##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632962.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	397358
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.710457572264808	33.0	33.0	33.0	27.0	33.0
2	31.208726136129133	33.0	33.0	33.0	27.0	33.0
3	31.642216842243016	33.0	33.0	33.0	27.0	33.0
4	31.771171085016533	33.0	33.0	33.0	27.0	33.0
5	31.888521182409818	33.0	33.0	33.0	33.0	33.0
6	34.7437550017868	37.0	37.0	37.0	27.0	37.0
7	34.91992359534727	37.0	37.0	37.0	27.0	37.0
8	35.07879041066243	37.0	37.0	37.0	33.0	37.0
9	35.161481585874704	37.0	37.0	37.0	33.0	37.0
10	35.21168316732015	37.0	37.0	37.0	33.0	37.0
11	35.238147967324174	37.0	37.0	37.0	33.0	37.0
12	35.209194227875116	37.0	37.0	37.0	33.0	37.0
13	35.2175921964576	37.0	37.0	37.0	33.0	37.0
14	35.18564115986088	37.0	37.0	37.0	33.0	37.0
15	35.21398587671571	37.0	37.0	37.0	33.0	37.0
16	35.203486528520884	37.0	37.0	37.0	33.0	37.0
17	35.16574474403435	37.0	37.0	37.0	33.0	37.0
18	35.18584752288868	37.0	37.0	37.0	33.0	37.0
19	35.197094307903704	37.0	37.0	37.0	33.0	37.0
20	35.19002008264587	37.0	37.0	37.0	33.0	37.0
21	35.180099557577805	37.0	37.0	37.0	33.0	37.0
22	35.17650330432507	37.0	37.0	37.0	33.0	37.0
23	35.16942152920037	37.0	37.0	37.0	33.0	37.0
24	35.155977732925976	37.0	37.0	37.0	33.0	37.0
25	35.17199855042556	37.0	37.0	37.0	33.0	37.0
26	35.11440061606913	37.0	37.0	37.0	33.0	37.0
27	35.061136305296486	37.0	37.0	37.0	33.0	37.0
28	35.0484625954429	37.0	37.0	37.0	27.0	37.0
29	35.07841795056347	37.0	37.0	37.0	33.0	37.0
30	35.02216640913232	37.0	37.0	37.0	27.0	37.0
31	35.01211501970515	37.0	37.0	37.0	27.0	37.0
32	35.05011853290987	37.0	37.0	37.0	27.0	37.0
33	35.012323899355245	37.0	37.0	37.0	27.0	37.0
34	34.939835110907545	37.0	37.0	37.0	27.0	37.0
35	34.940051540424506	37.0	37.0	37.0	27.0	37.0
36	34.92002929348346	37.0	37.0	37.0	27.0	37.0
37	34.950691819467586	37.0	37.0	37.0	27.0	37.0
38	34.810118331580085	37.0	37.0	37.0	27.0	37.0
39	34.879718037638604	37.0	37.0	37.0	27.0	37.0
40	34.923172554723955	37.0	37.0	37.0	27.0	37.0
41	34.92250313319475	37.0	37.0	37.0	27.0	37.0
42	34.83422505649817	37.0	37.0	37.0	27.0	37.0
43	34.60272600526477	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	7.0
16	21.0
17	27.0
18	19.0
19	20.0
20	48.0
21	88.0
22	221.0
23	607.0
24	1176.0
25	2182.0
26	3601.0
27	5344.0
28	7385.0
29	9666.0
30	12666.0
31	16040.0
32	20228.0
33	26575.0
34	37319.0
35	67434.0
36	186681.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.28986455538834	20.933515872336784	14.109694532386413	24.66692503988846
2	15.282440519632171	23.941382833616036	38.80002415957399	21.97615248717781
3	17.5937064309766	27.375817273088753	31.38454491919126	23.64593137674339
4	12.679246422621414	18.47427257032701	39.62950286643279	29.21697814061879
5	13.343383045012306	37.74279113544964	35.77806411346947	13.135761706068582
6	28.47784617397913	40.40839746525803	18.059281554668587	13.054474806094252
7	25.148103221779856	33.161531918320506	24.30981633690526	17.380548522994378
8	23.603903784496598	36.656868617216716	21.35429511926273	18.384932479023952
9	25.540444636826237	15.968471755948036	20.61742811268428	37.873655494541445
10	14.290136350595684	28.706607140160763	34.91008108557019	22.09317542367336
11	32.68815526552882	24.37600350313823	25.068829619637707	17.867011611695247
12	21.89008400485205	26.12228771032671	31.572284942042188	20.415343342779053
13	27.554749117923887	22.806637842952703	27.15108290257148	22.487530136551925
14	21.571227960680293	21.730781813880682	28.50376738356847	28.194222841870552
15	23.05351848962397	29.480216832176527	25.077889459882524	22.388375218316984
16	22.202900155527256	29.49531656591789	25.56510753527046	22.736675743284394
17	21.829936732115623	28.340438596932742	27.06526608247475	22.76435858847689
18	22.22529809391028	27.78124512404431	28.284569582089702	21.708887199955708
19	24.036511156186613	26.923580247534968	28.446136733122273	20.593771863156146
20	23.849022795564707	26.836756778522137	28.195229490786645	21.11899093512651
21	22.42536956598332	27.80817298254974	27.63880430241747	22.127653149049472
22	21.887315720332797	27.844412343529008	27.887194922462864	22.381077013675327
23	22.01767675496655	27.440746128176606	28.34018693470372	22.201390182153123
24	22.583413445809573	27.73544259836218	27.5804186652842	22.100725290544045
25	22.627454335888544	27.41457325635825	28.174593188006785	21.783379219746422
26	22.525782795363376	27.42891800341254	27.75406560330986	22.29123359791422
27	22.321936389854994	27.565318931542844	27.569345527207208	22.543399151394965
28	21.95476119771088	27.684103503641555	28.30872915607588	22.052406142571687
29	22.71025120923701	27.725879433659316	27.54015270864057	22.0237166484631
30	22.259775819286386	28.028880757402646	27.84642564136119	21.864917781949778
31	22.69062155537324	27.446282697215107	27.839882423406603	22.023213324005052
32	21.72222529809391	27.50995324115785	28.064616793923868	22.703204666824377
33	21.974642513803673	27.287987155159833	28.446388395351295	22.2909819356852
34	22.128156473507516	27.2887421418469	27.880400042279252	22.70270134236633
35	22.205416777817486	27.91588441657146	27.880651704508274	21.998047101102784
36	22.572340307732574	27.529079570563574	27.72889938040759	22.16968074129626
37	22.83834728380956	27.075332571635652	27.52027139254778	22.566048752007006
38	22.645070691920132	27.570352176123293	27.77268860825754	22.01188852369903
39	22.333261190161014	27.297550319862694	28.085001434474705	22.28418705550159
40	22.15156106080663	27.60684319933159	28.141122111546768	22.100473628315022
41	22.029253217501598	27.159387756129235	28.391274367195322	22.420084659173845
42	22.272862255195566	27.277417341540875	27.878386744447077	22.571333658816485
43	22.002577021225193	26.957302986224008	28.288344515525043	22.751775477025753
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	17.0
2	19.0
3	89.0
4	159.0
5	159.0
6	210.0
7	261.0
8	230.0
9	199.0
10	299.0
11	399.0
12	399.0
13	601.5
14	804.0
15	1538.5
16	2273.0
17	2628.0
18	2983.0
19	2983.0
20	3019.0
21	3055.0
22	3783.5
23	4512.0
24	5905.0
25	7298.0
26	7298.0
27	9158.0
28	11018.0
29	13659.5
30	16301.0
31	17933.5
32	19566.0
33	19566.0
34	21341.0
35	23116.0
36	24615.0
37	26114.0
38	27524.0
39	28934.0
40	28934.0
41	29990.5
42	31047.0
43	30154.5
44	29262.0
45	29186.0
46	29110.0
47	29110.0
48	28256.0
49	27402.0
50	27566.5
51	27731.0
52	27391.0
53	27051.0
54	27051.0
55	24278.0
56	21505.0
57	19143.5
58	16782.0
59	14784.0
60	12786.0
61	12786.0
62	11211.0
63	9636.0
64	7917.5
65	6199.0
66	5256.5
67	4314.0
68	4314.0
69	3515.0
70	2716.0
71	2365.5
72	2015.0
73	1639.0
74	1263.0
75	1263.0
76	951.0
77	639.0
78	492.5
79	346.0
80	271.5
81	197.0
82	197.0
83	160.0
84	123.0
85	113.0
86	103.0
87	84.0
88	65.0
89	65.0
90	45.0
91	25.0
92	19.5
93	14.0
94	7.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	397358.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.52801002398962
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.54825288484389	62.050915813473296
2	5.35836196204117	7.34395764493844
3	1.346319388218467	2.7678176559398002
4	0.643150582184791	1.762953181715765
5	0.3766207454932362	1.2904535111201467
6	0.25901061311703155	1.064968913520218
7	0.1974603422663216	0.9472095019916835
8	0.1401746380576583	0.768471120153946
9	0.122818122378449	0.7574833369330143
>10	0.871803814562878	12.072553280881882
>50	0.10202018012962323	4.795078688655489
>100	0.03289781170514306	3.614167074719419
>500	3.696383337656524E-4	0.1307057899183642
>1k	7.392766675313048E-4	0.6332644860385862
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1429	0.35962532527343105	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1071	0.2695302472833062	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	516	0.12985771017571057	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	423	0.10645312287659994	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.516622290226949E-4	0.0	0.0
2	0.0	0.0	2.516622290226949E-4	0.0	0.0
3	0.0	0.0	2.516622290226949E-4	0.0	0.0
4	0.0	0.0	2.516622290226949E-4	0.0	0.0
5	0.0	0.0	5.033244580453898E-4	0.0	0.0
6	0.0	0.0	0.0015099733741361695	0.0	0.0
7	0.0	0.0	0.004278257893385813	0.0	0.0
8	0.0	0.0	0.005536569038499288	0.0	0.0
9	0.0	0.0	0.006039893496544678	5.033244580453898E-4	0.0
10	2.516622290226949E-4	0.0	0.007298204641658152	5.033244580453898E-4	0.0
11	2.516622290226949E-4	0.0	0.0098148269318851	5.033244580453898E-4	0.0
12	2.516622290226949E-4	0.0	0.010569813618953186	5.033244580453898E-4	0.0
13	2.516622290226949E-4	0.0	0.011576462535043965	5.033244580453898E-4	0.0
14	2.516622290226949E-4	0.0	0.012583111451134745	5.033244580453898E-4	0.0
15	2.516622290226949E-4	0.0	0.014596409283316304	5.033244580453898E-4	0.0
16	2.516622290226949E-4	0.0	0.017616356031588645	5.033244580453898E-4	0.0
17	2.516622290226949E-4	0.0	0.018623004947679423	7.549866870680847E-4	0.0
18	2.516622290226949E-4	0.0	0.018874667176702116	7.549866870680847E-4	0.0
19	2.516622290226949E-4	0.0	0.019126329405724812	7.549866870680847E-4	0.0
20	2.516622290226949E-4	0.0	0.01937799163474751	7.549866870680847E-4	0.0
21	2.516622290226949E-4	0.0	0.021139627237906373	7.549866870680847E-4	0.0
22	2.516622290226949E-4	0.0	0.021642951695951762	7.549866870680847E-4	0.0
23	2.516622290226949E-4	0.0	0.021894613924974455	0.0010066489160907796	0.0
24	2.516622290226949E-4	0.0	0.021894613924974455	0.0012583111451134744	0.0
25	2.516622290226949E-4	0.0	0.021894613924974455	0.0012583111451134744	0.0
26	2.516622290226949E-4	0.0	0.021894613924974455	0.0012583111451134744	0.0
27	2.516622290226949E-4	0.0	0.021894613924974455	0.002013297832181559	0.0
28	2.516622290226949E-4	0.0	0.021894613924974455	0.007801529099703542	0.0
29	2.516622290226949E-4	0.0	0.022397938383019848	0.020384640550838287	0.0
30	2.516622290226949E-4	0.0	0.022397938383019848	0.04026595664363118	0.0
31	2.516622290226949E-4	0.0	0.022397938383019848	0.10016156715103257	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	280	0.0	29.732141	1
CGAACTA	60	1.3337303E-6	24.666668	29
CGGCCTT	60	1.3337303E-6	24.666668	24
GCTTCGC	45	1.3210849E-4	24.666666	22
GTATCAA	905	0.0	24.325966	1
GCGAACT	65	2.675517E-6	22.76923	28
TTCGCCG	50	2.6977935E-4	22.199999	24
CGAGGGT	45	0.0038214703	20.555555	16
AGACCGC	45	0.0038214703	20.555555	14
CGCCGGT	45	0.0038214703	20.555555	36
CTATCAC	65	6.8876136E-5	19.923077	3
TCTCGCC	85	1.2411165E-6	19.588234	12
TTCCTCG	85	1.2411165E-6	19.588234	19
AGCTTCG	60	9.222322E-4	18.5	21
GCCGGCA	60	9.222322E-4	18.5	15
CCTCGGC	110	3.8327926E-8	18.5	21
GACTGTG	110	3.8327926E-8	18.5	7
TAGGGCC	50	0.0070269834	18.499998	4
GTCCATT	50	0.0070269834	18.499998	11
TCCTCGG	115	6.373739E-8	17.695652	20
>>END_MODULE