FastQCFastQC Report
Fri 10 Feb 2017
ERR1632963.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632963.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311630
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64122.057568270063858No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49881.6006161152648974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25260.81057664538074No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG12790.4104226165645156No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12640.4056092160575041No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10120.3247440875397105No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9840.3157590732599557No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9190.2949010043962391No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9100.2920129640920322No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG8650.27757276257099767No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7910.25382665340307414No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7720.24772967942752622No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7710.24740878606039213No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6220.19959567435741105No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA6130.19670763405320413No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5850.18772261977344928No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5650.18130475243076724No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5310.1703943779482078No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5000.16044668356705066No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4880.15659596316144145No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC4820.15467060295863685No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4770.15306613612296632No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4770.15306613612296632No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4770.15306613612296632No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4760.15274524275583223No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4750.15242434938869814No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.15081988255302764No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4690.15049898918589352No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4480.14376022847607742No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4210.13509610756345666No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4180.13413342746205437No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4070.13060360042357924No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4060.13028270705644515No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC3950.12675288001797003No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT3920.12579019991656773No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT3880.12450662644803133No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3880.12450662644803133No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3810.1222603728780926No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3770.1209767994095562No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG3750.12033501267528801No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.11840965247248339No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3680.11808875910534929No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC3650.117126079003947No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3640.11680518563681287No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3610.11584250553541059No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG3580.11487982543400828No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT3570.11455893206687418No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3460.11102910502839905No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3430.11006642492699677No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC3390.10878285145846035No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC3380.10846195809132626No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3380.10846195809132626No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA3340.10717838462278985No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3320.10653659788852163No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA3230.10364855758431472No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3210.10300677085004653No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3160.10140230401437603No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT3160.10140230401437603No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG3150.10108141064724192No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT3120.10011873054583961No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3120.10011873054583961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9800.026.9948981
CGGGCTA509.063353E-625.89999817
GCCGGCA3050.025.4754115
AGCTTCG2950.024.45762421
GCCGCTC2650.024.43396227
GTATCAA28000.024.3803581
GCTTCGC3000.024.05000122
ACTAGTG1002.0008883E-1124.058
CGCTCTC2700.023.98148229
GTATTAT701.9135769E-723.7857151
GCCGTCC1950.023.71794927
CCGCTCT2750.023.54545428
TCGCCGT2300.023.32608825
TTCGCCG2950.023.20338824
CGCCGGC3350.023.1940314
CAGCTTC3050.023.04918120
GCAGCTT3150.022.9047619
GGCAGCT3250.022.76923218
TCCGCCG1950.022.76923231
CCGGCAG3250.022.76923216
Produced by FastQC (version 0.11.5)