FastQCFastQC Report
Fri 10 Feb 2017
ERR1632961.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632961.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428619
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13070.3049328191237439No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8850.2064770810440041No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5060.11805356272120461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6300.025.547621
CATCGTG400.001929222623.12536
TAAGGGT652.6763337E-622.769234
GGTATCA1850.022.01
CGACGAT450.003821952320.55555737
CGAACTA555.1358645E-420.18181829
TAACGCT555.1358645E-420.18181823
CGGTCAC555.1358645E-420.18181830
TTCGCCG656.889232E-519.92307724
ACTGTCC759.243555E-619.7333348
CGTCAGA759.243555E-619.73333412
CTATCCC701.2169349E-418.54
GCGAACT609.223936E-418.528
GCCGGCA701.2169349E-418.515
TTACTTA609.223936E-418.529
TTCGGTC609.223936E-418.528
GTATAAA801.6128191E-518.51
CAAAGCG701.2169349E-418.524
GTTTCGG500.007027863518.49999830
TTATGCG500.007027863518.4999984
Produced by FastQC (version 0.11.5)