FastQCFastQC Report
Fri 10 Feb 2017
ERR1632960.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632960.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412997
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15490.37506325711809047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10760.2605345801543353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5810.14067898798296358No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4450.10774896669951597No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4390.10629617164289329No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4150.10048499141640255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3100.025.6612911
CGCTCTC901.4188117E-1024.66666829
GTATCAA9450.023.2962951
GATTAGG400.001929103623.1251
TGCGGTA502.698041E-422.236
GTACTAT502.698041E-422.21
CCGCTCT1059.767973E-1021.14285728
GTCGCCC806.9379166E-720.812537
ATCTTAC806.9379166E-720.81251
GAATCAA450.003821719720.5555571
TTCGCCG1101.7444108E-920.18181824
AGCTTCG1202.3646862E-1020.04166621
GCCGGCA1202.3646862E-1020.04166615
TTTACAC656.88845E-519.9230773
GTGTATA656.88845E-519.9230771
GTTTACC759.242212E-619.73333415
CTTACCC851.2413584E-619.5882363
TCTCGTC1052.2489985E-819.3809512
GCCGCTC1153.0340743E-919.30434827
GCTTCGC1254.0927262E-1019.24000222
Produced by FastQC (version 0.11.5)