##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632970.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	363288
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80548490453855	33.0	33.0	33.0	33.0	33.0
2	31.29918687102244	33.0	33.0	33.0	27.0	33.0
3	31.740569465547996	33.0	33.0	33.0	27.0	33.0
4	31.849565633877255	33.0	33.0	33.0	33.0	33.0
5	31.9632440377882	33.0	33.0	33.0	33.0	33.0
6	34.85196593336416	37.0	37.0	37.0	27.0	37.0
7	35.03711931029927	37.0	37.0	37.0	33.0	37.0
8	35.20665973002136	37.0	37.0	37.0	33.0	37.0
9	35.29362654422937	37.0	37.0	37.0	33.0	37.0
10	35.31562561934333	37.0	37.0	37.0	33.0	37.0
11	35.348508621259164	37.0	37.0	37.0	33.0	37.0
12	35.31364372068442	37.0	37.0	37.0	33.0	37.0
13	35.3488582061615	37.0	37.0	37.0	33.0	37.0
14	35.311149831538614	37.0	37.0	37.0	33.0	37.0
15	35.32780328554755	37.0	37.0	37.0	33.0	37.0
16	35.34956838651428	37.0	37.0	37.0	33.0	37.0
17	35.306387769483166	37.0	37.0	37.0	33.0	37.0
18	35.30238543524697	37.0	37.0	37.0	33.0	37.0
19	35.3122591442602	37.0	37.0	37.0	33.0	37.0
20	35.31690284292352	37.0	37.0	37.0	33.0	37.0
21	35.320007817489156	37.0	37.0	37.0	33.0	37.0
22	35.31443372751095	37.0	37.0	37.0	33.0	37.0
23	35.29544053202969	37.0	37.0	37.0	33.0	37.0
24	35.29727103565215	37.0	37.0	37.0	33.0	37.0
25	35.29256402633723	37.0	37.0	37.0	33.0	37.0
26	35.26625432164013	37.0	37.0	37.0	33.0	37.0
27	35.208005769527205	37.0	37.0	37.0	33.0	37.0
28	35.17557144744666	37.0	37.0	37.0	33.0	37.0
29	35.20753506859572	37.0	37.0	37.0	33.0	37.0
30	35.19387097839731	37.0	37.0	37.0	33.0	37.0
31	35.172964700182774	37.0	37.0	37.0	33.0	37.0
32	35.19387923630838	37.0	37.0	37.0	33.0	37.0
33	35.17756986192773	37.0	37.0	37.0	33.0	37.0
34	35.12448250423906	37.0	37.0	37.0	33.0	37.0
35	35.09782871991368	37.0	37.0	37.0	33.0	37.0
36	35.078271233842024	37.0	37.0	37.0	33.0	37.0
37	35.10490574970822	37.0	37.0	37.0	33.0	37.0
38	34.964460703353815	37.0	37.0	37.0	27.0	37.0
39	35.02572064037348	37.0	37.0	37.0	27.0	37.0
40	35.05407830701812	37.0	37.0	37.0	27.0	37.0
41	35.070007817489156	37.0	37.0	37.0	33.0	37.0
42	34.98126279976217	37.0	37.0	37.0	27.0	37.0
43	34.76939508048711	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	11.0
16	20.0
17	18.0
18	11.0
19	19.0
20	26.0
21	58.0
22	172.0
23	458.0
24	856.0
25	1644.0
26	2789.0
27	4239.0
28	6066.0
29	8054.0
30	10923.0
31	13939.0
32	17795.0
33	23163.0
34	32714.0
35	61245.0
36	179068.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.78940124639405	20.757085287705625	14.252879258329479	24.200634207570854
2	15.235295413005659	23.97299112549823	39.14387483208914	21.64783862940697
3	17.603939574111998	27.491962299883287	31.645966836229107	23.258131289775605
4	12.658276629010592	18.366970557794367	39.51355398471736	29.46119882847768
5	13.482691418378806	37.642311334258224	35.474609676069676	13.4003875712933
6	28.266829622778623	40.55597762656625	17.9521481579353	13.225044592719826
7	24.99421946224483	33.39306555680342	24.004371187597716	17.608343793354035
8	23.562297681178567	36.56300235625729	21.286692651560195	18.588007311003942
9	25.712657726101607	15.979057937504129	20.392911409129947	37.915372927264315
10	14.315364118825835	28.9112219506287	34.469071370372816	22.304342560172646
11	32.83813393230715	24.47589791019797	24.64490982361102	18.04105833388386
12	22.012563035387902	26.455319195789567	30.99744555283962	20.534672215982912
13	27.82420558895422	22.655028517319593	27.188621699588207	22.332144194137985
14	21.81795159763053	21.83143951905926	28.543194380216246	27.807414503093963
15	23.353647794587214	29.08326176477065	25.437669287177116	22.12542115346502
16	22.093215300257647	29.52175684305565	25.530157891259826	22.85486996542688
17	21.869150646319174	28.071117130210745	26.968961264891767	23.090770958578318
18	22.361597410319085	27.49086124507278	28.44464997467574	21.702891369932395
19	24.171456255092377	26.976393384862696	28.609257668846755	20.242892691198165
20	24.230637951157206	26.32154103631279	28.438869436920573	21.008951575609434
21	22.358569509590186	28.01936755411684	27.570412455132015	22.05165048116095
22	22.007883552443243	27.833839818546167	27.778787078020745	22.37948955098985
23	21.78684679923367	27.669232124375153	28.28141859901786	22.262502477373324
24	22.810552509303914	27.614454647552357	27.825306643764726	21.749686199379006
25	22.74201184734976	27.27670608442888	28.329865010680233	21.651417057541124
26	22.565843077668408	27.21229437801414	27.90540838122922	22.31645416308824
27	22.353064235537644	27.257437625244986	28.01496333487481	22.37453480434256
28	21.557001607540023	27.805487657175576	28.2574706568893	22.3800400783951
29	22.50886349122459	27.791724472044216	27.705291669419303	21.994120367311883
30	22.174142828830018	28.035883376274473	27.940366871462864	21.84960692343265
31	22.568595714694677	27.472693840699392	28.093413490123538	21.865296954482393
32	21.765926757834006	27.391215784721762	28.20709739930854	22.63576005813569
33	21.85153376935104	27.074387262997952	28.59604501112065	22.478033956530354
34	21.700964524014005	27.34552201008566	28.21948426592676	22.734029199973573
35	22.23249873378697	27.836317191869814	27.927980004844642	22.00320406949858
36	22.268283015128493	27.79695448239413	27.888342031666337	22.046420470811036
37	22.77834665609654	27.089251502939817	27.823379797846336	22.309022043117306
38	22.802019334522473	27.490585981370153	27.852007222919557	21.855387461187817
39	22.217359230142478	27.267347118539558	28.233247451058112	22.28204620025985
40	22.29140516614917	27.146231089383633	28.482361101935656	22.080002642531547
41	22.146065931162052	27.321298804254475	28.31169760630684	22.22093765827663
42	22.280394618044085	27.109345753231594	28.01496333487481	22.595296293849508
43	21.946224483054767	27.11017154433948	28.186727885314134	22.756876087291626
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16.0
1	21.0
2	26.0
3	95.0
4	164.0
5	164.0
6	219.0
7	274.0
8	256.0
9	238.0
10	336.5
11	435.0
12	435.0
13	661.5
14	888.0
15	1728.5
16	2569.0
17	2928.5
18	3288.0
19	3288.0
20	3124.5
21	2961.0
22	3545.0
23	4129.0
24	5224.0
25	6319.0
26	6319.0
27	7909.5
28	9500.0
29	12357.5
30	15215.0
31	16324.5
32	17434.0
33	17434.0
34	18875.0
35	20316.0
36	21617.5
37	22919.0
38	24376.0
39	25833.0
40	25833.0
41	27288.0
42	28743.0
43	27117.0
44	25491.0
45	25950.5
46	26410.0
47	26410.0
48	26099.5
49	25789.0
50	26293.0
51	26797.0
52	26773.0
53	26749.0
54	26749.0
55	23091.0
56	19433.0
57	17410.0
58	15387.0
59	13497.5
60	11608.0
61	11608.0
62	10095.0
63	8582.0
64	6994.5
65	5407.0
66	4616.5
67	3826.0
68	3826.0
69	3162.0
70	2498.0
71	2073.5
72	1649.0
73	1391.0
74	1133.0
75	1133.0
76	848.0
77	563.0
78	432.5
79	302.0
80	241.5
81	181.0
82	181.0
83	128.5
84	76.0
85	82.0
86	88.0
87	61.5
88	35.0
89	35.0
90	24.5
91	14.0
92	7.5
93	1.0
94	1.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	363288.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.59512110688574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.80934750614053	62.058639634217286
2	4.626629027283554	6.254750988317297
3	1.114343966217755	2.259726460536496
4	0.521005050719314	1.4086959800268473
5	0.32386890378548566	1.0945978887067116
6	0.22161025327178965	0.8987863145060547
7	0.1763279643559301	0.8343237073618809
8	0.13370240936035516	0.7230104442396498
9	0.09975463295153968	0.6068633845799033
>10	0.8003457850596777	10.86521773733254
>50	0.12016089751246432	5.750193521333838
>100	0.050853076080273056	5.935647865720606
>500	0.0012303163567807997	0.45823024108145716
>1k	8.202109045205332E-4	0.8513158320394163
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1774	0.48831780846050515	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1297	0.3570170223073705	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	603	0.16598401268415142	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	531	0.146165026094999	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	519	0.14286186166347362	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	464	0.12772235801898218	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	447	0.1230428750743212	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	394	0.10845389883508401	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	382	0.10515073440355861	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	377	0.10377441589042302	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	371	0.10212283367466031	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	8.257911078813503E-4	0.0	0.0
2	2.752637026271168E-4	0.0	8.257911078813503E-4	0.0	0.0
3	2.752637026271168E-4	0.0	0.0011010548105084672	0.0	0.0
4	2.752637026271168E-4	0.0	0.0013763185131355838	0.0	0.0
5	5.505274052542336E-4	0.0	0.0019268459183898175	0.0	0.0
6	5.505274052542336E-4	0.0	0.003853691836779635	0.0	0.0
7	8.257911078813503E-4	0.0	0.007982647376186387	2.752637026271168E-4	0.0
8	8.257911078813503E-4	0.0	0.00935896588932197	2.752637026271168E-4	0.0
9	8.257911078813503E-4	0.0	0.01101054810508467	8.257911078813503E-4	0.0
10	0.0019268459183898175	0.0	0.012111602915593139	0.0011010548105084672	0.0
11	0.0019268459183898175	0.0	0.019818986589152408	0.0011010548105084672	0.0
12	0.0019268459183898175	0.0	0.021745832507542227	0.0011010548105084672	0.0
13	0.0019268459183898175	0.0	0.023672678425932042	0.0011010548105084672	0.0
14	0.0019268459183898175	0.0	0.025048996939067626	0.0011010548105084672	0.0
15	0.0019268459183898175	0.0	0.027251106560084563	0.0013763185131355838	0.0
16	0.0019268459183898175	0.0	0.028627425073220143	0.0013763185131355838	0.0
17	0.0019268459183898175	0.0	0.029177952478474378	0.0013763185131355838	0.0
18	0.0019268459183898175	0.0	0.029453216181101496	0.0013763185131355838	0.0
19	0.0019268459183898175	0.0	0.029728479883728613	0.0013763185131355838	0.0
20	0.0019268459183898175	0.0	0.031104798396864197	0.0013763185131355838	0.0
21	0.0019268459183898175	0.0	0.032756380612626895	0.0016515822157627006	0.0
22	0.0019268459183898175	0.0	0.033857435423135365	0.0019268459183898175	0.0
23	0.0019268459183898175	0.0	0.0344079628283896	0.0019268459183898175	0.0
24	0.0019268459183898175	0.0	0.0344079628283896	0.0019268459183898175	0.0
25	0.0019268459183898175	0.0	0.0344079628283896	0.0019268459183898175	0.0
26	0.0019268459183898175	0.0	0.0344079628283896	0.0022021096210169343	0.0
27	0.0019268459183898175	0.0	0.0344079628283896	0.0030279007288982846	0.0
28	0.0019268459183898175	0.0	0.0344079628283896	0.012662130320847372	0.0
29	0.0019268459183898175	0.0	0.0344079628283896	0.029177952478474378	0.0
30	0.0019268459183898175	0.0	0.0344079628283896	0.05202483979652507	0.0
31	0.0019268459183898175	0.0	0.0344079628283896	0.11863865583228733	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCGTA	35	8.8576716E-4	26.428572	37
GTCGTTG	35	8.8576716E-4	26.428572	21
TAATACT	75	1.3671524E-8	24.666666	4
GTATCAA	850	0.0	23.288233	1
GGTATCA	300	0.0	22.816668	1
TCGCCGT	50	2.6971742E-4	22.2	25
CGTCCGC	50	2.6971742E-4	22.2	29
ACGTGGG	85	5.1561074E-8	21.764706	36
GCCGGCA	60	3.717262E-5	21.583332	15
CGTGGGA	90	9.433643E-8	20.555557	37
TCGTTGG	45	0.003820847	20.555557	22
TCACCGT	45	0.003820847	20.555557	22
GGAGTCG	45	0.003820847	20.555557	18
CCAACGA	45	0.003820847	20.555557	32
GTCGCCG	55	5.133793E-4	20.181818	24
CGAGTCG	55	5.133793E-4	20.181818	21
GAGTCGC	55	5.133793E-4	20.181818	22
GTCCTAT	55	5.133793E-4	20.181818	1
GTGTAGA	55	5.133793E-4	20.181818	1
GTCCGCC	55	5.133793E-4	20.181818	30
>>END_MODULE