FastQCFastQC Report
Fri 10 Feb 2017
ERR1632966.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632966.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386779
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10290.2660433994606739No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6890.17813790304023744No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4570.1181553290121749No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4300.11117459841408144No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG250.00549189129.65
GGTATCA2450.028.6938761
TATCGCG405.9300968E-527.7500027
ATCGCGC509.070738E-625.9000028
CGAACCG659.358155E-825.61538531
GCGAACC659.358155E-825.61538530
TCGCGCG451.320995E-424.6666689
GGCGAAC802.7139322E-823.12500229
CTCTAAT1155.456968E-1222.521741
CCACTTA502.6976122E-422.214
GTATCGC502.6976122E-422.26
CAAGACA502.6976122E-422.24
GAACCGG753.7275095E-722.232
CTAATAC1450.021.6896553
GCGCGCC603.7180616E-521.58333411
CGCAAGG705.0874914E-621.14285710
CCGCAAG705.0874914E-621.1428579
ATAGGTT806.93557E-720.812517
TAGGTTA806.93557E-720.812518
TAATACT1700.020.6764724
Produced by FastQC (version 0.11.5)