FastQCFastQC Report
Fri 10 Feb 2017
ERR1632964.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632964.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424957
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18650.43886793252023143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13490.3174438825575293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6980.1642519125464459No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT6580.15483919549507363No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6180.14542647844370135No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC6000.14119075577058385No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA5210.12260063959412364No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA5170.12165936788898642No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG5110.12024746033128057No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC4980.1171883272895846No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT4640.10918751779591819No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC4620.10871688194334955No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC4410.10377520549137913No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCGC200.001840630937.025
ATAGACC303.5956944E-430.8333343
ATGTTCG250.005492492629.624
TCGCCAT1100.026.9090913
ATAGGAC358.8601594E-426.4285723
GGTATCA2850.025.9649121
TTAACGG809.749783E-1025.437535
GTATCAA8650.024.8092481
GTCGCCA1200.024.66666812
TAACGGC901.4188117E-1024.66666636
AACGGCC901.4188117E-1024.66666637
CGAGTCG802.7155693E-823.12521
TTTAACG957.1122486E-921.42105334
CGTCCGC705.089445E-621.14285929
CCGTCCG806.938899E-720.812528
CGGGATA450.003821900320.55555517
CGGTTGG555.135766E-420.18181833
CGCTCTC759.243255E-619.73333229
TTCGCCG851.2415312E-619.58823624
GCCGTCC851.2415312E-619.58823627
Produced by FastQC (version 0.11.5)