##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632966.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	386779
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.739794559683954	33.0	33.0	33.0	27.0	33.0
2	31.254248550205673	33.0	33.0	33.0	27.0	33.0
3	31.6743644303336	33.0	33.0	33.0	27.0	33.0
4	31.804451637756962	33.0	33.0	33.0	27.0	33.0
5	31.91352684608006	33.0	33.0	33.0	33.0	33.0
6	34.77078900353949	37.0	37.0	37.0	27.0	37.0
7	34.937072591841854	37.0	37.0	37.0	27.0	37.0
8	35.120179740885625	37.0	37.0	37.0	33.0	37.0
9	35.20323492226827	37.0	37.0	37.0	33.0	37.0
10	35.225027729013206	37.0	37.0	37.0	33.0	37.0
11	35.25362804081917	37.0	37.0	37.0	33.0	37.0
12	35.20767932074906	37.0	37.0	37.0	33.0	37.0
13	35.245832891651304	37.0	37.0	37.0	33.0	37.0
14	35.22645490060215	37.0	37.0	37.0	33.0	37.0
15	35.23729571667541	37.0	37.0	37.0	33.0	37.0
16	35.25181563631945	37.0	37.0	37.0	33.0	37.0
17	35.19080405089211	37.0	37.0	37.0	33.0	37.0
18	35.21913030438571	37.0	37.0	37.0	33.0	37.0
19	35.21281403592232	37.0	37.0	37.0	33.0	37.0
20	35.229735843983256	37.0	37.0	37.0	33.0	37.0
21	35.22146755640818	37.0	37.0	37.0	33.0	37.0
22	35.20714154594743	37.0	37.0	37.0	33.0	37.0
23	35.22091426887189	37.0	37.0	37.0	33.0	37.0
24	35.20222659451522	37.0	37.0	37.0	33.0	37.0
25	35.1960370133849	37.0	37.0	37.0	33.0	37.0
26	35.14687198632811	37.0	37.0	37.0	33.0	37.0
27	35.09290059698174	37.0	37.0	37.0	33.0	37.0
28	35.07870127385406	37.0	37.0	37.0	33.0	37.0
29	35.117390034102165	37.0	37.0	37.0	33.0	37.0
30	35.06566023491451	37.0	37.0	37.0	33.0	37.0
31	35.069838331450256	37.0	37.0	37.0	27.0	37.0
32	35.11535528040561	37.0	37.0	37.0	33.0	37.0
33	35.071914452439245	37.0	37.0	37.0	33.0	37.0
34	35.01003415387081	37.0	37.0	37.0	27.0	37.0
35	35.00935159354567	37.0	37.0	37.0	27.0	37.0
36	34.99529447048573	37.0	37.0	37.0	27.0	37.0
37	35.02604588149822	37.0	37.0	37.0	27.0	37.0
38	34.88880730339548	37.0	37.0	37.0	27.0	37.0
39	34.955861098973834	37.0	37.0	37.0	27.0	37.0
40	34.98414857063077	37.0	37.0	37.0	27.0	37.0
41	34.99348982235333	37.0	37.0	37.0	27.0	37.0
42	34.927253030800536	37.0	37.0	37.0	27.0	37.0
43	34.711494160748124	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	11.0
16	16.0
17	11.0
18	15.0
19	19.0
20	21.0
21	69.0
22	207.0
23	520.0
24	1101.0
25	1972.0
26	3294.0
27	4975.0
28	6951.0
29	9351.0
30	11929.0
31	15541.0
32	19416.0
33	25201.0
34	36238.0
35	65171.0
36	184749.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.847357276377465	20.60401417863948	13.394212198697447	25.154416346285608
2	16.001902895451924	22.795963586440834	37.88701041163041	23.315123106476825
3	17.894715069846086	26.593222486225987	30.05152813363704	25.46053431029089
4	12.80912355634613	17.890319795025068	38.88189379464759	30.418662853981214
5	14.078582342888316	36.96555397268207	35.33154592157278	13.624317762856824
6	29.923030981516575	39.576605761946745	17.42545484630758	13.074908410229098
7	26.872451710149726	31.85643481161076	23.618655614705037	17.652457863534472
8	24.33094868128828	36.18629760147268	20.63426401123122	18.848489706007822
9	25.76897918449554	15.483001920993642	20.244118734471105	38.50390016003971
10	15.061831174908669	28.07443010091034	33.88627614218972	22.97746258199127
11	34.27926542030462	23.50463701493618	24.108858030037826	18.10723953472138
12	23.1589615775417	25.55283508153235	30.16632237013902	21.121880970786936
13	28.08606465190716	22.23517822839399	25.9372923555829	23.741464764115943
14	21.94896827387216	20.772327349726847	27.514161834018907	29.764542542382085
15	24.003888525488716	28.632371457602403	24.10653111983846	23.25720889707042
16	23.25720889707042	28.239640725065218	25.125200696004697	23.37794968185967
17	22.642904604438193	27.473311632741176	26.28736306779841	23.59642069502222
18	23.17757685913661	27.464779628676837	27.383854862854502	21.973788649332047
19	24.77332016474524	26.286070339909873	26.937346650154222	22.003262845190662
20	24.181509337373537	26.2871045222207	27.200028957104706	22.33135718330106
21	23.322620928230332	27.024735055419246	26.997329224182288	22.655314792168138
22	23.024776422711675	26.954410658282896	26.905286998518534	23.115525920486892
23	22.972808761592535	26.797473492614643	27.529933114259048	22.69978463153377
24	23.264189627668515	27.18787731495247	26.004514205786766	23.54341885159225
25	23.493519555094768	26.80807386130064	26.89804772234273	22.800358861261856
26	23.288492911972988	27.17184748913462	26.891842628477765	22.64781697041463
27	23.270653267111193	26.970957575256154	26.444558779044364	23.31383037858829
28	23.022190966934605	26.760501475002517	27.290261363724504	22.92704619433837
29	24.03362126692504	26.756364745759203	26.53530827681958	22.674705710496173
30	23.669072002357936	26.60278867260115	27.05446779685557	22.673671528185345
31	23.557897403943855	26.731027279143905	26.733612734920975	22.97746258199127
32	22.356953195494068	26.593998122959107	27.34119484253282	23.70785383901401
33	22.682979168982804	26.523673725822754	27.69307537379227	23.10027173140217
34	23.301678736436052	26.264611056960174	27.237259520294536	23.196450686309237
35	23.063299713790045	27.09428381582247	26.976128486810296	22.866287983577184
36	23.964072506521813	26.68293780169037	26.543064644150792	22.809925047637023
37	23.173957221048713	26.318388537123266	26.83625532927072	23.6713989125573
38	23.316157288787654	26.685006166312032	26.746540013806335	23.252296531093982
39	22.891883995770193	26.68552325746744	27.074892897494436	23.34769984926793
40	23.048045524705323	27.137719472877276	27.218902784277326	22.595332218140072
41	23.05399207299259	26.04355458802055	27.340160660221986	23.562292678764877
42	22.791568311619812	27.052140886656208	27.063775437653025	23.092515364070955
43	23.53178430059543	25.957458910644064	27.080839445781702	23.429917342978808
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	14.0
2	19.0
3	67.0
4	115.0
5	115.0
6	134.0
7	153.0
8	134.5
9	116.0
10	174.5
11	233.0
12	233.0
13	381.5
14	530.0
15	935.5
16	1341.0
17	1635.0
18	1929.0
19	1929.0
20	1929.0
21	1929.0
22	2284.0
23	2639.0
24	3569.0
25	4499.0
26	4499.0
27	5975.5
28	7452.0
29	9158.0
30	10864.0
31	13065.5
32	15267.0
33	15267.0
34	17891.0
35	20515.0
36	22345.5
37	24176.0
38	26371.0
39	28566.0
40	28566.0
41	29603.0
42	30640.0
43	31342.0
44	32044.0
45	31115.5
46	30187.0
47	30187.0
48	29337.0
49	28487.0
50	28409.0
51	28331.0
52	26904.5
53	25478.0
54	25478.0
55	24558.5
56	23639.0
57	20881.0
58	18123.0
59	16710.5
60	15298.0
61	15298.0
62	13506.5
63	11715.0
64	9822.0
65	7929.0
66	6645.0
67	5361.0
68	5361.0
69	4485.0
70	3609.0
71	2946.5
72	2284.0
73	1860.5
74	1437.0
75	1437.0
76	1107.0
77	777.0
78	623.0
79	469.0
80	358.5
81	248.0
82	248.0
83	200.0
84	152.0
85	130.0
86	108.0
87	89.0
88	70.0
89	70.0
90	53.5
91	37.0
92	20.5
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	386779.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.74089204560535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.53723807679863	59.97591486576476
2	6.760434479095425	9.159157244595828
3	1.7251295234982384	3.505854384479423
4	0.8306121609301579	2.2506563490134726
5	0.4515528435890225	1.5294296215225045
6	0.29443486429816546	1.1967168214130692
7	0.21916584531385389	1.0392542907242734
8	0.15389656695160583	0.8340072582446407
9	0.12338272481097726	0.7522250257541739
>10	0.7758005021014752	10.054115977955865
>50	0.08492779397552228	4.005812879842673
>100	0.04265604131594427	5.249624547175199
>500	3.842886605040024E-4	0.17936087042565269
>1k	3.842886605040024E-4	0.26786986308852917
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1029	0.2660433994606739	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	689	0.17813790304023744	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	457	0.1181553290121749	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	430	0.11117459841408144	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.585455777071661E-4	0.0	0.0	0.0	0.0
3	2.585455777071661E-4	0.0	0.0	0.0	0.0
4	2.585455777071661E-4	0.0	0.0	0.0	0.0
5	2.585455777071661E-4	0.0	2.585455777071661E-4	0.0	0.0
6	2.585455777071661E-4	0.0	5.170911554143322E-4	0.0	0.0
7	2.585455777071661E-4	0.0	0.0018098190439501628	0.0	0.0
8	2.585455777071661E-4	0.0	0.0018098190439501628	0.0	0.0
9	2.585455777071661E-4	0.0	0.002326910199364495	2.585455777071661E-4	0.0
10	5.170911554143322E-4	0.0	0.002844001354778827	2.585455777071661E-4	0.0
11	5.170911554143322E-4	0.0	0.005170911554143322	5.170911554143322E-4	0.0
12	5.170911554143322E-4	0.0	0.005170911554143322	5.170911554143322E-4	0.0
13	5.170911554143322E-4	0.0	0.005429457131850488	5.170911554143322E-4	0.0
14	5.170911554143322E-4	0.0	0.005688002709557654	5.170911554143322E-4	0.0
15	5.170911554143322E-4	0.0	0.005946548287264821	5.170911554143322E-4	0.0
16	5.170911554143322E-4	0.0	0.006722185020386319	5.170911554143322E-4	0.0
17	5.170911554143322E-4	0.0	0.006722185020386319	5.170911554143322E-4	0.0
18	5.170911554143322E-4	0.0	0.006722185020386319	5.170911554143322E-4	0.0
19	5.170911554143322E-4	0.0	0.0069807305980934846	5.170911554143322E-4	0.0
20	5.170911554143322E-4	0.0	0.0069807305980934846	5.170911554143322E-4	0.0
21	5.170911554143322E-4	0.0	0.007239276175800651	5.170911554143322E-4	0.0
22	5.170911554143322E-4	0.0	0.007497821753507817	5.170911554143322E-4	0.0
23	5.170911554143322E-4	0.0	0.007497821753507817	5.170911554143322E-4	0.0
24	5.170911554143322E-4	0.0	0.007497821753507817	7.756367331214983E-4	0.0
25	5.170911554143322E-4	0.0	0.007497821753507817	0.0010341823108286645	0.0
26	5.170911554143322E-4	0.0	0.007497821753507817	0.0015512734662429966	0.0
27	5.170911554143322E-4	0.0	0.007497821753507817	0.002585455777071661	0.0
28	5.170911554143322E-4	0.0	0.007497821753507817	0.008790549642043647	0.0
29	5.170911554143322E-4	0.0	0.007497821753507817	0.017581099284087295	0.0
30	5.170911554143322E-4	0.0	0.007497821753507817	0.036972017612124757	0.0
31	5.170911554143322E-4	0.0	0.007497821753507817	0.09204222566375113	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACCG	25	0.005491891	29.6	5
GGTATCA	245	0.0	28.693876	1
TATCGCG	40	5.9300968E-5	27.750002	7
ATCGCGC	50	9.070738E-6	25.900002	8
CGAACCG	65	9.358155E-8	25.615385	31
GCGAACC	65	9.358155E-8	25.615385	30
TCGCGCG	45	1.320995E-4	24.666668	9
GGCGAAC	80	2.7139322E-8	23.125002	29
CTCTAAT	115	5.456968E-12	22.52174	1
CCACTTA	50	2.6976122E-4	22.2	14
GTATCGC	50	2.6976122E-4	22.2	6
CAAGACA	50	2.6976122E-4	22.2	4
GAACCGG	75	3.7275095E-7	22.2	32
CTAATAC	145	0.0	21.689655	3
GCGCGCC	60	3.7180616E-5	21.583334	11
CGCAAGG	70	5.0874914E-6	21.142857	10
CCGCAAG	70	5.0874914E-6	21.142857	9
ATAGGTT	80	6.93557E-7	20.8125	17
TAGGTTA	80	6.93557E-7	20.8125	18
TAATACT	170	0.0	20.676472	4
>>END_MODULE