FastQCFastQC Report
Fri 10 Feb 2017
ERR1632962.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632962.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397358
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14290.35962532527343105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10710.2695302472833062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5160.12985771017571057No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA4230.10645312287659994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2800.029.7321411
CGAACTA601.3337303E-624.66666829
CGGCCTT601.3337303E-624.66666824
GCTTCGC451.3210849E-424.66666622
GTATCAA9050.024.3259661
GCGAACT652.675517E-622.7692328
TTCGCCG502.6977935E-422.19999924
CGAGGGT450.003821470320.55555516
AGACCGC450.003821470320.55555514
CGCCGGT450.003821470320.55555536
CTATCAC656.8876136E-519.9230773
TCTCGCC851.2411165E-619.58823412
TTCCTCG851.2411165E-619.58823419
AGCTTCG609.222322E-418.521
GCCGGCA609.222322E-418.515
CCTCGGC1103.8327926E-818.521
GACTGTG1103.8327926E-818.57
TAGGGCC500.007026983418.4999984
GTCCATT500.007026983418.49999811
TCCTCGG1156.373739E-817.69565220
Produced by FastQC (version 0.11.5)