FastQCFastQC Report
Fri 10 Feb 2017
ERR1632958.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632958.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446233
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25550.5725708318300081No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18320.41054785280335604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9170.20549802457460564No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4710.10555023944889777No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG4560.10218876685498383No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAT250.00549278529.5999983
TCTATCG358.860857E-426.42857229
GGTATCA4050.025.5802461
GCCGGCA1500.024.66666615
TTCGCCG1350.024.66666624
CCGGCAG1450.024.24137916
CGCTCTC1450.024.24137929
AGCTTCG1550.022.67741821
GTATCAA10750.022.5441881
GCCGCTC1400.022.46428727
GCGAACC855.1628376E-821.76470630
GCTTCGC1650.021.3030322
GAGTCGC1251.8189894E-1120.7222
GGCGAGT1251.8189894E-1120.7219
CGCCGCT1700.020.67647226
AACCGGG909.445648E-820.55555533
TATTGCG450.00382219620.5555559
TATCGCC450.00382219620.55555531
CGTCCGC1001.2843884E-820.34999829
CCGCTCT1550.020.29032128
Produced by FastQC (version 0.11.5)