##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632968.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	347170
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.733925742431662	33.0	33.0	33.0	27.0	33.0
2	31.254448829103897	33.0	33.0	33.0	27.0	33.0
3	31.686634789872397	33.0	33.0	33.0	27.0	33.0
4	31.81026010311951	33.0	33.0	33.0	27.0	33.0
5	31.909937494599188	33.0	33.0	33.0	33.0	33.0
6	34.82665552899156	37.0	37.0	37.0	27.0	37.0
7	34.987277126479825	37.0	37.0	37.0	33.0	37.0
8	35.16167871647896	37.0	37.0	37.0	33.0	37.0
9	35.24247486822018	37.0	37.0	37.0	33.0	37.0
10	35.27027680963217	37.0	37.0	37.0	33.0	37.0
11	35.308733473514415	37.0	37.0	37.0	33.0	37.0
12	35.24659676815393	37.0	37.0	37.0	33.0	37.0
13	35.29128956995132	37.0	37.0	37.0	33.0	37.0
14	35.26935795143589	37.0	37.0	37.0	33.0	37.0
15	35.28531843189216	37.0	37.0	37.0	33.0	37.0
16	35.28717055045079	37.0	37.0	37.0	33.0	37.0
17	35.25992453264971	37.0	37.0	37.0	33.0	37.0
18	35.264216378143274	37.0	37.0	37.0	33.0	37.0
19	35.270939309272116	37.0	37.0	37.0	33.0	37.0
20	35.26331768297952	37.0	37.0	37.0	33.0	37.0
21	35.281830227266184	37.0	37.0	37.0	33.0	37.0
22	35.27339055793991	37.0	37.0	37.0	33.0	37.0
23	35.24935334274275	37.0	37.0	37.0	33.0	37.0
24	35.251850678342024	37.0	37.0	37.0	33.0	37.0
25	35.25040470086701	37.0	37.0	37.0	33.0	37.0
26	35.213635970850014	37.0	37.0	37.0	33.0	37.0
27	35.16263502030706	37.0	37.0	37.0	33.0	37.0
28	35.14714117003197	37.0	37.0	37.0	33.0	37.0
29	35.1670420831293	37.0	37.0	37.0	33.0	37.0
30	35.12555520350261	37.0	37.0	37.0	33.0	37.0
31	35.12723737650143	37.0	37.0	37.0	33.0	37.0
32	35.16229800962065	37.0	37.0	37.0	33.0	37.0
33	35.15554627415963	37.0	37.0	37.0	33.0	37.0
34	35.083048650517036	37.0	37.0	37.0	33.0	37.0
35	35.078445718236026	37.0	37.0	37.0	33.0	37.0
36	35.06830659331163	37.0	37.0	37.0	33.0	37.0
37	35.088628049658666	37.0	37.0	37.0	33.0	37.0
38	34.964357519370914	37.0	37.0	37.0	27.0	37.0
39	35.02640493130167	37.0	37.0	37.0	27.0	37.0
40	35.083195552611116	37.0	37.0	37.0	33.0	37.0
41	35.096145980355445	37.0	37.0	37.0	33.0	37.0
42	35.01461243771063	37.0	37.0	37.0	27.0	37.0
43	34.812806406083475	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	8.0
16	12.0
17	19.0
18	14.0
19	8.0
20	30.0
21	60.0
22	163.0
23	432.0
24	860.0
25	1579.0
26	2801.0
27	4122.0
28	5886.0
29	7902.0
30	10606.0
31	13463.0
32	17023.0
33	22861.0
34	32353.0
35	59318.0
36	167648.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.78635826828355	20.456548664919204	13.637987153267852	25.119105913529395
2	16.113431460091597	22.620330097646686	37.86387072615721	23.402367716104504
3	17.679234956937524	26.968920125586887	29.66932626667051	25.68251865080508
4	12.736699599619783	17.884609845320735	39.22804389780223	30.15064665725725
5	13.851715297980816	36.93147449376386	35.78880663651813	13.42800357173719
6	29.763516432871505	39.81392401417173	17.363827519658955	13.058732033297806
7	27.066566811648475	31.490048103234724	24.107497767664256	17.335887317452546
8	24.35147046115736	36.28942592965982	20.620733358297088	18.738370250885733
9	25.755969697842556	15.732638188783593	20.11233689546908	38.399055217904774
10	14.97854077253219	28.030935852752254	34.05881844629432	22.931704928421233
11	34.09107929832647	23.665927355474263	24.104617334447102	18.138376011752165
12	22.98556902958205	25.882132672753983	29.848201169455884	21.28409712820808
13	27.916294610709453	21.931906558746437	25.932252210732493	24.21954661981162
14	22.12604775758274	20.589048592908373	27.25264279747674	30.032260852032145
15	23.818014229340093	28.67471267678659	24.06429126940692	23.4429818244664
16	23.538324163954258	27.97707175159144	25.030964657084425	23.453639427369875
17	22.61543336117752	27.685571910015266	26.152029265201488	23.546965463605726
18	23.156954806002823	27.291816689230057	27.296713425699227	22.25451507906789
19	24.628856179969468	26.334360687847454	26.99426793789786	22.04251519428522
20	23.999193478699198	26.47982256531382	27.082409194342826	22.43857476164415
21	23.04605812714232	27.03286574300775	27.056485295388427	22.864590834461502
22	23.162139585793703	26.668490941037533	26.991963591324136	23.177405881844628
23	22.85940605467062	26.871561482847024	27.48394158481436	22.785090877668
24	23.09272114526025	27.387447072039635	25.831725091453755	23.688106691246364
25	23.466025290203646	26.53714318633523	27.352305786790332	22.644525736670793
26	23.27908517441023	26.81625716507763	27.201371086211367	22.703286574300773
27	23.013221188466744	27.021344010139124	26.333496557882306	23.631938243511826
28	22.86862344096552	26.716594175764037	27.69622951291874	22.7185528703517
29	24.173747731658842	26.462828009332608	26.61376270991157	22.749661549096984
30	23.695883860932685	26.55644208889017	26.757784370769365	22.98988967940778
31	23.591324135149925	26.374686752887634	27.003197280871046	23.030791831091395
32	22.677650718668087	26.600800760434367	27.088170060777138	23.6333784601204
33	22.64423769334908	26.716882219085754	27.404153584699138	23.23472650286603
34	23.697900164184695	26.11631189330875	27.165077627675206	23.020710314831348
35	23.195264567790996	26.994844024541294	26.853702796900652	22.956188610767057
36	23.90413918253305	26.725235475415506	26.41155629806723	22.959069043984215
37	23.26612322493303	26.0543825791399	26.963447302474293	23.716046893452774
38	23.390845983235877	26.900365815018578	26.521876890284297	23.186911311461245
39	22.744476769306104	26.720626782268052	27.314860154967306	23.220036293458538
40	22.920471238874327	26.981305988420658	27.526860039749977	22.571362732955038
41	23.113172221102054	26.047757582740445	27.21202868911484	23.62704150704266
42	22.70299853097906	27.153267851484863	26.982170118385806	23.161563499150272
43	23.661894748970244	25.90690439842152	27.125327649278454	23.30587320332978
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	9.0
2	14.0
3	42.5
4	71.0
5	71.0
6	103.5
7	136.0
8	122.5
9	109.0
10	141.5
11	174.0
12	174.0
13	294.0
14	414.0
15	741.0
16	1068.0
17	1296.0
18	1524.0
19	1524.0
20	1461.5
21	1399.0
22	1591.5
23	1784.0
24	2570.5
25	3357.0
26	3357.0
27	4578.5
28	5800.0
29	7426.5
30	9053.0
31	11105.5
32	13158.0
33	13158.0
34	15681.0
35	18204.0
36	20309.5
37	22415.0
38	24470.0
39	26525.0
40	26525.0
41	28019.0
42	29513.0
43	30166.5
44	30820.0
45	29953.5
46	29087.0
47	29087.0
48	27858.5
49	26630.0
50	26004.0
51	25378.0
52	23821.0
53	22264.0
54	22264.0
55	21494.5
56	20725.0
57	17972.5
58	15220.0
59	14094.5
60	12969.0
61	12969.0
62	11560.0
63	10151.0
64	8441.0
65	6731.0
66	5670.0
67	4609.0
68	4609.0
69	3822.5
70	3036.0
71	2501.0
72	1966.0
73	1599.5
74	1233.0
75	1233.0
76	956.5
77	680.0
78	541.5
79	403.0
80	320.0
81	237.0
82	237.0
83	192.5
84	148.0
85	113.5
86	79.0
87	67.0
88	55.0
89	55.0
90	36.5
91	18.0
92	12.5
93	7.0
94	4.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	347170.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.937528547968505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.08842818880763	56.32156392067561
2	6.415517549473039	8.203846729388507
3	1.8009879788500054	3.4545216093681086
4	0.8927592817062001	2.283232882422161
5	0.531796922164958	1.7000890446321895
6	0.37881559476430504	1.4532319742795052
7	0.27819621477145623	1.2451024896720733
8	0.1947725216265899	0.9962618929487922
9	0.17896097303856515	1.0298090090362932
>10	1.0886441336530506	13.547053848667865
>50	0.10710051444454408	4.675615161337935
>100	0.04311249522131352	4.6706824656849415
>500	9.076314783434425E-4	0.4189889718860071
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	862	0.24829334331883515	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	582	0.16764121323847106	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	401	0.11550537200795	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	391	0.11262493879079415	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	388	0.11176080882564737	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	8.64129965146758E-4	0.0	0.0
2	2.88043321715586E-4	0.0	8.64129965146758E-4	0.0	0.0
3	2.88043321715586E-4	0.0	0.001152173286862344	0.0	0.0
4	2.88043321715586E-4	0.0	0.00144021660857793	0.0	0.0
5	2.88043321715586E-4	0.0	0.001728259930293516	0.0	0.0
6	2.88043321715586E-4	0.0	0.002016303252009102	0.0	0.0
7	2.88043321715586E-4	0.0	0.003456519860587032	0.0	0.0
8	2.88043321715586E-4	0.0	0.004608693147449376	0.0	0.0
9	2.88043321715586E-4	0.0	0.005184779790880548	0.0	0.0
10	2.88043321715586E-4	0.0	0.005472823112596135	0.0	0.0
11	2.88043321715586E-4	0.0	0.00576086643431172	0.0	0.0
12	2.88043321715586E-4	0.0	0.007201083042889651	2.88043321715586E-4	0.0
13	2.88043321715586E-4	0.0	0.007777169686320823	2.88043321715586E-4	0.0
14	2.88043321715586E-4	0.0	0.008929342973183168	2.88043321715586E-4	0.0
15	2.88043321715586E-4	0.0	0.009793472938329925	2.88043321715586E-4	0.0
16	2.88043321715586E-4	0.0	0.010369559581761096	2.88043321715586E-4	0.0
17	2.88043321715586E-4	0.0	0.01094564622519227	2.88043321715586E-4	0.0
18	2.88043321715586E-4	0.0	0.01094564622519227	2.88043321715586E-4	0.0
19	2.88043321715586E-4	0.0	0.011233689546907856	2.88043321715586E-4	0.0
20	2.88043321715586E-4	0.0	0.01152173286862344	2.88043321715586E-4	0.0
21	2.88043321715586E-4	0.0	0.011809776190339027	5.76086643431172E-4	0.0
22	2.88043321715586E-4	0.0	0.012097819512054613	8.64129965146758E-4	0.0
23	2.88043321715586E-4	0.0	0.012097819512054613	8.64129965146758E-4	0.0
24	2.88043321715586E-4	0.0	0.0123858628337702	8.64129965146758E-4	0.0
25	2.88043321715586E-4	0.0	0.0123858628337702	8.64129965146758E-4	0.0
26	2.88043321715586E-4	0.0	0.0123858628337702	0.001152173286862344	0.0
27	2.88043321715586E-4	0.0	0.0123858628337702	0.002592389895440274	0.0
28	2.88043321715586E-4	0.0	0.0123858628337702	0.007201083042889651	0.0
29	2.88043321715586E-4	2.88043321715586E-4	0.0123858628337702	0.018146729268081918	0.0
30	2.88043321715586E-4	2.88043321715586E-4	0.0123858628337702	0.033701068640723565	0.0
31	2.88043321715586E-4	2.88043321715586E-4	0.0123858628337702	0.08900538641011609	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGACG	20	0.0018401667	37.0	29
GCGCGAT	30	3.59435E-4	30.833332	33
CGCGCGA	30	3.59435E-4	30.833332	32
CGCGATA	30	3.59435E-4	30.833332	34
TCGGCGT	25	0.005491127	29.599998	11
AATCTCG	25	0.005491127	29.599998	36
GACGCAC	25	0.005491127	29.599998	32
TAGGAAC	25	0.005491127	29.599998	18
GGTATCA	130	0.0	27.038462	1
GCGCGCG	35	8.856877E-4	26.428572	31
GCGATAC	35	8.856877E-4	26.428572	35
CGCACTC	35	8.856877E-4	26.428572	34
GTATCAA	555	0.0	26.333334	1
GTAAATC	55	1.8977033E-5	23.545454	16
CGGCGTA	40	0.0019284872	23.125002	12
ACGCCGG	40	0.0019284872	23.125002	35
GTTAGCC	40	0.0019284872	23.125002	28
AGGCGCG	40	0.0019284872	23.125002	29
GACTGAC	40	0.0019284872	23.125002	7
CGATACA	40	0.0019284872	23.125002	36
>>END_MODULE