FastQCFastQC Report
Fri 10 Feb 2017
ERR1632959.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632959.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383184
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18360.4791431792559188No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13340.3481356215290827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7420.19364065305440728No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6610.17250198338135203No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG5430.14170737817862958No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5140.13413921249321475No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4820.12578813311620526No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4740.12370036327195291No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT4720.12317842081088981No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG4590.1197857948139797No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT4520.11795899620025889No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC4290.11195665789803333No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC4270.11143471543697023No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG4120.10752014697899703No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4100.10699820451793393No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG4030.10517140590421313No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT3920.1023007223683661No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG3910.10203975113783456No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3890.10151780867677149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4150.028.530121
GTCGCCC951.0913936E-1125.3157937
GTATCAA11050.022.2669681
GCACCGC855.1581083E-821.76470610
AGGTCGC1059.767973E-1021.14285735
ACATACC450.00382122520.5555553
CGGTTCA656.886794E-519.9230778
CACCGCC951.6688318E-719.47368411
CGGAGTT951.6688318E-719.47368424
GGTCGCC1153.0322553E-919.30434836
TATTAGA1355.4569682E-1119.1851862
TTAAGGT609.2215056E-418.54
CGGGATA801.6120908E-518.517
TGGGGCG801.6120908E-518.537
CATACCT701.216507E-418.54
GGTTCAC1409.276846E-1118.56
CTAGTAG500.007026535518.4999983
CATGCTA1950.018.0256424
GTATTAG1351.1441443E-917.8148161
GGCACCG1054.776666E-717.6190479
Produced by FastQC (version 0.11.5)