FastQCFastQC Report
Fri 10 Feb 2017
ERR1632968.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632968.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347170
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8620.24829334331883515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5820.16764121323847106No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4010.11550537200795No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG3910.11262493879079415No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3880.11176080882564737No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGACG200.001840166737.029
GCGCGAT303.59435E-430.83333233
CGCGCGA303.59435E-430.83333232
CGCGATA303.59435E-430.83333234
TCGGCGT250.00549112729.59999811
AATCTCG250.00549112729.59999836
GACGCAC250.00549112729.59999832
TAGGAAC250.00549112729.59999818
GGTATCA1300.027.0384621
GCGCGCG358.856877E-426.42857231
GCGATAC358.856877E-426.42857235
CGCACTC358.856877E-426.42857234
GTATCAA5550.026.3333341
GTAAATC551.8977033E-523.54545416
CGGCGTA400.001928487223.12500212
ACGCCGG400.001928487223.12500235
GTTAGCC400.001928487223.12500228
AGGCGCG400.001928487223.12500229
GACTGAC400.001928487223.1250027
CGATACA400.001928487223.12500236
Produced by FastQC (version 0.11.5)