##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632965.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	400700
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71689293735962	33.0	33.0	33.0	27.0	33.0
2	31.236338906912902	33.0	33.0	33.0	27.0	33.0
3	31.669021712003993	33.0	33.0	33.0	27.0	33.0
4	31.790995757424508	33.0	33.0	33.0	27.0	33.0
5	31.90143998003494	33.0	33.0	33.0	33.0	33.0
6	34.7623284252558	37.0	37.0	37.0	27.0	37.0
7	34.929066633391564	37.0	37.0	37.0	27.0	37.0
8	35.10599450960819	37.0	37.0	37.0	33.0	37.0
9	35.19050910905915	37.0	37.0	37.0	33.0	37.0
10	35.226703269278765	37.0	37.0	37.0	33.0	37.0
11	35.24447716496132	37.0	37.0	37.0	33.0	37.0
12	35.213356625904666	37.0	37.0	37.0	33.0	37.0
13	35.24426753181932	37.0	37.0	37.0	33.0	37.0
14	35.21645620164712	37.0	37.0	37.0	33.0	37.0
15	35.23285999500874	37.0	37.0	37.0	33.0	37.0
16	35.23542300973297	37.0	37.0	37.0	33.0	37.0
17	35.18513601197904	37.0	37.0	37.0	33.0	37.0
18	35.206843024706764	37.0	37.0	37.0	33.0	37.0
19	35.210012478163215	37.0	37.0	37.0	33.0	37.0
20	35.215046169203895	37.0	37.0	37.0	33.0	37.0
21	35.21440728724732	37.0	37.0	37.0	33.0	37.0
22	35.21507861242825	37.0	37.0	37.0	33.0	37.0
23	35.19047916146743	37.0	37.0	37.0	33.0	37.0
24	35.197329673072126	37.0	37.0	37.0	33.0	37.0
25	35.19983528824557	37.0	37.0	37.0	33.0	37.0
26	35.16492637883704	37.0	37.0	37.0	33.0	37.0
27	35.10293486398802	37.0	37.0	37.0	33.0	37.0
28	35.06277264786623	37.0	37.0	37.0	33.0	37.0
29	35.10651859246319	37.0	37.0	37.0	33.0	37.0
30	35.07326927876217	37.0	37.0	37.0	33.0	37.0
31	35.06638881956576	37.0	37.0	37.0	27.0	37.0
32	35.10002745195907	37.0	37.0	37.0	33.0	37.0
33	35.08331669578238	37.0	37.0	37.0	33.0	37.0
34	35.00732967307212	37.0	37.0	37.0	27.0	37.0
35	35.01357624157724	37.0	37.0	37.0	27.0	37.0
36	34.99233840778638	37.0	37.0	37.0	27.0	37.0
37	35.01592962315947	37.0	37.0	37.0	27.0	37.0
38	34.885410531569754	37.0	37.0	37.0	27.0	37.0
39	34.954509608185674	37.0	37.0	37.0	27.0	37.0
40	34.98477414524582	37.0	37.0	37.0	27.0	37.0
41	35.01061642126279	37.0	37.0	37.0	27.0	37.0
42	34.94333915647616	37.0	37.0	37.0	27.0	37.0
43	34.7214898926878	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	8.0
16	30.0
17	20.0
18	14.0
19	15.0
20	34.0
21	81.0
22	236.0
23	522.0
24	1148.0
25	2134.0
26	3374.0
27	5122.0
28	7246.0
29	9746.0
30	12379.0
31	15963.0
32	20347.0
33	26229.0
34	37168.0
35	67386.0
36	191496.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.72198652358373	20.547791365111056	13.49238832043923	25.23783379086598
2	16.0319440978288	22.648864487147492	37.94235088594959	23.37684052907412
3	17.87471924132768	26.470925879710506	30.00449213875718	25.649862740204643
4	12.947591714499627	17.99026703269279	38.884951335163464	30.17718991764412
5	14.066383828300474	36.92063888195658	35.403294235088595	13.609683054654356
6	29.954829049163962	39.563014724232595	17.415273271774396	13.066882954829047
7	26.607936111804342	32.15947092587971	23.81257798852009	17.42001497379586
8	24.26303968055902	36.564512103818316	20.549039181432494	18.623409034190168
9	25.805839780384325	15.51035687546793	20.35512852508111	38.328674819066634
10	14.738956825555277	28.209882705265787	34.26927876216621	22.781881707012726
11	34.249812827551786	23.61716995258298	24.2934863988021	17.83953082106314
12	22.870726229099077	25.604442226104318	30.474918891939108	21.0499126528575
13	28.01247816321438	22.122036436236588	26.0778637384577	23.78762166209134
14	21.8442725230846	20.830546543548788	27.460194659346143	29.864986274020467
15	23.768405290741203	28.887696531070628	23.97379585724981	23.370102320938358
16	23.206887946094334	28.251310207137507	25.041677065136014	23.500124781632145
17	22.449213875717493	27.576491140504118	26.59720489143998	23.377090092338406
18	23.214125280758672	27.330421761916647	27.409533316695782	22.0459196406289
19	24.557524332418268	26.578986773146994	26.937609183928124	21.925879710506614
20	24.030696281507364	26.603693536311457	27.136011979036684	22.229598203144498
21	23.34988769653107	26.981532318442724	27.083853256800598	22.584726728225604
22	22.955577738956826	26.957574245071125	27.03968055902171	23.047167456950337
23	23.05864736710756	26.799101572248563	27.424257549288743	22.71799351135513
24	23.070376840529075	27.20863488894435	26.27926129273771	23.441726977788868
25	23.379336161716996	26.978787122535564	27.08485150985775	22.557025205889694
26	23.171949089094085	27.20064886448715	27.023708510107312	22.603693536311457
27	23.099326179186424	27.220114799101573	26.67781382580484	23.002745195907163
28	22.848764661841777	26.907162465685055	27.41801846768156	22.826054404791616
29	23.780384327427004	26.859995008734717	26.696531070626406	22.66308959321188
30	23.511854255053656	26.70277015223359	27.041926628400297	22.743448964312453
31	23.469178936860494	26.636885450461694	27.033441477414527	22.86049413526329
32	22.38807087596706	26.712752682805093	27.489143998003495	23.410032443224356
33	22.73970551534814	26.874968804591965	27.459445969553283	22.925879710506614
34	23.24931370102321	26.41602196156726	27.181182929872723	23.15348140753681
35	23.01472423259296	27.153481407536812	27.18143249313701	22.650361866733217
36	23.73371599700524	26.76341402545545	26.66209134015473	22.840778637384577
37	23.050411779386074	26.554529573246814	26.897179935113552	23.497878712253556
38	23.190915897179934	27.037434489643125	26.635637634140252	23.136011979036684
39	22.76566009483404	26.729223858248062	27.376590965809832	23.128525081108062
40	22.995507861242825	27.0279510856002	27.437983528824557	22.53855752433242
41	22.998253057149988	26.318692288495132	27.35487896181682	23.328175692538057
42	22.66408784626903	27.089842775143495	27.23633641128026	23.00973296730721
43	23.27402046418767	26.147741452458202	27.260544047916145	23.317694035437984
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	10.0
2	16.0
3	52.0
4	88.0
5	88.0
6	126.5
7	165.0
8	142.5
9	120.0
10	174.0
11	228.0
12	228.0
13	328.5
14	429.0
15	839.5
16	1250.0
17	1493.0
18	1736.0
19	1736.0
20	1796.0
21	1856.0
22	2324.5
23	2793.0
24	3833.5
25	4874.0
26	4874.0
27	6495.0
28	8116.0
29	9964.5
30	11813.0
31	14118.5
32	16424.0
33	16424.0
34	18860.5
35	21297.0
36	23426.0
37	25555.0
38	27654.0
39	29753.0
40	29753.0
41	31116.5
42	32480.0
43	33013.5
44	33547.0
45	32852.0
46	32157.0
47	32157.0
48	30987.5
49	29818.0
50	29656.5
51	29495.0
52	27768.5
53	26042.0
54	26042.0
55	24797.5
56	23553.0
57	20641.5
58	17730.0
59	16349.5
60	14969.0
61	14969.0
62	13242.5
63	11516.0
64	9793.5
65	8071.0
66	6749.0
67	5427.0
68	5427.0
69	4569.0
70	3711.0
71	3013.5
72	2316.0
73	1893.0
74	1470.0
75	1470.0
76	1124.5
77	779.0
78	617.0
79	455.0
80	356.0
81	257.0
82	257.0
83	204.0
84	151.0
85	134.5
86	118.0
87	97.0
88	76.0
89	76.0
90	56.5
91	37.0
92	21.5
93	6.0
94	4.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	400700.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.94820848652812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.1479374019762	62.35738511537389
2	6.2269069006574895	8.711219642267814
3	1.6817507105222034	3.529063479659216
4	0.7702682971299429	2.1551554975283294
5	0.45736751922466873	1.5996019294846644
6	0.30244523843164695	1.2693301556144727
7	0.19782285972534758	0.9686148244828873
8	0.1618809735661316	0.9058627271204736
9	0.13135846852963812	0.8269460588863952
>10	0.8307629287908738	11.099453455023589
>50	0.06279322648908929	2.9955306618066486
>100	0.027987838082897225	3.197324264004115
>500	7.176368739204417E-4	0.38451218874749227
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	884	0.22061392563014726	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	648	0.161716995258298	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	2.495632642874969E-4	2.495632642874969E-4	0.0
5	0.0	0.0	9.982530571499876E-4	2.495632642874969E-4	0.0
6	0.0	0.0	0.001996506114299975	2.495632642874969E-4	0.0
7	0.0	0.0	0.0029947591714499623	2.495632642874969E-4	0.0
8	0.0	0.0	0.0029947591714499623	2.495632642874969E-4	0.0
9	2.495632642874969E-4	0.0	0.0032443224357374596	2.495632642874969E-4	0.0
10	4.991265285749938E-4	0.0	0.0034938857000249564	4.991265285749938E-4	0.0
11	4.991265285749938E-4	0.0	0.00399301222859995	4.991265285749938E-4	0.0
12	4.991265285749938E-4	0.0	0.004242575492887447	4.991265285749938E-4	0.0
13	4.991265285749938E-4	0.0	0.005739955078612428	4.991265285749938E-4	0.0
14	4.991265285749938E-4	0.0	0.005739955078612428	4.991265285749938E-4	0.0
15	4.991265285749938E-4	0.0	0.006488644871474919	4.991265285749938E-4	0.0
16	4.991265285749938E-4	0.0	0.007486897928624906	4.991265285749938E-4	0.0
17	4.991265285749938E-4	0.0	0.007736461192912403	7.486897928624906E-4	0.0
18	4.991265285749938E-4	0.0	0.007736461192912403	0.0012478163214374844	0.0
19	4.991265285749938E-4	0.0	0.0079860244571999	0.0012478163214374844	0.0
20	4.991265285749938E-4	0.0	0.0079860244571999	0.0012478163214374844	0.0
21	4.991265285749938E-4	0.0	0.0079860244571999	0.0014973795857249812	0.0
22	4.991265285749938E-4	0.0	0.0079860244571999	0.0017469428500124782	0.0
23	4.991265285749938E-4	0.0	0.0079860244571999	0.0017469428500124782	0.0
24	4.991265285749938E-4	0.0	0.0079860244571999	0.0017469428500124782	0.0
25	4.991265285749938E-4	0.0	0.0079860244571999	0.0017469428500124782	0.0
26	4.991265285749938E-4	0.0	0.0079860244571999	0.001996506114299975	0.0
27	4.991265285749938E-4	0.0	0.0079860244571999	0.0024956326428749688	0.0
28	4.991265285749938E-4	0.0	0.0079860244571999	0.0072373346643374095	0.0
29	4.991265285749938E-4	0.0	0.0079860244571999	0.02071375093586224	0.0
30	4.991265285749938E-4	0.0	0.0079860244571999	0.037434489643124534	0.0
31	4.991265285749938E-4	0.0	0.0079860244571999	0.09333666084352384	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCCGAA	25	0.0054921247	29.6	37
CTTAGAC	35	8.8592735E-4	26.428572	3
GTATCAA	640	0.0	21.390625	1
GGTATCA	240	0.0	20.812498	1
GTTACAC	45	0.0038215248	20.555557	3
ATAAGGT	45	0.0038215248	20.555557	4
TTAGACA	55	5.135061E-4	20.181818	4
GCCGAGT	65	6.8878E-5	19.923077	12
CCGAGTT	50	0.0070270826	18.5	13
ATACAAC	50	0.0070270826	18.5	3
TGCCGAG	50	0.0070270826	18.5	11
GTATTAG	135	1.1441443E-9	17.814816	1
AAATGTG	75	2.0630348E-4	17.266666	5
GTTAGCC	65	0.0015775071	17.076923	3
TATTAGA	120	1.0361873E-7	16.958332	2
TTAACGG	120	1.0361873E-7	16.958332	35
AACAGTG	90	4.4343382E-5	16.444445	7
ATTAGAG	125	1.649696E-7	16.279999	3
ATCGGTC	80	3.3756922E-4	16.1875	14
CCTCTAG	80	3.3756922E-4	16.1875	1
>>END_MODULE