FastQCFastQC Report
Fri 10 Feb 2017
ERR1632969.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632969.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388148
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13360.344198604655956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9370.2414027638941847No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6890.17750960973649227No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC6310.16256685594154807No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT6270.16153632119706915No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA6180.1592176180219916No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG5810.1496851716355617No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT5470.14092562630749095No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA5000.12881684305986377No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC4790.12340653565134949No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC4750.12237600090687058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4570.11773859455671547No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT4510.11619279243999711No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG4380.11284355452044065No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA4360.1123282871482012No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA4250.1094943166008842No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA4050.10434164287848964No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC3980.10253820707565155No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAA250.00549191529.59999816
AGGCGAC250.00549191529.59999814
GTACGAT250.00549191529.59999820
TTCGCCG556.24379E-726.90909424
GTGCCAA358.8587706E-426.4285720
GCCGGCA509.070847E-625.89999815
GTATAGA509.070847E-625.8999981
GGTATCA2300.025.739131
GTATCAA7300.024.3287681
CCGGCAG551.8984512E-523.54545616
ACGGGTA551.8984512E-523.54545616
CCGTTAA400.001928895423.12500216
GCCGCTC603.7181053E-521.58333427
TCGCCGC705.087568E-621.14285725
GTCGCCA909.4378265E-820.55555512
TATAGGT450.003821311820.5555555
CGGAGTT555.134661E-420.1818224
CCGCTCT656.887085E-519.92307728
CGCTCTC656.887085E-519.92307729
GCTTCGC759.239866E-619.73333222
Produced by FastQC (version 0.11.5)