FastQCFastQC Report
Fri 10 Feb 2017
ERR1632965.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632965.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400700
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8840.22061392563014726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6480.161716995258298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGAA250.005492124729.637
CTTAGAC358.8592735E-426.4285723
GTATCAA6400.021.3906251
GGTATCA2400.020.8124981
GTTACAC450.003821524820.5555573
ATAAGGT450.003821524820.5555574
TTAGACA555.135061E-420.1818184
GCCGAGT656.8878E-519.92307712
CCGAGTT500.007027082618.513
ATACAAC500.007027082618.53
TGCCGAG500.007027082618.511
GTATTAG1351.1441443E-917.8148161
AAATGTG752.0630348E-417.2666665
GTTAGCC650.001577507117.0769233
TATTAGA1201.0361873E-716.9583322
TTAACGG1201.0361873E-716.95833235
AACAGTG904.4343382E-516.4444457
ATTAGAG1251.649696E-716.2799993
ATCGGTC803.3756922E-416.187514
CCTCTAG803.3756922E-416.18751
Produced by FastQC (version 0.11.5)