##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632963.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	311630
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.779632897987998	33.0	33.0	33.0	33.0	33.0
2	31.270420049417577	33.0	33.0	33.0	27.0	33.0
3	31.699486570612585	33.0	33.0	33.0	27.0	33.0
4	31.811824920578893	33.0	33.0	33.0	27.0	33.0
5	31.93431633668132	33.0	33.0	33.0	33.0	33.0
6	34.71529698681128	37.0	37.0	37.0	27.0	37.0
7	34.92781824599685	37.0	37.0	37.0	27.0	37.0
8	35.13234284247345	37.0	37.0	37.0	33.0	37.0
9	35.226762506818986	37.0	37.0	37.0	33.0	37.0
10	35.162654429932935	37.0	37.0	37.0	33.0	37.0
11	35.21984404582357	37.0	37.0	37.0	33.0	37.0
12	35.17258287071206	37.0	37.0	37.0	33.0	37.0
13	35.19506466001348	37.0	37.0	37.0	33.0	37.0
14	35.180078939768315	37.0	37.0	37.0	33.0	37.0
15	35.216307800917754	37.0	37.0	37.0	33.0	37.0
16	35.244039405705486	37.0	37.0	37.0	33.0	37.0
17	35.211763950839135	37.0	37.0	37.0	33.0	37.0
18	35.203006770850045	37.0	37.0	37.0	33.0	37.0
19	35.190633122613356	37.0	37.0	37.0	33.0	37.0
20	35.18390077977088	37.0	37.0	37.0	33.0	37.0
21	35.1783332798511	37.0	37.0	37.0	33.0	37.0
22	35.17464942399641	37.0	37.0	37.0	33.0	37.0
23	35.1618072714437	37.0	37.0	37.0	33.0	37.0
24	35.157420659114976	37.0	37.0	37.0	33.0	37.0
25	35.135789237236466	37.0	37.0	37.0	33.0	37.0
26	35.116705708693004	37.0	37.0	37.0	33.0	37.0
27	35.04144337836537	37.0	37.0	37.0	27.0	37.0
28	35.00273080255431	37.0	37.0	37.0	27.0	37.0
29	35.039810031126656	37.0	37.0	37.0	27.0	37.0
30	34.98966402464461	37.0	37.0	37.0	27.0	37.0
31	34.95427269518339	37.0	37.0	37.0	27.0	37.0
32	34.95762282193627	37.0	37.0	37.0	27.0	37.0
33	34.904511760741904	37.0	37.0	37.0	27.0	37.0
34	34.80915508776434	37.0	37.0	37.0	27.0	37.0
35	34.77548695568463	37.0	37.0	37.0	27.0	37.0
36	34.716570933478806	37.0	37.0	37.0	27.0	37.0
37	34.69301094246382	37.0	37.0	37.0	27.0	37.0
38	34.479363347559605	37.0	37.0	37.0	27.0	37.0
39	34.52912428200109	37.0	37.0	37.0	27.0	37.0
40	34.49773770176171	37.0	37.0	37.0	27.0	37.0
41	34.42018419279273	37.0	37.0	37.0	27.0	37.0
42	34.25898661874659	37.0	37.0	37.0	27.0	37.0
43	33.98400988351571	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	6.0
16	23.0
17	17.0
18	19.0
19	23.0
20	31.0
21	67.0
22	201.0
23	510.0
24	955.0
25	1769.0
26	2841.0
27	4196.0
28	6041.0
29	8006.0
30	10873.0
31	13620.0
32	16848.0
33	21816.0
34	30036.0
35	52680.0
36	141047.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.55520970381542	17.668067901036487	14.78676635753939	20.989956037608703
2	16.15280942142926	25.63007412636781	38.427622501042904	19.78949395116003
3	19.36944453358149	29.508070468183423	30.43801944613805	20.684465552097038
4	11.860860636010653	18.029393832429484	39.528607643680004	30.581137887879855
5	15.776722395148093	35.49433623207008	30.155954176427173	18.572987196354653
6	26.122324551551518	41.8265250457273	15.521291274909347	16.52985912781183
7	24.594551230626063	34.26050123543946	20.853255463209575	20.2916920707249
8	25.634887526874824	32.05339665629111	19.435548567211118	22.87616724962295
9	29.097326958251774	12.811988576196129	18.156467605814587	39.93421685973751
10	17.699194557648493	26.533068061483167	28.999133587908737	26.7686037929596
11	35.46673940249655	25.71318550845554	19.96085100920964	18.85922407983827
12	25.065622693578927	26.859737509225685	27.91451400699548	20.160125790199917
13	32.47087892693258	23.385745916631905	24.35388120527549	19.78949395116003
14	24.394634662901517	21.232551423162082	29.507428681449156	24.865385232487245
15	26.483650482944515	25.604723550364213	26.767641112858197	21.14398485383307
16	20.63665244039406	28.430831434714243	25.358277444405225	25.574238680486474
17	22.30818598979559	24.32371722876488	25.757468793120047	27.61062798831948
18	26.776947020505087	21.315341911882683	28.66668805955781	23.241023008054423
19	29.21926643776273	22.337708179571926	28.882328402271924	19.560696980393416
20	28.4680550653018	21.281969001700734	28.398742098000834	21.85123383499663
21	24.571446908192407	24.84773609729487	28.616307800917756	21.96450919359497
22	23.394410037544525	24.478066938356385	30.063536886692553	22.06398613740654
23	22.846003273112345	24.904534223277604	30.24548342585759	22.003979077752465
24	22.927510188364405	23.37676090235215	30.888232840227193	22.807496069056253
25	22.867824022077464	24.722266790745433	31.228058916022206	21.181850271154897
26	23.748676314860575	24.92282514520425	29.10021499855598	22.2282835413792
27	23.41430542630684	24.43635080062895	31.12152231813368	21.027821454930525
28	22.145813946025736	25.518724127972277	29.361743092770272	22.973718833231718
29	22.43461797644643	24.829124282001093	28.931745980810575	23.804511760741907
30	23.75477328883612	24.719057857074095	30.1655809774412	21.36058787664859
31	23.92388409331579	24.53871578474473	28.866604627282356	22.670795494657124
32	20.69473413984533	25.007861887494787	30.991881397811504	23.30552257484838
33	21.434393351089433	25.21676346949909	30.63248082662131	22.716362352790167
34	21.090074768154544	24.695632641273306	30.349452876809035	23.864839713763118
35	22.039277348137215	24.988929178833875	29.856560664891056	23.115232808137858
36	22.44424477746045	25.3204120270834	30.277572762570998	21.95777043288515
37	23.058755575522255	25.625581619227933	29.796874498604115	21.5187883066457
38	21.889099252318456	26.102429162789203	28.815582581908032	23.19288900298431
39	21.83101755286718	24.038443025382666	29.902127523024095	24.228411898726055
40	22.168276481725123	25.417000930590767	30.03497737701762	22.379745210666496
41	23.389596637037513	25.12338349966306	28.786702178865966	22.700317684433465
42	21.160671308924044	26.246831177999553	30.2098642621057	22.382633250970702
43	21.776786573821518	26.10307094952347	29.257131855084555	22.86301062157045
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	46.0
1	66.5
2	87.0
3	344.0
4	601.0
5	601.0
6	761.0
7	921.0
8	912.5
9	904.0
10	1313.5
11	1723.0
12	1723.0
13	2624.5
14	3526.0
15	6013.5
16	8501.0
17	8851.0
18	9201.0
19	9201.0
20	7064.5
21	4928.0
22	3575.0
23	2222.0
24	2246.5
25	2271.0
26	2271.0
27	2598.5
28	2926.0
29	3220.0
30	3514.0
31	4275.0
32	5036.0
33	5036.0
34	6627.0
35	8218.0
36	8901.5
37	9585.0
38	10597.5
39	11610.0
40	11610.0
41	13669.0
42	15728.0
43	17325.0
44	18922.0
45	21884.0
46	24846.0
47	24846.0
48	27465.5
49	30085.0
50	30472.0
51	30859.0
52	31084.5
53	31310.0
54	31310.0
55	27651.5
56	23993.0
57	21825.0
58	19657.0
59	17717.0
60	15777.0
61	15777.0
62	12763.5
63	9750.0
64	7263.5
65	4777.0
66	4594.5
67	4412.0
68	4412.0
69	3590.0
70	2768.0
71	2078.5
72	1389.0
73	1270.0
74	1151.0
75	1151.0
76	684.0
77	217.0
78	147.5
79	78.0
80	63.5
81	49.0
82	49.0
83	36.5
84	24.0
85	17.0
86	10.0
87	6.5
88	3.0
89	3.0
90	3.0
91	3.0
92	2.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	311630.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.01466919633048
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.43803650319244	41.10870361374261
2	6.268243081502517	5.893987498380562
3	1.9037755105753864	2.6851612756133085
4	0.9575294850346843	1.8007172793855344
5	0.629586857583014	1.4799908919811315
6	0.36833765375451644	1.0390363766292665
7	0.3105418835011596	1.0220016747088692
8	0.23877182812046915	0.898062280998955
9	0.17939169067739077	0.7590636894391174
>10	1.3415767334006439	12.794618606664429
>50	0.1930170087306371	6.341339187004304
>100	0.15891506372522418	15.709051717806474
>500	0.0081844668012991	2.862837594133011
>1k	0.0034101945005412908	3.5493670843927307
>5k	6.820389001082583E-4	2.0560612291197207
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6412	2.057568270063858	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4988	1.6006161152648974	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2526	0.81057664538074	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1279	0.4104226165645156	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1264	0.4056092160575041	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1012	0.3247440875397105	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	984	0.3157590732599557	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	919	0.2949010043962391	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	910	0.2920129640920322	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	865	0.27757276257099767	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	791	0.25382665340307414	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	772	0.24772967942752622	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	771	0.24740878606039213	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	622	0.19959567435741105	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	613	0.19670763405320413	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	585	0.18772261977344928	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	565	0.18130475243076724	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	531	0.1703943779482078	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	500	0.16044668356705066	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	488	0.15659596316144145	No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC	482	0.15467060295863685	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	477	0.15306613612296632	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	477	0.15306613612296632	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	477	0.15306613612296632	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	476	0.15274524275583223	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	475	0.15242434938869814	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	470	0.15081988255302764	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	469	0.15049898918589352	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	448	0.14376022847607742	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	421	0.13509610756345666	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	418	0.13413342746205437	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	407	0.13060360042357924	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	406	0.13028270705644515	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	395	0.12675288001797003	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	392	0.12579019991656773	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	388	0.12450662644803133	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	388	0.12450662644803133	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	381	0.1222603728780926	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	377	0.1209767994095562	No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG	375	0.12033501267528801	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	369	0.11840965247248339	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	368	0.11808875910534929	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	365	0.117126079003947	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	364	0.11680518563681287	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	361	0.11584250553541059	No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG	358	0.11487982543400828	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	357	0.11455893206687418	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	346	0.11102910502839905	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	343	0.11006642492699677	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	339	0.10878285145846035	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	338	0.10846195809132626	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	338	0.10846195809132626	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	334	0.10717838462278985	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	332	0.10653659788852163	No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA	323	0.10364855758431472	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	321	0.10300677085004653	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	316	0.10140230401437603	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	316	0.10140230401437603	No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG	315	0.10108141064724192	No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT	312	0.10011873054583961	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	312	0.10011873054583961	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0012835734685364053	0.0	0.0
2	3.208933671341013E-4	0.0	0.0016044668356705067	0.0	0.0
3	3.208933671341013E-4	0.0	0.0025671469370728106	0.0	0.0
4	3.208933671341013E-4	0.0	0.00481340050701152	0.0	0.0
5	3.208933671341013E-4	0.0	0.008664120912620737	0.0	0.0
6	3.208933671341013E-4	0.0	0.020537175496582485	0.0	0.0
7	3.208933671341013E-4	0.0	0.0481340050701152	6.417867342682026E-4	0.0
8	0.0012835734685364053	0.0	0.05679812598273594	6.417867342682026E-4	0.0
9	0.001925360202804608	0.0	0.06770850046529538	9.62680101402304E-4	0.0
10	0.0067387607098161285	0.0	0.07958155504925714	9.62680101402304E-4	0.0
11	0.0067387607098161285	0.0	0.11648429226967878	0.0012835734685364053	0.0
12	0.0067387607098161285	0.0	0.12867824022077465	0.0012835734685364053	0.0
13	0.0067387607098161285	0.0	0.13798414786766358	0.0012835734685364053	0.0
14	0.0067387607098161285	0.0	0.14793184224882072	0.0012835734685364053	0.0
15	0.0067387607098161285	0.0	0.15980489683278246	0.0016044668356705067	0.0
16	0.0070596540769502295	0.0	0.17328241825241472	0.001925360202804608	0.0
17	0.0070596540769502295	0.0	0.17905849886082856	0.002246253569938709	0.0
18	0.0070596540769502295	0.0	0.18483457946924237	0.002888040304206912	0.0
19	0.0070596540769502295	0.0	0.1874017264063152	0.002888040304206912	0.0
20	0.0070596540769502295	0.0	0.19028976671052208	0.002888040304206912	0.0
21	0.0070596540769502295	0.0	0.19702852742033822	0.0041716137727433175	0.0
22	0.0070596540769502295	0.0	0.19927478099027693	0.006417867342682027	0.0
23	0.0070596540769502295	0.0	0.20152103456021564	0.00962680101402304	0.0
24	0.0070596540769502295	0.0	0.20408818149728844	0.01187305458396175	0.0
25	0.0070596540769502295	0.0	0.20440907486442256	0.012835734685364053	0.0
26	0.0070596540769502295	0.0	0.20472996823155665	0.015402881622436864	0.0
27	0.0070596540769502295	0.0	0.20505086159869076	0.020537175496582485	0.0
28	0.0070596540769502295	0.0	0.20505086159869076	0.05230561884285852	0.0
29	0.0070596540769502295	0.0	0.20505086159869076	0.12611109328370182	0.0
30	0.0070596540769502295	0.0	0.20505086159869076	0.20665532843436127	0.0
31	0.0070596540769502295	0.0	0.20505086159869076	0.37737059974970316	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	980	0.0	26.994898	1
CGGGCTA	50	9.063353E-6	25.899998	17
GCCGGCA	305	0.0	25.47541	15
AGCTTCG	295	0.0	24.457624	21
GCCGCTC	265	0.0	24.433962	27
GTATCAA	2800	0.0	24.380358	1
GCTTCGC	300	0.0	24.050001	22
ACTAGTG	100	2.0008883E-11	24.05	8
CGCTCTC	270	0.0	23.981482	29
GTATTAT	70	1.9135769E-7	23.785715	1
GCCGTCC	195	0.0	23.717949	27
CCGCTCT	275	0.0	23.545454	28
TCGCCGT	230	0.0	23.326088	25
TTCGCCG	295	0.0	23.203388	24
CGCCGGC	335	0.0	23.19403	14
CAGCTTC	305	0.0	23.049181	20
GCAGCTT	315	0.0	22.90476	19
GGCAGCT	325	0.0	22.769232	18
TCCGCCG	195	0.0	22.769232	31
CCGGCAG	325	0.0	22.769232	16
>>END_MODULE