FastQCFastQC Report
Fri 10 Feb 2017
ERR1632967.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632967.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339773
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14650.4311702224720622No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10140.29843454306257416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5370.1580466958822508No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4760.1400935330352912No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4250.12508351163865286No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4130.12155174189826738No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4100.120668799463171No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC3830.11272231754730364No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG3630.10683603464666114No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACG200.001840111637.032
GGTATCA2500.026.641
ACGGGAC809.731593E-1025.43750216
CTCTAAT701.9146319E-723.7857131
GTATCAA7800.023.7179491
TAGGACC903.805326E-922.611114
TCTATCG502.6966768E-422.231
ACTCAGC806.930459E-720.81258
TATTAGA1251.8189894E-1120.722
CGGGACT450.003820346720.55555517
CTACACT450.003820346720.5555554
CCGTTTA450.003820346720.55555527
TAATACT1450.020.4137944
GGACCGT555.132853E-420.1818186
TCTATAC656.883855E-519.9230773
TAGACTG500.007024934518.55
GACCGTT500.007024934518.57
ATACTGG1205.135007E-918.56
GTACTTA500.007024934518.53
TTAACGG1103.8278813E-818.535