##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632969.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	388148
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.778308789430834	33.0	33.0	33.0	33.0	33.0
2	31.268521285695147	33.0	33.0	33.0	27.0	33.0
3	31.71354740975092	33.0	33.0	33.0	27.0	33.0
4	31.828511289508125	33.0	33.0	33.0	27.0	33.0
5	31.93478003235879	33.0	33.0	33.0	33.0	33.0
6	34.846246792460605	37.0	37.0	37.0	27.0	37.0
7	35.006121376382204	37.0	37.0	37.0	33.0	37.0
8	35.177391098240875	37.0	37.0	37.0	33.0	37.0
9	35.27478178426786	37.0	37.0	37.0	33.0	37.0
10	35.29381318466152	37.0	37.0	37.0	33.0	37.0
11	35.320156229067265	37.0	37.0	37.0	33.0	37.0
12	35.29349114255387	37.0	37.0	37.0	33.0	37.0
13	35.3213954470975	37.0	37.0	37.0	33.0	37.0
14	35.282727207147786	37.0	37.0	37.0	33.0	37.0
15	35.292128260354296	37.0	37.0	37.0	33.0	37.0
16	35.301310324927606	37.0	37.0	37.0	33.0	37.0
17	35.26650143759597	37.0	37.0	37.0	33.0	37.0
18	35.26238445129177	37.0	37.0	37.0	33.0	37.0
19	35.285489040262995	37.0	37.0	37.0	33.0	37.0
20	35.293437039479784	37.0	37.0	37.0	33.0	37.0
21	35.27723960963344	37.0	37.0	37.0	33.0	37.0
22	35.28052443913147	37.0	37.0	37.0	33.0	37.0
23	35.25953244638643	37.0	37.0	37.0	33.0	37.0
24	35.248760781969764	37.0	37.0	37.0	33.0	37.0
25	35.250249904675535	37.0	37.0	37.0	33.0	37.0
26	35.20782278924534	37.0	37.0	37.0	33.0	37.0
27	35.175685563238765	37.0	37.0	37.0	33.0	37.0
28	35.139220091305376	37.0	37.0	37.0	33.0	37.0
29	35.174567433041005	37.0	37.0	37.0	33.0	37.0
30	35.13827715201418	37.0	37.0	37.0	33.0	37.0
31	35.12812896111792	37.0	37.0	37.0	33.0	37.0
32	35.156252254294756	37.0	37.0	37.0	33.0	37.0
33	35.14218803136948	37.0	37.0	37.0	33.0	37.0
34	35.06329029133217	37.0	37.0	37.0	33.0	37.0
35	35.0656656739182	37.0	37.0	37.0	27.0	37.0
36	35.046860991168316	37.0	37.0	37.0	27.0	37.0
37	35.07904459123839	37.0	37.0	37.0	33.0	37.0
38	34.938389480301325	37.0	37.0	37.0	27.0	37.0
39	35.009934354936775	37.0	37.0	37.0	27.0	37.0
40	35.032678256747424	37.0	37.0	37.0	27.0	37.0
41	35.056875212547794	37.0	37.0	37.0	27.0	37.0
42	34.972618691839195	37.0	37.0	37.0	27.0	37.0
43	34.760941702649504	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	11.0
16	23.0
17	25.0
18	14.0
19	21.0
20	35.0
21	77.0
22	216.0
23	495.0
24	1020.0
25	1848.0
26	3103.0
27	4731.0
28	6766.0
29	8811.0
30	11665.0
31	15030.0
32	18943.0
33	24783.0
34	35688.0
35	65308.0
36	189532.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.41448107422942	21.096849655286128	13.998268701629277	24.49040056885518
2	15.277677586899843	23.842967115636306	38.9907973247318	21.888557972732052
3	17.54743036161464	27.2919092717211	31.62324680276595	23.53741356389831
4	12.693354081432856	18.395818090006905	39.263116130960356	29.64771169759988
5	13.116388594041448	38.081350412729165	35.828086193926026	12.97417479930336
6	28.71662355596319	40.166637468182245	18.01580840298031	13.100930572874264
7	24.99098282098581	33.29039438564671	24.324484474994073	17.394138318373404
8	23.309407751682347	37.1546420437565	21.224893597287632	18.311056607273514
9	25.19786267093995	16.322382184115337	20.436019250389027	38.04373589455569
10	14.125539742572421	29.19633747951812	34.64554757463648	22.032575203272977
11	32.50718797984274	24.463606665498727	24.996908395766564	18.03229695889197
12	21.973834722837683	26.205725651040325	31.040221771077015	20.780217855044985
13	27.106413017714893	22.736945701124313	27.519915083937057	22.636726197223737
14	21.404721910199203	21.8578995640838	28.168894339272647	28.568484186444348
15	23.20377794037326	29.43696734235395	25.290353164256935	22.06890155301586
16	22.432937951503035	29.467368117316074	25.26278635984212	22.83690757133877
17	21.96636334594021	28.38144213032142	26.71532508218515	22.936869441553224
18	21.881601863206818	28.0990756103342	28.16863670558653	21.85068582087245
19	23.848119789358698	27.27799705267063	28.423436421158936	20.45044673681173
20	23.70564836093449	26.64576398693282	28.55534486845224	21.093242783680452
21	21.905046528643712	28.29075507280728	27.41093603470841	22.393262363840595
22	21.575790677782702	28.226089017591228	27.593598318167295	22.604521986458774
23	21.69971248080629	28.145192040149634	27.99447633376959	22.16061914527448
24	22.9940641198718	28.077176747014025	27.129084782093425	21.799674351020744
25	22.6923750734256	27.395478013541226	28.027195811906797	21.884951101126372
26	22.476478044457266	27.531508599812444	27.647186124880204	22.34482723085009
27	22.21266114987067	27.390067706132715	27.605191834042685	22.792079309953937
28	21.504683780413654	27.942949596545645	28.24335047456125	22.309016148479447
29	22.70371095561487	28.061461092160723	27.379247091315683	21.855580860908724
30	22.05060956130136	28.15266341704711	27.673207127178294	22.123519894473244
31	22.61714603707864	27.65929490812783	27.95196677555984	21.771592279233694
32	21.78653503302864	27.614466646742997	27.995249234827952	22.603749085400416
33	21.90968393499387	27.411451302080646	28.373713119737832	22.305151643187653
34	21.858157197769923	27.54464791780455	28.03415192143203	22.5630429629935
35	22.27629667034224	27.976957243113453	27.548512423096344	22.198233663447965
36	22.37316693632326	27.988808392674958	27.336479899419807	22.301544771581973
37	22.92785226253903	27.14634623906345	27.301956985479762	22.623844512917753
38	22.94640188793965	27.602873130867607	27.5139895091563	21.93673547203644
39	22.233014211074124	27.453960860290405	28.048837041540853	22.264187887094614
40	22.228376804723972	27.425105887444996	28.069705370116555	22.27681193771448
41	21.94729845316735	27.53460020404588	28.18229129095087	22.335810051835896
42	22.490132629821613	27.260220328328373	27.84736749899523	22.402279542854785
43	22.271916897678203	27.19065923307604	27.82701443779177	22.710409431453982
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	16.0
2	27.0
3	76.5
4	126.0
5	126.0
6	170.0
7	214.0
8	209.0
9	204.0
10	298.5
11	393.0
12	393.0
13	569.5
14	746.0
15	1466.0
16	2186.0
17	2498.0
18	2810.0
19	2810.0
20	2760.0
21	2710.0
22	3420.5
23	4131.0
24	5432.5
25	6734.0
26	6734.0
27	8408.0
28	10082.0
29	13131.0
30	16180.0
31	17305.5
32	18431.0
33	18431.0
34	20204.0
35	21977.0
36	23649.5
37	25322.0
38	27006.0
39	28690.0
40	28690.0
41	30609.0
42	32528.0
43	30620.0
44	28712.0
45	28902.0
46	29092.0
47	29092.0
48	28532.5
49	27973.0
50	28318.5
51	28664.0
52	28690.5
53	28717.0
54	28717.0
55	24342.0
56	19967.0
57	17610.0
58	15253.0
59	13374.0
60	11495.0
61	11495.0
62	10024.5
63	8554.0
64	7018.5
65	5483.0
66	4666.0
67	3849.0
68	3849.0
69	3202.0
70	2555.0
71	2138.0
72	1721.0
73	1461.5
74	1202.0
75	1202.0
76	894.5
77	587.0
78	475.5
79	364.0
80	277.0
81	190.0
82	190.0
83	158.5
84	127.0
85	99.5
86	72.0
87	60.0
88	48.0
89	48.0
90	35.5
91	23.0
92	13.5
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	388148.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.95187250082688
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.17601161603004	55.865727742693764
2	5.320893633384391	6.592786479317825
3	1.4251278634742226	2.6486801908599253
4	0.7113638445096151	1.7628128878703087
5	0.4132697131743689	1.2801416289515897
6	0.28082567985119666	1.0438606027859616
7	0.20095070498889123	0.8714490718096144
8	0.1507604663428723	0.747191455123106
9	0.1295783856632406	0.7224861264724848
>10	0.9697586558775225	12.15853786380141
>50	0.13505670192918712	5.9255100387414315
>100	0.083047288763807	8.830579422750509
>500	0.00293601525932651	1.2030832958843825
>1k	4.194307513323586E-4	0.34715319293769653
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1336	0.344198604655956	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	937	0.2414027638941847	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	689	0.17750960973649227	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	631	0.16256685594154807	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	627	0.16153632119706915	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	618	0.1592176180219916	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	581	0.1496851716355617	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	547	0.14092562630749095	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	500	0.12881684305986377	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	479	0.12340653565134949	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	475	0.12237600090687058	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	457	0.11773859455671547	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	451	0.11619279243999711	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	438	0.11284355452044065	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	436	0.1123282871482012	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	425	0.1094943166008842	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	405	0.10434164287848964	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	398	0.10253820707565155	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.152673722394551E-4	0.0	0.0
2	0.0	0.0	5.152673722394551E-4	0.0	0.0
3	0.0	0.0	5.152673722394551E-4	0.0	0.0
4	0.0	0.0	5.152673722394551E-4	0.0	0.0
5	0.0	2.5763368611972755E-4	5.152673722394551E-4	0.0	0.0
6	0.0	2.5763368611972755E-4	0.0015458021167183652	0.0	0.0
7	0.0	2.5763368611972755E-4	0.004379772664035368	0.0	0.0
8	0.0	2.5763368611972755E-4	0.0054103074085142784	0.0	0.0
9	0.0	2.5763368611972755E-4	0.006698475839112916	7.729010583591826E-4	0.0
10	2.5763368611972755E-4	2.5763368611972755E-4	0.007729010583591826	7.729010583591826E-4	0.0
11	2.5763368611972755E-4	2.5763368611972755E-4	0.011593515875387738	7.729010583591826E-4	0.0
12	2.5763368611972755E-4	2.5763368611972755E-4	0.012108783247627194	7.729010583591826E-4	0.0
13	2.5763368611972755E-4	2.5763368611972755E-4	0.012624050619866649	7.729010583591826E-4	0.0
14	2.5763368611972755E-4	2.5763368611972755E-4	0.013396951678225832	7.729010583591826E-4	0.0
15	2.5763368611972755E-4	2.5763368611972755E-4	0.01468512010882447	7.729010583591826E-4	0.0
16	2.5763368611972755E-4	2.5763368611972755E-4	0.015973288539423106	7.729010583591826E-4	0.0
17	2.5763368611972755E-4	2.5763368611972755E-4	0.016230922225542833	7.729010583591826E-4	0.0
18	2.5763368611972755E-4	2.5763368611972755E-4	0.017003823283902018	7.729010583591826E-4	0.0
19	2.5763368611972755E-4	2.5763368611972755E-4	0.017003823283902018	7.729010583591826E-4	0.0
20	2.5763368611972755E-4	2.5763368611972755E-4	0.017519090656141472	7.729010583591826E-4	0.0
21	5.152673722394551E-4	2.5763368611972755E-4	0.017519090656141472	7.729010583591826E-4	0.0
22	5.152673722394551E-4	2.5763368611972755E-4	0.017519090656141472	0.0012881684305986376	0.0
23	5.152673722394551E-4	2.5763368611972755E-4	0.0177767243422612	0.0012881684305986376	0.0
24	5.152673722394551E-4	2.5763368611972755E-4	0.0177767243422612	0.0015458021167183652	0.0
25	5.152673722394551E-4	2.5763368611972755E-4	0.0177767243422612	0.0018034358028380926	0.0
26	5.152673722394551E-4	2.5763368611972755E-4	0.0177767243422612	0.0018034358028380926	0.0
27	5.152673722394551E-4	2.5763368611972755E-4	0.0177767243422612	0.002576336861197275	0.0
28	5.152673722394551E-4	2.5763368611972755E-4	0.0177767243422612	0.008759545328070736	0.0
29	5.152673722394551E-4	2.5763368611972755E-4	0.0177767243422612	0.022156497006296568	0.0
30	5.152673722394551E-4	2.5763368611972755E-4	0.0177767243422612	0.04096375609303668	0.0
31	5.152673722394551E-4	2.5763368611972755E-4	0.0177767243422612	0.08939888908354546	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACAA	25	0.005491915	29.599998	16
AGGCGAC	25	0.005491915	29.599998	14
GTACGAT	25	0.005491915	29.599998	20
TTCGCCG	55	6.24379E-7	26.909094	24
GTGCCAA	35	8.8587706E-4	26.42857	20
GCCGGCA	50	9.070847E-6	25.899998	15
GTATAGA	50	9.070847E-6	25.899998	1
GGTATCA	230	0.0	25.73913	1
GTATCAA	730	0.0	24.328768	1
CCGGCAG	55	1.8984512E-5	23.545456	16
ACGGGTA	55	1.8984512E-5	23.545456	16
CCGTTAA	40	0.0019288954	23.125002	16
GCCGCTC	60	3.7181053E-5	21.583334	27
TCGCCGC	70	5.087568E-6	21.142857	25
GTCGCCA	90	9.4378265E-8	20.555555	12
TATAGGT	45	0.0038213118	20.555555	5
CGGAGTT	55	5.134661E-4	20.18182	24
CCGCTCT	65	6.887085E-5	19.923077	28
CGCTCTC	65	6.887085E-5	19.923077	29
GCTTCGC	75	9.239866E-6	19.733332	22
>>END_MODULE