14.0
92	8.5
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	323738.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.53161259624967
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.46223504061315	54.4322397591739
2	5.869108440227813	7.2227141365895395
3	1.661984639336061	3.0679378490563276
4	0.8401438114936677	2.0678161413585987
5	0.5378512481992239	1.6547427319301988
6	0.3940117001015997	1.4546505173424806
7	0.2887648721533008	1.243771777131973
8	0.228014160356541	1.1224063185214266
9	0.17351339724921366	0.9608903225818008
>10	1.3674469603270658	16.44307299024392
>50	0.13294704849956768	5.5054714535512055
>100	0.04246217518617363	4.062851306569817
>500	0.0010110041710993721	0.43452788817011434
>1k	5.055020855496861E-4	0.3269068077786616
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1051	0.3246452378157646	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	782	0.24155335487338528	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	615	0.18996843126231705	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	449	0.13869239940939895	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.0889175815010904E-4	0.0	0.0
2	0.0	0.0	3.0889175815010904E-4	0.0	0.0
3	0.0	0.0	3.0889175815010904E-4	0.0	0.0
4	0.0	0.0	6.177835163002181E-4	0.0	0.0
5	0.0	0.0	6.177835163002181E-4	0.0	0.0
6	0.0	0.0	0.0015444587907505452	0.0	0.0
7	0.0	0.0	0.0027800258233509813	0.0	0.0
8	0.0	0.0	0.0030889175815010904	0.0	0.0
9	0.0	0.0	0.0033978093396511994	0.0012355670326004362	0.0
10	3.0889175815010904E-4	0.0	0.0040155928559514175	0.0012355670326004362	0.0
11	3.0889175815010904E-4	0.0	0.007413402195602617	0.0012355670326004362	0.0
12	3.0889175815010904E-4	0.0	0.008031185711902835	0.0012355670326004362	0.0
13	6.177835163002181E-4	0.0	0.008648969228203053	0.0012355670326004362	0.0
14	6.177835163002181E-4	0.0	0.009266752744503271	0.0012355670326004362	0.0
15	6.177835163002181E-4	0.0	0.011120103293403925	0.0012355670326004362	0.0
16	6.177835163002181E-4	0.0	0.012355670326004362	0.0012355670326004362	0.0
17	6.177835163002181E-4	0.0	0.01297345384230458	0.0012355670326004362	0.0
18	6.177835163002181E-4	0.0	0.013900129116754907	0.0012355670326004362	0.0
19	6.177835163002181E-4	0.0	0.014517912633055125	0.0015444587907505452	0.0
20	6.177835163002181E-4	0.0	0.015444587907505452	0.0015444587907505452	0.0
21	6.177835163002181E-4	0.0	0.01637126318195578	0.0018533505489006542	0.0
22	6.177835163002181E-4	0.0	0.016989046698256	0.0018533505489006542	0.0
23	6.177835163002181E-4	0.0	0.017297938456406106	0.0024711340652008723	0.0
24	6.177835163002181E-4	0.0	0.017297938456406106	0.0024711340652008723	0.0
25	6.177835163002181E-4	0.0	0.017297938456406106	0.0027800258233509813	0.0
26	6.177835163002181E-4	0.0	0.017606830214556217	0.0030889175815010904	0.0
27	6.177835163002181E-4	0.0	0.017606830214556217	0.0033978093396511994	0.0
28	6.177835163002181E-4	0.0	0.017606830214556217	0.00957564450265338	0.0
29	6.177835163002181E-4	0.0	0.017606830214556217	0.021931314828657743	0.0
30	6.177835163002181E-4	0.0	0.017606830214556217	0.041082603833964504	0.0
31	6.177835163002181E-4	0.0	0.017606830214556217	0.09112306865428217	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGCC	25	0.005490584	29.6	35
ACATCGC	25	0.005490584	29.6	6
GGTATCA	265	0.0	27.924528	1
TAATAGT	40	0.0019282077	23.125	4
CTACTAG	40	0.0019282077	23.125	1
CACGGTA	40	0.0019282077	23.125	2
GACCTTC	50	2.6962953E-4	22.2	7
AGTTCGC	60	3.7156504E-5	21.583334	8
TCTATAC	80	6.928385E-7	20.8125	3
TTAGAAC	55	5.132132E-4	20.181818	3
ATTACCC	55	5.132132E-4	20.181818	3
GCTTCGC	55	5.132132E-4	20.181818	22
TTGCCGA	55	5.132132E-4	20.181818	10
GTATCAA	590	0.0	19.127121	1
ACTATTC	100	2.8591057E-7	18.5	3
GTCGCCC	70	1.21576726E-4	18.5	37
GGTGTTA	60	9.217287E-4	18.5	8
GAGAGCA	60	9.217287E-4	18.5	9
TCGCTTT	70	1.21576726E-4	18.5	11
CCGGTCT	85	2.7133437E-5	17.411764	24
>>END_MODULE