##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632967.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	339773
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.730425901999276	33.0	33.0	33.0	27.0	33.0
2	31.24224702963449	33.0	33.0	33.0	27.0	33.0
3	31.687058712728792	33.0	33.0	33.0	27.0	33.0
4	31.823652850579652	33.0	33.0	33.0	27.0	33.0
5	31.93384700962113	33.0	33.0	33.0	33.0	33.0
6	34.805340624475754	37.0	37.0	37.0	27.0	37.0
7	34.976396005568425	37.0	37.0	37.0	27.0	37.0
8	35.14288068798874	37.0	37.0	37.0	33.0	37.0
9	35.228352458847525	37.0	37.0	37.0	33.0	37.0
10	35.26003537656023	37.0	37.0	37.0	33.0	37.0
11	35.28165275051284	37.0	37.0	37.0	33.0	37.0
12	35.23359419377055	37.0	37.0	37.0	33.0	37.0
13	35.260211965047255	37.0	37.0	37.0	33.0	37.0
14	35.2309012193435	37.0	37.0	37.0	33.0	37.0
15	35.24999926421464	37.0	37.0	37.0	33.0	37.0
16	35.268470419956856	37.0	37.0	37.0	33.0	37.0
17	35.217904306698884	37.0	37.0	37.0	33.0	37.0
18	35.23124851003464	37.0	37.0	37.0	33.0	37.0
19	35.222881158891376	37.0	37.0	37.0	33.0	37.0
20	35.22193346734437	37.0	37.0	37.0	33.0	37.0
21	35.22857613759775	37.0	37.0	37.0	33.0	37.0
22	35.20878056820289	37.0	37.0	37.0	33.0	37.0
23	35.19734940680984	37.0	37.0	37.0	33.0	37.0
24	35.214234209310334	37.0	37.0	37.0	33.0	37.0
25	35.22461761234707	37.0	37.0	37.0	33.0	37.0
26	35.17889590991633	37.0	37.0	37.0	33.0	37.0
27	35.10531443051684	37.0	37.0	37.0	33.0	37.0
28	35.08439163794651	37.0	37.0	37.0	33.0	37.0
29	35.11663375253478	37.0	37.0	37.0	33.0	37.0
30	35.072186430352026	37.0	37.0	37.0	33.0	37.0
31	35.07379044244245	37.0	37.0	37.0	33.0	37.0
32	35.11588325146495	37.0	37.0	37.0	33.0	37.0
33	35.08578374385252	37.0	37.0	37.0	33.0	37.0
34	35.02924893973329	37.0	37.0	37.0	27.0	37.0
35	35.0270504130699	37.0	37.0	37.0	27.0	37.0
36	34.998301807383164	37.0	37.0	37.0	27.0	37.0
37	35.02914887292398	37.0	37.0	37.0	27.0	37.0
38	34.85956506255647	37.0	37.0	37.0	27.0	37.0
39	34.94911897060685	37.0	37.0	37.0	27.0	37.0
40	34.973488181815505	37.0	37.0	37.0	27.0	37.0
41	35.00337284010207	37.0	37.0	37.0	27.0	37.0
42	34.90499833712508	37.0	37.0	37.0	27.0	37.0
43	34.69611181582998	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	10.0
16	20.0
17	21.0
18	24.0
19	16.0
20	31.0
21	69.0
22	172.0
23	442.0
24	857.0
25	1668.0
26	2850.0
27	4303.0
28	6092.0
29	8119.0
30	10631.0
31	13616.0
32	16967.0
33	22112.0
34	31658.0
35	57483.0
36	162612.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.27844178319054	20.507809625838426	13.656470643635604	24.557277947335425
2	16.027171081869366	22.839071968637885	38.11603629482037	23.017720654672384
3	17.904012384739225	27.08161036927596	30.011507683070754	25.002869562914064
4	12.578692244527964	18.14211252807021	39.38129280431347	29.897902423088357
5	13.981099145606038	36.93407068837136	35.087838056584836	13.996992109437773
6	29.349300856748478	39.95226224567579	17.351584734513924	13.34685216306181
7	26.733436735702952	32.25359283992548	23.243165289766992	17.769805134604574
8	24.277385195409877	35.83156990108102	20.579622277226267	19.31142262628284
9	25.94555776945196	15.426770226003834	20.26823791178228	38.35943409276194
10	15.19779382116884	27.982211653074256	33.570648638944235	23.249345886812666
11	34.19518325470241	23.826790239365696	23.810308647243893	18.167717858688007
12	23.34146621420772	25.681557981358143	30.098918984145296	20.87805682028884
13	28.28564953660238	22.64423600462662	25.687149950113753	23.38296450865725
14	21.989681346075173	21.083488093521265	27.71497440938509	29.211856151018473
15	24.036047596483535	28.527575763818785	24.660582212241703	22.775794427455978
16	22.811112124859832	28.39778322585962	25.300715477686573	23.490389171593975
17	22.388771326738734	27.259964741165426	26.392620955755753	23.958642976340087
18	23.129265715639562	26.873235954593216	27.8094492499404	22.188049079826826
19	24.956367927998986	26.300794942505735	27.111924726214266	21.630912403281013
20	24.462803106780115	26.0971295541435	27.40888769855168	22.031179640524705
21	23.146924564341486	26.890600489150106	27.50277391081693	22.459701035691477
22	22.88822243085825	26.903844625676555	27.259376112875362	22.948556830589837
23	22.96297822369641	26.773463459427322	27.92864647867841	22.334911838197854
24	23.040088529694824	27.149891250923407	26.43470787849535	23.375312340886413
25	23.283486327636393	26.88353694966934	27.630506249760874	22.2024704729334
26	23.429760457717357	27.04452678700191	27.206105252624546	22.319607502656186
27	23.14221553802097	26.942399778675764	26.853811221021097	23.06157346228217
28	22.63923266416107	26.84498179667013	27.69996438798845	22.815821151180344
29	23.9715927987215	26.76021932290088	26.729904965962568	22.538282912415053
30	23.465372469266246	26.54860745262278	27.629623307325772	22.3563967707852
31	23.414161808030656	26.352299917886352	27.34914192711016	22.88439634697283
32	22.186577509101664	26.52535663516524	27.778546264712027	23.509519591021064
33	22.383767986273188	26.721075541611604	28.197060978947707	22.698095493167497
34	22.78992150641752	26.377905248504145	27.722037948865864	23.110135296212473
35	22.842309424233235	27.269677107951484	27.327068366232748	22.560945101582526
36	23.678750224414536	26.663978597475374	27.16931598449553	22.48795519361456
37	23.42034240507633	26.314333393177208	27.010386346178183	23.254937855568276
38	23.22226898546971	26.755510296580365	27.02686793829998	22.995352779649945
39	22.88233614795761	26.546841567752587	27.644927642867444	22.92589464142236
40	22.706336289228396	27.392994734719945	27.555750456922713	22.34491851912895
41	23.015954769802192	26.111256633105047	27.440967940360185	23.431820656732583
42	22.546523708475952	27.191683859517973	27.523081586824144	22.73871084518193
43	23.28201475691123	26.040326924152303	27.404767300521232	23.272891018415237
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	14.0
2	20.0
3	73.0
4	126.0
5	126.0
6	161.5
7	197.0
8	197.0
9	197.0
10	235.0
11	273.0
12	273.0
13	481.5
14	690.0
15	1253.5
16	1817.0
17	2158.5
18	2500.0
19	2500.0
20	2243.0
21	1986.0
22	2203.5
23	2421.0
24	3318.0
25	4215.0
26	4215.0
27	5377.5
28	6540.0
29	7905.0
30	9270.0
31	11168.0
32	13066.0
33	13066.0
34	15352.0
35	17638.0
36	19050.0
37	20462.0
38	22123.5
39	23785.0
40	23785.0
41	25079.5
42	26374.0
43	27247.0
44	28120.0
45	27665.5
46	27211.0
47	27211.0
48	26776.5
49	26342.0
50	25850.0
51	25358.0
52	24472.5
53	23587.0
54	23587.0
55	22638.0
56	21689.0
57	18998.0
58	16307.0
59	14665.5
60	13024.0
61	13024.0
62	11268.5
63	9513.0
64	7800.0
65	6087.0
66	5093.5
67	4100.0
68	4100.0
69	3471.5
70	2843.0
71	2281.0
72	1719.0
73	1324.0
74	929.0
75	929.0
76	745.0
77	561.0
78	451.0
79	341.0
80	263.0
81	185.0
82	185.0
83	152.0
84	119.0
85	97.5
86	76.0
87	61.5
88	47.0
89	47.0
90	32.5
91	18.0
92	14.0
93	10.0
94	6.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	339773.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.42692281701527
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.48131633232435	57.00578938090574
2	6.345309063211819	8.176174745313105
3	1.839474033750018	3.5553495478894837
4	0.8655453075109811	2.230576828865589
5	0.4969139263152308	1.6007317588705692
6	0.34584288558302	1.3368955737762642
7	0.24748671383753523	1.1161365187453338
8	0.1789732501038377	0.9224556616600256
9	0.14369839523301478	0.8332240876745759
>10	0.8980904119950666	11.31370572973386
>50	0.09707831142837595	4.264887467058688
>100	0.058891108354650556	6.750070711694139
>500	4.6008678402070747E-4	0.1591774096337499
>1k	9.201735680414149E-4	0.7348245781788938
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1465	0.4311702224720622	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1014	0.29843454306257416	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	537	0.1580466958822508	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	476	0.1400935330352912	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	425	0.12508351163865286	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	413	0.12155174189826738	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	410	0.120668799463171	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	383	0.11272231754730364	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	363	0.10683603464666114	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.943141450321244E-4	0.0	0.0
2	0.0	0.0	2.943141450321244E-4	0.0	0.0
3	0.0	0.0	2.943141450321244E-4	0.0	0.0
4	0.0	0.0	2.943141450321244E-4	2.943141450321244E-4	0.0
5	0.0	0.0	5.886282900642488E-4	5.886282900642488E-4	0.0
6	0.0	0.0	8.829424350963732E-4	5.886282900642488E-4	0.0
7	0.0	0.0	0.0017658848701927463	5.886282900642488E-4	0.0
8	0.0	0.0	0.002060199015224871	5.886282900642488E-4	0.0
9	0.0	0.0	0.002943141450321244	8.829424350963732E-4	0.0
10	0.0	0.0	0.002943141450321244	8.829424350963732E-4	0.0
11	0.0	0.0	0.005297654610578239	8.829424350963732E-4	0.0
12	0.0	0.0	0.0055919687556103635	8.829424350963732E-4	0.0
13	0.0	0.0	0.0055919687556103635	8.829424350963732E-4	0.0
14	0.0	0.0	0.005886282900642488	8.829424350963732E-4	0.0
15	0.0	0.0	0.005886282900642488	8.829424350963732E-4	0.0
16	0.0	0.0	0.006474911190706737	8.829424350963732E-4	0.0
17	0.0	0.0	0.006474911190706737	0.001471570725160622	0.0
18	0.0	0.0	0.006474911190706737	0.001471570725160622	0.0
19	0.0	0.0	0.0067692253357388606	0.001471570725160622	0.0
20	0.0	0.0	0.0067692253357388606	0.001471570725160622	0.0
21	0.0	0.0	0.0067692253357388606	0.001471570725160622	0.0
22	0.0	0.0	0.00735785362580311	0.0017658848701927463	0.0
23	0.0	0.0	0.00735785362580311	0.002354513160256995	0.0
24	0.0	0.0	0.007652167770835234	0.002943141450321244	0.0
25	0.0	0.0	0.007652167770835234	0.002943141450321244	0.0
26	0.0	0.0	0.007652167770835234	0.0032374555953533684	0.0
27	0.0	0.0	0.007652167770835234	0.0044147121754818655	0.0
28	0.0	0.0	0.007652167770835234	0.00941805264102798	0.0
29	0.0	0.0	0.007652167770835234	0.02001336186218446	0.0
30	0.0	0.0	0.007652167770835234	0.037966524709144044	0.0
31	0.0	0.0	0.007652167770835234	0.09359189812021555	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAACG	20	0.0018401116	37.0	32
GGTATCA	250	0.0	26.64	1
ACGGGAC	80	9.731593E-10	25.437502	16
CTCTAAT	70	1.9146319E-7	23.785713	1
GTATCAA	780	0.0	23.717949	1
TAGGACC	90	3.805326E-9	22.61111	4
TCTATCG	50	2.6966768E-4	22.2	31
ACTCAGC	80	6.930459E-7	20.8125	8
TATTAGA	125	1.8189894E-11	20.72	2
CGGGACT	45	0.0038203467	20.555555	17
CTACACT	45	0.0038203467	20.555555	4
CCGTTTA	45	0.0038203467	20.555555	27
TAATACT	145	0.0	20.413794	4
GGACCGT	55	5.132853E-4	20.181818	6
TCTATAC	65	6.883855E-5	19.923077	3
TAGACTG	50	0.0070249345	18.5	5
GACCGTT	50	0.0070249345	18.5	7
ATACTGG	120	5.135007E-9	18.5	6
GTACTTA	50	0.0070249345	18.5	3
TTAACGG	110	3.8278813E-8	18.5	35
>>END_MODULE