##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632959.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	383184
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.758050962461898	33.0	33.0	33.0	27.0	33.0
2	31.28509019165727	33.0	33.0	33.0	27.0	33.0
3	31.701696834940915	33.0	33.0	33.0	27.0	33.0
4	31.82891770011274	33.0	33.0	33.0	27.0	33.0
5	31.931158399098084	33.0	33.0	33.0	33.0	33.0
6	34.79224341308614	37.0	37.0	37.0	27.0	37.0
7	34.91148377802831	37.0	37.0	37.0	27.0	37.0
8	35.130981460603785	37.0	37.0	37.0	33.0	37.0
9	35.17214445279552	37.0	37.0	37.0	33.0	37.0
10	35.22092258549417	37.0	37.0	37.0	33.0	37.0
11	35.24991648920623	37.0	37.0	37.0	33.0	37.0
12	35.15888711428452	37.0	37.0	37.0	33.0	37.0
13	35.228788258382394	37.0	37.0	37.0	33.0	37.0
14	35.17575890433839	37.0	37.0	37.0	33.0	37.0
15	35.22978777819533	37.0	37.0	37.0	33.0	37.0
16	35.22970687711387	37.0	37.0	37.0	33.0	37.0
17	35.20844816067477	37.0	37.0	37.0	33.0	37.0
18	35.185112113240635	37.0	37.0	37.0	33.0	37.0
19	35.19981001294417	37.0	37.0	37.0	33.0	37.0
20	35.13433494091611	37.0	37.0	37.0	33.0	37.0
21	35.203781995072866	37.0	37.0	37.0	33.0	37.0
22	35.20656916781494	37.0	37.0	37.0	33.0	37.0
23	35.19023497849597	37.0	37.0	37.0	33.0	37.0
24	35.16138460896071	37.0	37.0	37.0	33.0	37.0
25	35.1862734352165	37.0	37.0	37.0	33.0	37.0
26	35.13034208108898	37.0	37.0	37.0	33.0	37.0
27	35.07172533299929	37.0	37.0	37.0	33.0	37.0
28	35.07823917491336	37.0	37.0	37.0	33.0	37.0
29	35.0943645872479	37.0	37.0	37.0	33.0	37.0
30	35.062074616894236	37.0	37.0	37.0	27.0	37.0
31	35.03999384107896	37.0	37.0	37.0	27.0	37.0
32	35.068369242974654	37.0	37.0	37.0	27.0	37.0
33	35.04777600317341	37.0	37.0	37.0	27.0	37.0
34	34.99752599273456	37.0	37.0	37.0	27.0	37.0
35	34.98387980709007	37.0	37.0	37.0	27.0	37.0
36	34.96248538561109	37.0	37.0	37.0	27.0	37.0
37	34.98639817946469	37.0	37.0	37.0	27.0	37.0
38	34.84101110693557	37.0	37.0	37.0	27.0	37.0
39	34.882876111737446	37.0	37.0	37.0	27.0	37.0
40	34.924279197461274	37.0	37.0	37.0	27.0	37.0
41	34.95395423608501	37.0	37.0	37.0	27.0	37.0
42	34.80831924088688	37.0	37.0	37.0	27.0	37.0
43	34.63033686166437	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	11.0
16	12.0
17	16.0
18	20.0
19	17.0
20	34.0
21	62.0
22	203.0
23	557.0
24	1037.0
25	2092.0
26	3322.0
27	4914.0
28	6957.0
29	9402.0
30	12001.0
31	15665.0
32	19542.0
33	25157.0
34	35869.0
35	65224.0
36	181068.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.84095369326485	21.21565618606205	13.74065722994697	24.202732890726125
2	15.800503152532464	23.335003549208736	38.801202555430294	22.063290742828514
3	17.210008768633344	28.07215332581736	30.739801244310826	23.978036661238463
4	11.989018330619231	18.51512589252161	40.82190279343605	28.673952983423106
5	13.042559188275085	37.70668921458098	36.00907135997328	13.241680237170655
6	27.770209612092362	41.733736272913276	17.62443108271744	12.871623032276922
7	25.51489623783874	33.2263351288154	24.087122635600654	17.17164599774521
8	23.606935571422607	36.604346736815735	20.943724163848177	18.844993527913484
9	25.859900204601445	15.438275084554679	20.81584826088772	37.88597644995615
10	14.833604743413087	28.354263226021963	34.385830306067064	22.42630172449789
11	33.374044845296254	24.369232535805253	24.52111779197461	17.73560482692388
12	22.825065764750093	26.63994321266024	30.54276796525951	19.99222305733016
13	27.57422021796317	23.422167940206272	26.428034573468622	22.575577268361936
14	21.712806380224645	21.576840369117708	28.69509165309616	28.015261597561487
15	23.463401394630257	29.154661990062213	25.63416009019166	21.74777652511587
16	22.00587707211157	29.360046348490542	25.915226105474133	22.718850473923755
17	21.624859075535515	28.120954945926762	26.766253288237507	23.48793269030022
18	22.543216835776025	27.59953442732473	28.536682116163515	21.320566620735732
19	24.40994404776817	26.756336381477308	28.04710008768633	20.786619483068186
20	23.7682157918911	26.611758319762828	28.47457096329701	21.145454925049062
21	22.30260136122594	27.938797027015745	28.33181970019625	21.42678191156207
22	22.05963714560107	27.674433170487283	28.08885548457138	22.177074199340264
23	22.16454758027475	27.419203307027434	28.920048853814357	21.496200258883462
24	22.303123303687002	28.035617353542946	27.00973944632344	22.651519896446615
25	22.517641655183933	27.675477055409413	28.4703954236085	21.336485865798156
26	22.346183556724707	27.970374545910058	28.18593678232912	21.49750511503612
27	22.494415215666624	27.654599356966887	27.706793603073198	22.14419182429329
28	21.862864837780283	27.365965175998998	28.810179965760575	21.960990020460144
29	23.40285606914694	27.42311787548541	27.598229571172073	21.575796484195582
30	22.827153534594345	27.026441605077455	28.504582654808136	21.641822205520064
31	22.481627625370578	27.26862290701073	28.164276170194995	22.08547329742369
32	21.37667543530001	27.088552757943962	28.71048895569752	22.8242828510585
33	21.523080295628212	27.253747546870432	29.02261054741325	22.200561610088105
34	22.27206772725375	26.96928890559105	28.788258382395927	21.97038498475928
35	22.172115745960166	28.023090734477428	28.011868971564574	21.79292454799783
36	22.882479435467033	27.647031191281474	27.684350077247483	21.78613929600401
37	22.36001503194288	27.001388366946426	27.97246231575431	22.66613428535638
38	22.465708380308154	27.398325608584912	27.86389828385319	22.27206772725375
39	21.925497933107856	27.273842331621363	28.60818823332916	22.192471501941625
40	22.01031358303061	28.00090817988225	28.45682491962086	21.531953317466282
41	22.224570963297005	26.5527788216627	28.4703954236085	22.752254791431792
42	21.554396843291997	27.799699361142427	28.423159630882296	22.222744164683288
43	22.628032485698775	26.45726335128815	28.27440602947931	22.640298133533758
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	24.0
2	32.0
3	97.0
4	162.0
5	162.0
6	207.0
7	252.0
8	251.0
9	250.0
10	334.5
11	419.0
12	419.0
13	647.0
14	875.0
15	1648.0
16	2421.0
17	2824.5
18	3228.0
19	3228.0
20	3086.0
21	2944.0
22	3330.5
23	3717.0
24	4932.0
25	6147.0
26	6147.0
27	7667.5
28	9188.0
29	10925.0
30	12662.0
31	15148.5
32	17635.0
33	17635.0
34	20232.0
35	22829.0
36	24500.5
37	26172.0
38	27812.5
39	29453.0
40	29453.0
41	30517.0
42	31581.0
43	32155.0
44	32729.0
45	31282.0
46	29835.0
47	29835.0
48	28873.5
49	27912.0
50	27246.0
51	26580.0
52	25065.5
53	23551.0
54	23551.0
55	22749.5
56	21948.0
57	18575.0
58	15202.0
59	13606.0
60	12010.0
61	12010.0
62	10439.0
63	8868.0
64	7079.5
65	5291.0
66	4438.0
67	3585.0
68	3585.0
69	2983.5
70	2382.0
71	1911.5
72	1441.0
73	1138.5
74	836.0
75	836.0
76	619.0
77	402.0
78	306.5
79	211.0
80	189.0
81	167.0
82	167.0
83	133.5
84	100.0
85	87.0
86	74.0
87	60.5
88	47.0
89	47.0
90	33.0
91	19.0
92	14.5
93	10.0
94	5.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	383184.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.28346275196142
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.3208579848722	55.35174936041113
2	5.143921003025147	6.304745823758472
3	1.3279074409966753	2.441362985951165
4	0.6883057756252606	1.687270454499623
5	0.43351076408794437	1.328352038177894
6	0.306417925602145	1.1267010918103404
7	0.21489496575827477	0.921865534074188
8	0.16905115400996565	0.8288032079956659
9	0.13789920784071008	0.7605846870508028
>10	1.0406713913231982	12.584048952244292
>50	0.13122394134395896	5.641652137725489
>100	0.08276542705675544	9.543267064663103
>500	0.0017153456384819779	0.6465022713372961
>1k	8.576728192409889E-4	0.8330943903004994
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1836	0.4791431792559188	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1334	0.3481356215290827	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	742	0.19364065305440728	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	661	0.17250198338135203	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	543	0.14170737817862958	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	514	0.13413921249321475	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	482	0.12578813311620526	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	474	0.12370036327195291	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	472	0.12317842081088981	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	459	0.1197857948139797	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	452	0.11795899620025889	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	429	0.11195665789803333	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	427	0.11143471543697023	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	412	0.10752014697899703	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	410	0.10699820451793393	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	403	0.10517140590421313	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	392	0.1023007223683661	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	391	0.10203975113783456	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	389	0.10151780867677149	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.609712305315462E-4	0.0	0.0
2	2.609712305315462E-4	0.0	2.609712305315462E-4	0.0	0.0
3	2.609712305315462E-4	0.0	2.609712305315462E-4	0.0	0.0
4	2.609712305315462E-4	0.0	5.219424610630924E-4	0.0	0.0
5	2.609712305315462E-4	0.0	7.829136915946386E-4	0.0	0.0
6	2.609712305315462E-4	0.0	0.001304856152657731	0.0	0.0
7	2.609712305315462E-4	0.0	0.0039145684579731934	2.609712305315462E-4	0.0
8	5.219424610630924E-4	0.0	0.004958453380099378	2.609712305315462E-4	0.0
9	5.219424610630924E-4	0.0	0.006263309532757109	2.609712305315462E-4	0.0
10	7.829136915946386E-4	0.0	0.0067852519938202014	2.609712305315462E-4	0.0
11	7.829136915946386E-4	0.0	0.009133993068604117	2.609712305315462E-4	0.0
12	7.829136915946386E-4	0.0	0.009394964299135663	2.609712305315462E-4	0.0
13	7.829136915946386E-4	0.0	0.009394964299135663	2.609712305315462E-4	0.0
14	7.829136915946386E-4	0.0	0.01096079168232494	2.609712305315462E-4	0.0
15	7.829136915946386E-4	0.0	0.012526619065514218	2.609712305315462E-4	0.0
16	0.0010438849221261848	0.0	0.013309532757108857	2.609712305315462E-4	0.0
17	0.0010438849221261848	0.0	0.013309532757108857	2.609712305315462E-4	0.0
18	0.0010438849221261848	0.0	0.013309532757108857	2.609712305315462E-4	0.0
19	0.0010438849221261848	0.0	0.013570503987640403	5.219424610630924E-4	0.0
20	0.0010438849221261848	0.0	0.013831475218171949	5.219424610630924E-4	0.0
21	0.0010438849221261848	0.0	0.014092446448703494	5.219424610630924E-4	0.0
22	0.0010438849221261848	0.0	0.014353417679235042	7.829136915946386E-4	0.0
23	0.0010438849221261848	0.0	0.014353417679235042	0.0010438849221261848	0.0
24	0.0010438849221261848	0.0	0.014353417679235042	0.001304856152657731	0.0
25	0.0010438849221261848	0.0	0.014614388909766587	0.0020877698442523696	0.0
26	0.0010438849221261848	0.0	0.014614388909766587	0.0023487410747839157	0.0
27	0.0010438849221261848	0.0	0.014614388909766587	0.0028706835358470084	0.0
28	0.0010438849221261848	0.0	0.014614388909766587	0.010699820451793395	0.0
29	0.0010438849221261848	0.0	0.014614388909766587	0.023748381978370703	0.0
30	0.0010438849221261848	0.0	0.014614388909766587	0.04384316672929976	0.0
31	0.0010438849221261848	0.0	0.014614388909766587	0.10882500313165476	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	415	0.0	28.53012	1
GTCGCCC	95	1.0913936E-11	25.31579	37
GTATCAA	1105	0.0	22.266968	1
GCACCGC	85	5.1581083E-8	21.764706	10
AGGTCGC	105	9.767973E-10	21.142857	35
ACATACC	45	0.003821225	20.555555	3
CGGTTCA	65	6.886794E-5	19.923077	8
CACCGCC	95	1.6688318E-7	19.473684	11
CGGAGTT	95	1.6688318E-7	19.473684	24
GGTCGCC	115	3.0322553E-9	19.304348	36
TATTAGA	135	5.4569682E-11	19.185186	2
TTAAGGT	60	9.2215056E-4	18.5	4
CGGGATA	80	1.6120908E-5	18.5	17
TGGGGCG	80	1.6120908E-5	18.5	37
CATACCT	70	1.216507E-4	18.5	4
GGTTCAC	140	9.276846E-11	18.5	6
CTAGTAG	50	0.0070265355	18.499998	3
CATGCTA	195	0.0	18.025642	4
GTATTAG	135	1.1441443E-9	17.814816	1
GGCACCG	105	4.776666E-7	17.619047	9
>>END_MODULE